Publications by authors named "Garcia-Segura L"

The effect that being subjected to different environmental situations has in the adult mouse is analysed. Adult mice are placed for that, in groups of twelve mice in cages that contains ramps, catwalks, passages, sleeping boxes and play objects, that were changed in their relative position several times in the week. Other mice were placed in groups of four mice in smaller boxes and without these objects.

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In this work we research into the GDH location of the spinal cord, in the medullar oblongata and in the central cerebellar nuclei of the rat, cat, dog, chicken, lizard (Lacerta lepida), and Toad (Bufo calamita). The GDH is located at the cytoplasm and at the neuronal prolongations, at oligodendrocytes cells, at Lecerta ependimary glial cells, and at the capillary walls.

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A histochemical study on Lacerta lepida cerebral cortex glutamic dehydrogenase activity (GLD) was reported in this paper. The GLD activity is revealed in neurons and fibers of cerebral cortex. The regions M1 and M2 were differentiated with regard to enzymatical activity.

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In this work is studied the tannin-iron method specificity when the ferrous chloride is replaced by other iron compounds. Nervous tissue and rat duodenum cuts are used. Cuts were treated with tannic acid and after with FeCl3; FeCl2; FeOHCl2; FeAc2; Fe2O3; FeSO4; NH4Fe(SO4)2 or colloidal iron solution.

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Union of several iron compounds and other metallic cations to the duodenum mucines and to the neuronal surface mucopolysaccharides is studied. The results differentiate clearly the behaviour of the duodenum mucosubstances and neuronal surface mucosubstances. The duodenum mucines only attract to certain cations, in such a way that their union seems to be specific.

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A histochemical study of mucopolysaccharides in the dog spinal cord and dorsal root ganglia is reported in this paper. The histochemical techniques used were the following: PAS, colloidal iron, toluidine blue (pH 5.4 and 3.

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The most recent knowledge about the phenomenon of the nervous system plasticity are revised, as much in morphological as in physiological and molecular levels. The neuron morphological and physiological changes opposite to the experience are studied. The nervous system molecular adaptation to the information it receives as the base of all type of plasticity is also considered.

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A study of the hypothalamic nuclei of the rat, using a variety of histochemical methods, has shown the distribution of mucopolysaccharides and neurosecretory material. In all the hypothalamic nuclei there are two types of neurons and in some a third type characterized by the presence of hyaluronidase-resistant acid mucopolysaccharides in the periphery of the perikaryon. These type III neurons also contain Gomori-positive material and neutral mucopolysaccharides.

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The aspartate aminotransferase activity (AAT) is reserched into the spinal cord, the medulla oblongata and the cerebellar nuclei of the rat, chicken, Lacerta lepida and Bufo calamitas. It's proved that the AAT activity shows in many locations, that are mainly: 1. In the nerve fibers 2.

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The histochemical study of the consumption of glutamic acid by way of the aspartate aminotransferase and the glutamic dehydrogenase in the cerebellar cortex of several species of animals have demonstrated that in that nerve centre exists some structures in which the mentioned consumption is specially or exclusively realized by means of one way and not for other different one. Is observed, as well, that in the rats, chicken and lizard, the baskets that surround the Purkinje cells are constituted by basket cells axons and by recurrent collaterals of Purkinje axons and that those structures have an intense aspartate aminotransferase activity, but not glutamic dehydrogenase. The aspartate aminotransferase activity was not observed on the other side, in the perikarya of the Purkinje cells of the related animals.

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In many cases, in a fixed neuronal circuit, the complete morphological and biochemical development of a neuron depends on the impulses it receives from the neuron before. The possible involvement of this phenomenon to learning is studied in this work.

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Histological sections of the Subthalamic region of rats were treated with solutions of: Toluidin Blue, Thionin, Alcian Blue and Colloidal Iron. The microscopic observation of the histological sections treated with those histochemic reactions showed the existence in the Subthalamic region of three neuronal types, differentiated between them because of the morphologic and histochemic points of view. In the LUY's Subthalamic nucleus was observed only one neuronal type, while in the nucleus of the FOREL's field and in the Zona incerta were found two very special types of neurons (alpha and beta) differentiated between them because of their reaction before the Collidal Iron.

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A histochemical study in the dog Superior Cervical ganglion and in the Cervicothoracic or Stellate ganglion is reported. The mucopolysaccharide composition of these ganglia are studied by means of five histochemical techniques. The different histochemical composition between the neurons of these ganglia is investigated.

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