β-Carotene production from sugarcane molasses by a newly isolated Rhodotorula toruloides L/24-26-1.

Production of carotenoids by yeast fermentation is an advantaged technology due to its easy scaling and safety. Nevertheless, carotenoid production needs an economic culture medium and other efficient yeast stains. The study aims to isolate and identify a yeast strain capable of producing carotenoids using a cost-effective substrate.

Analysis of Adeno-Associated Virus Serotype 8 (AAV8)-antibody complexes using epitope mapping by molecular imprinting leads to the identification of Fab peptides that potentially evade AAV8 neutralisation.

Gene therapy is a promising approach for treating genetic disorders by delivering therapeutic genes to replace or correct malfunctioning genes. However, the introduced gene therapy vector can trigger an immune response, leading to reduced efficacy and potential harm to the patient. To improve the efficiency and safety of gene therapy, preventing the immune response to the vector is crucial.

Molecular imprinting as a tool for determining molecular markers: a lung cancer case.

Determining which cancer patients will be sensitive to a given therapy is essential for personalised medicine. Thus, it is important to develop new tools that will allow us to stratify patients according to their predicted response to treatment. The aim of work presented here was to use molecular imprinting for determining the sensitivity of lung cancer cell lines to ionising radiation based on cell surface proteomic differences.

Exploration of multifunctional properties of garlic skin derived cellulose nanocrystals and extracts incorporated chitosan biocomposite films for active packaging application.

For many years, garlic has been used as a condiment in food and traditional medicine. However, the garlic skin, which accounts for 25% of the garlic bulk, is considered agricultural waste. In this study, cellulose nanocrystals (CNCs) and garlic extract (GE) from garlic skin were isolated and used as fillers to manufacture biocomposite films.

Iodo Silanes as Superior Substrates for the Solid Phase Synthesis of Molecularly Imprinted Polymer Nanoparticles.

Current state-of-the-art techniques for the solid phase synthesis of molecularly imprinted polymer (MIP) nanoparticles typically rely on amino silanes for the immobilisation of template molecules prior to polymerisation. An investigation into commonly used amino silanes identified a number of problematic side reactions which negatively affect the purity and affinity of these polymers. Iodo silanes are presented as a superior alternative in a case study describing the synthesis of MIPs against epitopes of a common cancer biomarker, epidermal growth factor receptor (EGFR).

Selective Impedimetric Chemosensing of Carcinogenic Heterocyclic Aromatic Amine in Pork by dsDNA-Mimicking Molecularly Imprinted Polymer Film-Coated Electrodes.

Inspired by the easy intercalation of quinoxaline heterocyclic aromatic amines (HAAs) in double-stranded DNA (dsDNA), we synthesized a nucleobase-functionalized molecularly imprinted polymer (MIP) as the recognition unit of an impedimetric chemosensor for the selective determination of a 2-amino-3,7,8-trimethyl-3-imidazo[4,5-]quinoxaline () HAA. HAAs are generated in meat and fish processed at high temperatures. They are considered to be potent hazardous carcinogens.

Generic sensor platform based on electro-responsive molecularly imprinted polymer nanoparticles (e-NanoMIPs).

The present research describes the design of robust electrochemical sensors based on electro-responsive molecularly imprinted polymer nanoparticles (e-MIPs). The e-MIPs, tagged with a redox probe, combine both recognition and reporting functions. This system replaces enzyme-mediator pairs used in traditional biosensors.

Determination of sitagliptin in human plasma using a smart electrochemical sensor based on electroactive molecularly imprinted nanoparticles.

Sitagliptin is a hypoglycaemic agent used to reduce blood sugar levels in patients with type 2 diabetes mellitus (T2DM). Real time monitoring of sitagliptin levels is crucial to prevent overdose, which might cause liver, kidney and pancreatic diseases. As an alternative solution, a sitagliptin voltammetric sensor was fabricated using artificial receptors called electroactive molecularly imprinted polymer nanoparticles (nanoMIPs).

Design and fabrication of a smart sensor using in silico epitope mapping and electro-responsive imprinted polymer nanoparticles for determination of insulin levels in human plasma.

A robust and highly specific sensor based on electroactive molecularly imprinted polymer nanoparticles (nanoMIP) was developed. The nanoMIP tagged with a redox probe, combines both recognition and reporting capabilities. The developed nanoMIP replaces enzyme-mediator pairs used in traditional biosensors thus, offering enhanced molecular recognition for insulin, improving performance in complex biological samples, and yielding high stability.

Highly selective electrochemical nanofilm sensor for detection of carcinogenic PAHs in environmental samples.

A highly sensitive sensor based on molecularly imprinted polymer film was devised for determination of polycyclic aromatic hydrocarbon (PAHs) in aquatic solutions. In this paper we report, electro-polymerisation of 4-vinyl pyridine (4VP) and target, pyrene, using cyclic voltammeter in electrolyte medium, forming the pyrene imprinted polymer. After polymerisation, the pyrene was removed from imprinted polymer using methanol to produce sensory nanofilm characterised by infrared spectrometer, optical and atomic force microscopy.

