This in-vitro study aimed to explore the potential of Diode Laser by quantifying the release of transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF)-BB, and vascular endothelial growth factor (VEGF) from root dentin after ethylenediaminetetraacetic acid (EDTA) and diode laser. In 30 hemi-roots distributed into five groups: G1: Tris-buffered solution (TBS); G2: 17%EDTA; G3: 17% EDTA +650 nm Diode Laser; G4:17% EDTA +810 nm Diode Laser; and G5: 810 nm Diode Laser. The concentration of the three factors was quantified using a cytokine bead array.
View Article and Find Full Text PDFBackground: Disinfection and dentin conditioning promote a favorable scenario for regenerative endodontic treatment. Clinical reports have confirmed periapical normality with high variability in disinfection protocols; nevertheless, the nature of neoformed tissue varied between them. Thus, this study aimed to present the impact of disinfection protocols on the clinical, histological, and molecular outcomes of regenerative endodontics procedures in permanent teeth with incomplete root formation.
View Article and Find Full Text PDFIntroduction: The presented study aimed to characterise periapical disease in teeth with primary non-surgical root canal treatment in persistent or emergent categories and their risk association.
Methods: A retrospective observational study that evaluated permanent teeth with primary non-surgical root canal treatment, was conducted clinically and radiographically for over one year. The following variables were analysed: gender, age, type and location of tooth, previous diagnosis, treatment conditions, and type of coronal restoration.
Objective: To identify the effect of two chitosan solutions on the release of root dentin matrix proteins and to describe the chemical changes observed following conditioning with chelating agents.
Materials And Methods: The release of dentin sialoprotein (DSP), transforming growth factor-beta 1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) with different chelating agents, including ethylenediaminetetraacetic acid (EDTA), chitosan solution (CS), and nanoparticulate chitosan (CSnp), was investigated. DSP was quantified using an enzyme-linked immunosorbent assay (ELISA).
Background: This nested case-control study can be viewed as an efficient way to sample subjects from a large cohort study case-control study aimed to analyze the effect of different clinical factors on the appearance of vertical root fractures in endodontically-treated teeth (ETT) over time.
Material And Methods: By matching 90 cases and 270 controls nested in a cohort of 450 patients. Incident "cases" included those ETT in which a confirmed VRF.
Aims: To compare the effect of CS and CSnp on the wettability in root dentine with other irrigation protocols with an experimental model prior regenerative endodontics. . An experimental study that included eighty hemisected human root distributed into 8 groups: G1- distilled water; G2- 1% NaOCl/17% EDTA; G3- hypochlorous acid 0.
View Article and Find Full Text PDFOdontology
October 2020
Aim: The prevalence of vertical root fractures (VRF) ranges from 2% to 20%, and is associated with endodontically-treated teeth (ETT). The aim of the present study was to analyze clinically and radiographically, at different intervals of time, some of the risk factors present in ETT that developed VRF.
Methods: A classification model according to time measured the follow-up period of three groups.