Heterologous Prime-Boost Immunization Strategies Using Varicella-Zoster Virus gE mRNA Vaccine and Adjuvanted Protein Subunit Vaccine Triggered Superior Cell Immune Response in Middle-Aged Mice.

Purpose: Heterologous immunization using different vaccine platforms has been demonstrated as an efficient strategy to enhance antigen-specific immune responses. In this study, we performed a head-to-head comparison of both humoral and cellular immune response induced by different prime-boost immunization regimens of mRNA vaccine and adjuvanted protein subunit vaccine against varicella-zoster virus (VZV) in middle-aged mice, aiming to get a better understanding of the influence of vaccination schedule on immune response.

Methods: VZV glycoprotein (gE) mRNA was synthesized and encapsulated into SM-102-based lipid nanoparticles (LNPs).

Enhanced Therapeutic Potential of Hybrid Exosomes Loaded with Paclitaxel for Cancer Therapy.

The advancement of exosome studies has positioned engineered exosomes as crucial biomaterials for the development of advanced drug delivery systems. This study focuses on developing a hybrid exosome system by fusing mesenchymal stem cells (MSCs) exosomes with folate-targeted liposomes. The aim was to improve the drug loading capacity and target modification of exosome nanocarriers for delivering the first-line chemotherapy drug paclitaxel (PTX) and its effectiveness was assessed through cellular uptake studies to evaluate its ability to deliver drugs to tumor cells in vitro.

Effects of pork sausage on intestinal microecology and metabolism in mice.

Background: Processed meat, as an important part of the human diet, has been recognized as a carcinogen by the International Agency for Research on Cancer (IARC). Although numerous epidemiological reports supported the IARC's view, the relevant evidence of a direct association between processed meat and carcinogenicity has been insufficient and the mechanism has been unclear. This study aims to investigate the effects of pork sausage (as a representative example of processed meat) intake on gut microbial communities and metabolites of mice.

Reduction of Ultrafiltration Membrane Fouling by the Pretreatment Removal of Emerging Pollutants: A Review.

Ultrafiltration (UF) processes exhibit high removal efficiencies for suspended solids and organic macromolecules, while UF membrane fouling is the biggest obstacle affecting the wide application of UF technology. To solve this problem, various pretreatment measures, including coagulation, adsorption, and advanced oxidation, for application prior to UF processes have been proposed and applied in actual water treatment processes. Previously, researchers mainly focused on the contribution of natural macromolecular pollutants to UF membrane fouling, while the mechanisms of the influence of emerging pollutants (EPs) in UF processes (such as antibiotics, microplastics, antibiotic resistance genes, etc.

Simultaneous separation of glycyrrhizic acid, baicalein and wogonin from Radix Glycyrrhizae and Radix Scutellariae using foam fractionation and in vitro activity evaluation.

Background: In this study, the optimal conditions for the extraction and purification of glycyrrhizic acid from Radix Glycyrrhizae (RG) and baicalein and wogonin from Radix Scutellariae (RS) by foam fractionation were studied on the basis of central composite design (CCD) and response surface methodology.

Results: The results showed that herbal proportion (RG:RS), gas flow and ethanol concentration were the main factors guiding the foam fractionation of RG and RS. The optimum technological parameters were obtained as follows: herbal proportion (RG:RS), 1.

Mg/Al-LDH as a nano-adjuvant for pertussis vaccine: a evaluation compared with aluminum hydroxide adjuvant.

. Layered double hydroxide (LDH) has been demonstrated as a highly efficient antigen platform to induce effective and durable immune response. However, whether LDH nanoparticles could act as an adjuvant for pertussis vaccines is still unknown.

Alteration in concentration-response curves of four N-alkylpyridinium chloride by exposure concentration, time and in their mixtures by uniform design.

The concentration-response curves (CRCs) of chemicals are important in extrapolating their effects from laboratory studies to their risk assessment in the field. Yet, the CRCs can be altered by exposure concentration and mixture conditions, and also by exposure time in recent reports. Presently, ionic liquids (N-alkylpyridinium chloride, [apyr]Cl) were used for CRC-alteration studies.