To enhance the gel properties of Alaskan pollock surimi, the Acetes chinensis powder (ACP) with different contents (0.5-3 % w/w) was added to the surimi and its mechanisms were investigated. Results showed that adding 1.
View Article and Find Full Text PDFThe objective of this study was to examine the impact of magnetic field-assisted thawing (MFT) on the quality and microstructure of Portunus trituberculatus meat, in comparison to other thawing methods (Air thawing, AT; Hydrostatic thawing, HT; Microwave thawing, MT). A comprehensive comparative analysis was conducted on different physicochemical and flavor properties of crab meat. MFT significantly reduced thawing loss by 18.
View Article and Find Full Text PDFPhys Chem Chem Phys
October 2024
To gain insights into the light-harvesting capabilities of the chromophores, it is essential to understand their molecular and electronic structures within their natural chemical or biological contexts. Rhodopsins display varied absorption characteristics due to the interaction between the chromophore retinal and its surrounding protein environments. In this study, we employed a quantum mechanics/molecular mechanics approach to examine a series of artificially designed rhodopsin mimics based on human cellular retinol acid binding protein 2 (hCRABP II).
View Article and Find Full Text PDFTo better enhance printing effects meanwhile casting functionality, antioxidation and absorption of bioactive component in printed Ca-nano starch (NS)-lutein (L)-surimi were investigated. Results shown that Ca-NS-L promoted surimi printability due to enhanced gel strength and denser structure. Mixing Ca-NS-L endowed printed surimi with antioxidation (DPPH, ABTS, hydroxyl radical, Fe reduction were 42 %, 79 %, 65 %, 0.
View Article and Find Full Text PDFFunctional compounds (FCs) had some functions, which are affected easily by digestion and transmembrane transport leading to low absorption rates, such as lutein, quercetin, xylo-oligosaccharide. Protein from blue foods is a potential bioactive compound, which had higher bioavailability, especially for bioactive peptides (BBPs). The BBPs has great limitations, especially the variability under pepsin digestion.
View Article and Find Full Text PDFBackground: Giant salamander protein peptide is a peptide with rich functional properties. Giant salamander protein peptide KGEYNK (KK-6) is a peptide with both antioxidant and anti-inflammatory properties. The antioxidant and anti-inflammatory mechanisms of KK-6 are still unclear.
View Article and Find Full Text PDFMicrobial rhodopsin, a pivotal photoreceptor protein, has garnered widespread application in diverse fields such as optogenetics, biotechnology, biodevices, etc. However, current microbial rhodopsins are all transmembrane proteins, which both complicates the investigation on the photoreaction mechanism and limits their further applications. Therefore, a specific mimic for microbial rhodopsin can not only provide a better model for understanding the mechanism but also can extend the applications.
View Article and Find Full Text PDFA novel stable PVA/HPMC/roselle anthocyanin (RAE) indicator film co-pigmented with oxalic acid (OA) was prepared, its properties, application effects and stability enhancement mechanism were investigated correspondingly. The structural characterization revealed that more stable network was formed due to the co-pigmentation facilitated generation of molecular interactions. Meanwhile, the co-pigmentation improved film mechanical and hydrophobic properties compared to both PVA/HPMC/RAE newly prepared (PHRN) or stored (PHRS) film, expressing as higher tensile strength values (12.
View Article and Find Full Text PDFIn order to investigate the influences of modified RAE-based film on shrimp quality, the proteomic approach was performed to elucidate preservation mechanism. Results showed that the modified RAE-based film kept better shrimp quality compared with natural RAE-based film in terms of determined biochemical parameters and estimated shelf-life. Totally, 49 differentially abundance proteins (DAPs) were identified compared with shrimp without packaging.
View Article and Find Full Text PDFTo solve undiscernible freshness changes of printed functional surimi while maintaining printed shape, 4D printable color-changing material were prepared. Firstly, based on results of printing properties and fresh-keeping of Ca-NS-L-surimi, it showed better printing effects (enhanced mechanical strength) and good preservation (inhibition of amino acids decomposition, bacterial growth). However, freshness changes of printed Ca-NS-L-surimi were not distinguished directly.
View Article and Find Full Text PDFBackground: Nano starch-lutein (NS-L) can be used in three-dimensional (3D) printed functional surimi. However, the lutein release and printing effect are not ideal. The purpose of this study was to facilitate the function and printing properties of surimi by adding the combination of calcium ion (Ca ) and NS-L.
View Article and Find Full Text PDFBiological lasers have attracted vast attention because of their potential medical application prospects, especially the low threshold biological laser that can be used for ultrasensitive biological detection while leaving the luminous gain medium undamaged by the pump light. By coupling the low concentration green fluorescent protein (GFP) solution with a high Q whispering gallery mode microbubble resonator, we managed to fabricate a miniature GFP laser with the lowest threshold and highest Q value compared to any known type of the GFP laser. The threshold energy is as low as 380 fJ, two orders of magnitude lower than any type of GFP laser at present.
View Article and Find Full Text PDFThe rhodopsin mimic is a chemically synthetized complex with retinyl Schiff base (RSB) formed between protein and the retinal chromophore that can mimic the natural rhodopsin-like protein. The artificial rhodopsin mimic is more stable and designable than the natural protein and hence has wider uses in photon detection devices. The mimic structure RSB, like the case in the actual rhodopsin-like protein, undergoes isomerization and protonation throughout the photoreaction process.
View Article and Find Full Text PDFMislabeling and adulteration of tuna are common due to the diminishing of morphological characteristics during processing. The tuna authenticity is now being focused in the seafood supply chain. In this study, the lipid profiles of 3 commercial tuna species (skipjack tuna, bigeye tuna and yellowfin tuna) were investigated via ultra-high performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap MS).
View Article and Find Full Text PDFWe separated a novel functional peptide IFPPKPKDTL from porcine plasma hydrolysate by chromatography, HPLC, and identified by Q Exactive LC-MS/MS. Results showed that IFPPKPKDTL had a significant ability of ACE inhibition (76.6%) likely due to the presence of hydrophobic, aromatic, and acidic amino acids that can inactivate ACE by binding Zn, providing a hydrogen atom to maintain the link between ACE and the peptide.
View Article and Find Full Text PDFBackground: Pig plasma contains a large amount of protein. Porcine plasma polypeptide can be prepared by the enzymatic hydrolysis of porcine plasma protein. The present study investigated the function, structure, and mechanisms of porcine plasma peptides.
View Article and Find Full Text PDFThe function of chitosan film was reinforced by ZnO nanoparticles and antioxidant of bamboo leaves (AOB) for food packaging application. The results of structural characterization indicated the good compatibility among chitosan, ZnO nanoparticles and AOB. The chitosan film had the best mechanical strength and the highest light transmittance.
View Article and Find Full Text PDFA novel antioxidant peptide EDEQKFWGK from porcine plasma hydrolysate (PPH) was separated by chromatography, HPLC, and identified by LC-MS/MS. Results showed that EDEQKFWGK had better antioxidant ability (Hydroxyl RAS 32.19%, ABTS RAS 92.
View Article and Find Full Text PDFThe increased interest in achieving, solely through diet, the same effect on iron levels with supplementation, leads to numerous studies on iron absorption of iron binding proteins (IBPs). The characteristics of IBPs from Tegillarca granosa (T. granosa) and its iron utilization were determined to analyze their relationship.
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