Publications by authors named "Gaopo Xu"
Background: Immunotherapy is beneficial for some colorectal cancer (CRC) patients, but immunosuppressive networks limit its effectiveness. Cancer-associatedfibroblasts (CAFs) are significant in immune escape and resistance toimmunotherapy, emphasizing the urgent need for new treatment strategies.
Methods: Flow cytometric, Western blotting, proteomics analysis, analysis of public database data, genetically modified cell line models, T cell coculture, crystal violetstaining, ELISA, metabonomic and clinical tumour samples were conducted to assess the role of EDEM3 in immune escape and itsmolecular mechanisms.
Article Synopsis
- * Increased G4 formations in colorectal cancer cells are linked to more CD8 T cell infiltration, and the G4 ligand TMPyP4 has been shown to hinder cancer growth and stimulate immune responses.
- * TMPyP4 not only stops cancer cell division but also boosts anti-tumor immunity by activating the cGAS-STING pathway, and its combination with anti-PD1 therapy shows enhanced effects against colorectal cancer.
Background: Aging confers an increased risk of developing cancer, and the global burden of cancer is cumulating as human longevity increases. Providing adequate care for old patients with rectal cancer is challenging and complex.
Method: A total of 428 and 44,788 patients diagnosed with non-metastatic rectal cancer from a referral tertiary care center (SYSU cohort) and the Surveillance Epidemiology and End Results database (SEER cohort) were included.
Article Synopsis
- N6-methyladenosine (m6A) modification is identified as a key epigenetic mechanism influencing tumor development, specifically involving the enzyme ALKBH5, which demethylates m6A.
- Research revealed that ALKBH5 is overexpressed in colorectal cancer (CRC) tissues, correlating with poorer patient survival and promoting cell growth and aggression in CRC.
- The study found that ALKBH5 enhances RAB5A expression through demethylation, preventing its degradation, suggesting the ALKBH5-RAB5A pathway could be critical for CRC progression and a potential target for treatment.
Background: More than ten randomized clinical trials are being tested to evaluate the efficacy, effectiveness and safety of a fasting-mimicking diet (FMD) combined with different antitumor agents.
Methods: UMI-mRNA sequencing, Cell-cycle analysis, Label retention, metabolomics, Multilabeling et al. were used to explore mechanisms.
Article Synopsis
- * Findings showed that patients with negative baseline CEA levels had significantly lower sensitivity in detecting recurrences compared to those with elevated baseline levels, but both groups had similar specificity.
- * Adding CA19-9 to CEA testing improved sensitivity for those with negative baseline CEA, indicating that more comprehensive surveillance methods could enhance patient outcomes in detecting cancer recurrence.
Background: Systemic inflammation status has been recognized as a sensitive marker associated with survival in cancers and anti-inflammatory treatment outcomes in inflammation-derived diseases. This study aimed to investigate the role of systemic inflammation status as a predictive marker for survival and anti-inflammatory treatment benefit in rectal cancer patients.
Methods: A total of 475 patients with stage I-III rectal cancer receiving curative resection were prospectively enrolled.
Nomograms for Prediction of Molecular Phenotypes in Colorectal Cancer.
Onco Targets Ther
January 2020
Background: Colorectal cancer (CRC) patients with different molecular phenotypes, including microsatellite instability (MSI), CpG island methylator phenotype (CIMP), and somatic mutations in and gene, vary in treatment response and prognosis. However, molecular phenotyping under adequate quality control in a community-based setting may be difficult. We aimed to build the nomograms based on easily accessible clinicopathological characteristics to predict molecular phenotypes.
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- Drug-resistant Mycobacterium tuberculosis has become a major public health issue, complicating tuberculosis treatment, and the study explores the molecular mechanisms behind levofloxacin resistance.
- Through transcriptome and methylome sequencing, the research identified significant changes in gene expression and DNA methylation associated with levofloxacin resistance, revealing 953 differentially expressed genes and 239 differentially methylated genes.
- Key overlapping genes were discovered that could play crucial roles in the resistance mechanisms, particularly genes like cyp132, pckA, and pfkB, which may help inform future TB treatment strategies.
This study focused on AflSkn7, which is a stress response regulator in the aflatoxin-producing Aspergillus flavus. The ΔAflSkn7 mutants exhibited partially defective conidial formation and a complete inability to generate sclerotia, indicating AflSkn7 affects A. flavus asexual and sexual development.
View Article and Find Full Text PDFInhibition of aflatoxin metabolism and growth of Aspergillus flavus in liquid culture by a DNA methylation inhibitor.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
December 2015
Aflatoxins (AFs) are a group of highly oxygenated polyketidese-derived toxins mainly produced by Aspergillus flavus and A. parasiticus, whose biosynthesis mechanisms are extremely sophisticated. Methylation is known as the major form of epigenetic regulation, which is correlated with gene expression.
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