Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance.

In order to study the intracellular polyamine distribution in Escherichia coli, 13C-NMR spectra of [1,4-13C]putrescine were obtained after addition of the latter to intact bacteria. The 13C-enriched methylene signal underwent line broadening. When the cells were centrifuged after 90 min the cell-bound putrescine peak had a linewidth of 23 Hz, while the supernatant liquid showed an unbound putrescine signal with a linewidth smaller than 1 Hz.

Effects of short-term thyroxine treatment on pancreatic cytology and responses of blood sugar, serum insulin and serum free fatty acids to epinephrine infusion.

The influence of short-term treatment with l-thyroxine on pancreatic histology and on the responses of glycemia, insulinemia and serum free fatty acids to a continuous l-epinephrine infusion in the absence or presence of alpha- and beta-adrenergic blockade was studied in male dogs. l-Epinephrine dosage: 0.06 microgram/kg body weight/min for 55 min.

Blood sugar, serum insulin and serum free fatty acid responses to graded glucose pulses in hypothyroid dogs.

The actions of hypothyroidism on BS, serum IRI and circulating FFA profiles observed in response to single glucose pulses at three levels of stimulation (1.00, 0.66 and 0.

Blood sugar, serum insulin and serum free fatty acid responses to slow graded glucose in thyroxine-treated dogs.

The effects of short-term (10 days) thyroxine administration (100 micrograms/kg body weight/die) on the BS, serum IRI and circulating FFA responses to slow, graded glucose stimulation were studied in dogs. The experiments reported demonstrated that the mean basal BS value in thyroxine-treated dogs is higher than that found in untreated controls, and that non-parallel mean BS responses to glucose infusion were observed during the test: the higher curve was found in the thyroxine-treated group. Mean basal serum IRI was similar in both groups, and parallel insulinemic responses to hyperglycemia were observed.