PacBio Genome Sequences of Serotype O157:H7, Diffusely Adherent , and Strains, All Carrying Plasmids with an Resistance Gene.

We report here Illumina-corrected PacBio whole-genome sequences of an serotype O157:H7 strain (2017C-4109), an serotype O[undetermined]:H2 strain (2017C-4173W12), and a subsp. serovar Enteritidis strain (2017K-0021), all of which carried the resistance gene on an IncI2 or IncX4 plasmid. We also determined that pMCR-1-CTSe is identical to a previously published plasmid, pMCR-1-CT.

High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing.

Shiga toxin-producing (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.

High-Quality Whole-Genome Sequences for 59 Historical Shigella Strains Generated with PacBio Sequencing.

spp. are enteric pathogens that cause shigellosis. We report here the high-quality whole-genome sequences of 59 historical strains that represent the four species and a variety of serotypes.

High-Quality Whole-Genome Sequences for 21 Enterotoxigenic Strains Generated with PacBio Sequencing.

Enterotoxigenic (ETEC) is an important diarrheagenic pathogen. We report here the high-quality whole-genome sequences of 21 ETEC strains isolated from patients in the United States, international diarrheal surveillance studies, and cruise ship outbreaks.

High-Quality Complete and Draft Genome Sequences for Three spp. and Three spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping.

spp., including and , , and are causative agents of foodborne disease. We report here reference-level whole-genome sequences of (2014C-4356), (2011C-4315 and 2012C-4431), (BU53M1), and (94-3007 and 71-2783).

High-Quality Draft Genome Sequences for Four Drug-Resistant or Outbreak-Associated Strains Generated with PacBio Sequencing and Whole-Genome Maps.

Drug-resistant poses a clinical and public health challenge. We report here the high-quality draft whole-genome sequences of four outbreak-associated isolates; three were resistant to two or more antibiotics, and one was resistant to streptomycin only.

Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii.

Escherichia coli, Escherichia albertii, and Escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. Current identification strategies for these three species vary in complexity and typically rely on the use of multiple phenotypic and genetic tests. To facilitate their rapid identification, we developed a multiplex PCR assay targeting conserved, species-specific genes.

High-Quality Genome Sequence of an O157 Strain Carrying an Resistance Gene Isolated from a Patient in the United States.

carrying plasmid-mediated colistin resistance have been found around the world. We report here the high-quality whole-genome sequence of an O157:H48 isolate (2016C-3936C1) from Connecticut that carried the resistance gene on an IncX4-type plasmid.

High-Quality Draft Genome Sequences for Five Non-O157 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing and Optical Maps.

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen. We report here the high-quality draft whole-genome sequences of five STEC strains isolated from clinical cases in the United States. This report is for STEC of serotypes O55:H7, O79:H7, O91:H14, O153:H2, and O156:H25.

Complete Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains from Serotypes O119:H4 and O165:H25.

Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Here, we report complete whole-genome sequences for two STEC strains of serotypes O119:H4 and O165:H25 isolated from clinical cases in the United States.