Molecularly imprinted nanoparticles-based assay (MINA) - detection of leukotrienes and insulin.

A novel molecularly imprinted polymer nanoparticle-based assay (MINA) performed in magnetic microplates was developed as an improved high-quality alternative to existing antibody-based immunoassays. MINA is a generic technology that can be adapted for biomarker detection in biological samples. Herein, we demonstrate the applicability of the MINA assay for the detection of leukotrienes and insulin in biological samples.

Bactericidal In-Vitro Effect of Zinc Ferrite Nanoparticles and the Orange Wax Extracts on Three Phytopathogen Microorganisms.

Phytopathogenic bacteria affect a wide variety of crops, causing significant economic losses. Natural biocides are the alternative to chemical methods of phytopathogens control. The goal of the present study is the evaluation of the biocidal activity of the following: 1) the extract of orange wax (EOW); 2) zinc ferrite nanoparticles (ZF-NPs); 3) the EOW adsorbed on the ZF-NPs; and 4) the EOW/ZF-NPs washed with 40% ethanol.

Identifying Resistance in Strawberry Through Disease Screening of Multiple Populations and Image Based Phenotyping.

is a highly detrimental pathogen of soil cultivated strawberry (). Breeding of Verticillium wilt resistance into commercially viable strawberry cultivars can help mitigate the impact of the disease. In this study we describe novel sources of resistance identified in multiple strawberry populations, creating a wealth of data for breeders to exploit.

Determination of Fumonisin B1 in maize using molecularly imprinted polymer nanoparticles-based assay.

Fumonisin B1 (FB1) is a carcinogenic mycotoxin produced by Fusarium species contaminating maize. At present, fumonisin determination is performed using costly and demanding chromatography techniques or immunoassays. Recently, a molecularly imprinted polymer nanoparticles (nanoMIPs) - based assay (MINA) has been developed for FB1 detection.

Molecularly Imprinted Polymers in Electrochemical and Optical Sensors.

Molecular imprinting is the process of template-induced formation of specific recognition sites in a polymer. Synthetic receptors prepared using molecular imprinting possess a unique combination of properties such as robustness, high affinity, specificity, and low-cost production, which makes them attractive alternatives to natural receptors. Improvements in polymer science and nanotechnology have contributed to enhanced performance of molecularly imprinted polymer (MIP) sensors.

Biopatterning of antibodies on poly(pyrrole)-nanowires using nanocontact printing: Surface characterization.

A highly performant patterning of antibodies using poly(pyrrole) nanowires (PPy-NWs) was devised on thermoplastic surfaces based on silane derivatives. The PPy-NWs were fabricated employing nanocontact printing and controlled chemical polymerization (nCP-CCP) on poly(ethylene terephthalate), cyclic olefin copolymer, poly(ethylene 2,6-naphthalate), and polyimide. The technique used a commercial compact disk as a template (mold) to produce nanopatterned polydimethylsiloxane stamps.

[Surgical results and monitoring of postoperative atrial myxomas].

Objective: To analyze the casuistics of left atrial myxoma with emphasis on results and follow-up.

Method: We reviewed the clinical records of patients operated in the Hospital Cardiac Myxomas South Central High Specialty (HCSAE) of PEMEX in the last 7 years, with an emphasis on results and follow-up.

Results: The analysis showed 10 patients, of whom 60% were female and 40% male, with ages from 12 to 76 years, with a mean age of 50 years.

[Association between nutritional status and physical abilities in children aged 6 to 18 years in Medellin (Colombia)].

Introduction: Nutritional disorders in childhood may cause a decline in motor abilities and increased morbidity and mortality in adulthood.

Objective: To assess the association between nutritional status and motor abilities.

Materials And Methods: A cross-sectional study was performed that included 12,872 children aged between 6 and 18 years who underwent a clinical evaluation and various physical tests.

Effect of imiquimod as compared with surgery on the cancerization field in basal cell carcinoma.

Background: Patients with basal cell carcinoma (BCC) have an increased risk of subsequent BCCs. It is possible that imiquimod might reduce this risk by acting on the cancerization field.

Objective: To examine the ability of imiquimod to reduce subsequent BCCs.

[Evaluation of a rapid immunological test for tuberculosis diagnosis].

Objective: To demonstrate whether patients undergoing capsulotomy Nd: YAG laser developed intraocular hypertension after the procedure.

Methods: Prospective, pre-experimental before and after 2 measurements with post-test in patients with posterior capsular opacity from the Ophthalmology Service. Measurements of intraocular pressure (IP) before capsulotomy Nd: YAG laser, and three hours and one week later.

[Who funds research by Spanish dermatologists? Comparative analysis of articles published in 2008].

Background: References have been made in the literature to the funding of clinical trials by the pharmaceutical industry. Other types of funding, however, have been less well studied.

Objective: To describe the sources of funding for research by Spanish dermatology departments published in 2008.