Human T cell generation is restored in CD3δ severe combined immunodeficiency through adenine base editing.

CD3δ SCID is a devastating inborn error of immunity caused by mutations in CD3D, encoding the invariant CD3δ chain of the CD3/TCR complex necessary for normal thymopoiesis. We demonstrate an adenine base editing (ABE) strategy to restore CD3δ in autologous hematopoietic stem and progenitor cells (HSPCs). Delivery of mRNA encoding a laboratory-evolved ABE and guide RNA into a CD3δ SCID patient's HSPCs resulted in a 71.

Serum IgG Responses to gp15 and gp40 Protein-Derived Synthetic Peptides From .

spp. are responsible for moderate to severe diarrhea, mainly in children and immunocompromised patients. Using ELISA, the recognition of synthetic peptides generated from the sequences of the gp40 and gp15 proteins by serum IgM and IgG antibodies from patients infected (cases) with , , and , and uninfected individuals (controls) was evaluated.

Dynamics and genetic regulation of leaf nutrient concentration in barley based on hyperspectral imaging and machine learning.

Biofortification, the enrichment of nutrients in crop plants, is of increasing importance to improve human health. The wild barley nested association mapping (NAM) population HEB-25 was developed to improve agronomic traits including nutrient concentration. Here, we evaluated the potential of high-throughput hyperspectral imaging in HEB-25 to predict leaf concentration of 15 mineral nutrients, sampled from two field experiments and four developmental stages.

Cellulose defects in the Arabidopsis secondary cell wall promote early chloroplast development.

Lincomycin (LIN)-mediated inhibition of protein synthesis in chloroplasts prevents the greening of seedlings, represses the activity of photosynthesis-related genes in the nucleus, including LHCB1.2, and induces the phenylpropanoid pathway, resulting in the production of anthocyanins. In genomes uncoupled (gun) mutants, LHCB1.

Pourfour du Petit Syndrome Associated With Right Eye Pressure.

Pourfour du Petit (PDP) syndrome is a rare disorder characterized by ipsilateral mydriasis, eyelid retraction, and hemifacial hyperhidrosis caused by hyperactivity of the ipsilateral oculosympathetic pathway. A case is presented of PDP syndrome associated with likely ipsilateral occipital neuralgia. We review the causes and co-morbidities and the clinical features of PDP.

[Computerised clinical records: 8 years experience in a surgical/medical department].

Background: The use of Electronic Health Records (EHR) has been irregular due to having to overcome barriers to their introduction. We describe our 8 years experience in using EHR to monitor quality control in the Urology Department of a tertiary Hospital.

Methods: Retrospective analysis of the development and implementation of the EHR from 2001 to 2008 and the structural changes in the health care process and their results.

Two different chitinase genotypes in a patient with an amebic liver abscess: a case report.

The present work deals with the identification of a patient with two liver abscesses containing two different strains of Entamoeba histolytica, as defined by chitinase gene plymorphisms.

Growth of faecal coliforms and Salmonella spp. in physicochemical sludge treated with acetic acid.

The use of advanced primary treatment (APT) to remove helminth ova from wastewater has raised the issue of treating the generated sludge to allow its reuse or disposal. Several studies have been performed in Mexico in order to treat the sludge with the main goal of destroying helminth ova and bacteria, one of them analysing the acid treatment. Previous research has demonstrated the feasibility of applying such a process using acetic acid to disinfect the sludge, but the potential for bacterial growth was still to be proved.