Isogeometric boundary element method analysis for dielectric target shape optimization in electromagnetic scattering.

In order to optimize the overall form of electromagnetic scattering in two-dimensional dielectric media, this work offers a frequency-domain boundary element method based on isogeometric analysis. The Isogeometric boundary element method (IGABEM) is used to guarantee geometric correctness during optimization and prevent over-refinement of the mesh. Non-uniform rational B-splines are used to discretize the boundary integrals of the model, enabling rapid numerical computation while ensuring high accuracy.

Quenching and Electrical Current Poling Effect on Improved Ferroelectric and Piezoelectric Properties in BiFeO-Based High-Temperature Piezoceramics.

The effects of quenching on the structural, electrical, dielectric, ferroelectric (FE), and piezoelectric properties are investigated systematically in the 0.85BiFeCrO-0.15BaTiMnO (0 ≤ ≤ 0.

Identification of a metagenome-derived prephenate dehydrogenase gene from an alkaline-polluted soil microorganism.

A novel prephenate dehydrogenase gene designated pdhE-1 was cloned by sequence-based screening of a plasmid metagenomic library from uncultured alkaline-polluted microorganisms. The deduced amino acid sequence comparison and phylogenetic analysis indicated that PdhE-1 and other putative prephenate dehydrogenases were closely related. The putative prephenate dehydrogenase gene was subcloned into pETBlue-2 vector and overexpressed in Escherichia coli BL21(DE3) pLacI.

[Evaluation of norovirus rapid detection kit].

Objective: Evaluating the accuracy and safety as well as the equivalence compared with the control kit of RIDA QUICK Norovirus detection kit(R-Biopharm, Germany).

Methods: Based on the results of commercially available IDEA Norovirus detection kit (ELISA), the sensitivity and specificity and accuracy of RIDA QUICK Norovirus detection kit (immunochromatographic assay) were evaluated.

Results: The sensitivity and specificity of RIDA QUICK Norovirus detection kit were 98.

Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms.

Background: Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L-malate from the substrate fumarate. Thermostable and high-activity fumarases from organisms that inhabit extreme environments may have great potential in industry, biotechnology, and basic research.

Biochemical characterization of two novel β-glucosidase genes by metagenome expression cloning.

Two novel β-glucosidase genes designated as bgl1D and bgl1E, which encode 172- and 151-aa peptides, respectively, were cloned by function-based screening of a metagenomic library from uncultured soil microorganisms. Sequence analyses indicated that Bgl1D and Bgl1E exhibited lower similarities with some putative β-glucosidases. Functional characterization through high-performance liquid chromatography demonstrated that purified recombinant Bgl1D and Bgl1E proteins hydrolyzed D-glucosyl-β-(1-4)-D-glucose to glucose.

[The analysis of the test results in HIV screening laboratory of Beijing Friendship Hospital in 2008].

Objective: According to test results of the Hospital of AIDS screening laboratory in 2008, after counting analysis to assess the prevalence of AIDS, we can early detect positive cases in the future and effectively control the spread of AIDS.

Methods: All serum samples were screened by ELISA method and we reexaminated the samples by PA. As long as one result is positive by the two methods, then we sent the positive samples to Beijing Center for Disease Control and Prevention by Western Blot method to confirm the result.

[In vitro inhibitory mechanism of respiratory syncytial virus with solution prescription of zhidanhuayu].

Objective: To study the effective part of solution prescription of Zhidanhuayu (ZDHY) against respiratory syncytial virus (RSV) in vitro.

Methods: Observe the pathology of RSV to Hep-2 under the condition of different concentrations and each effective part of ZDHY.

Results: The concentration limit causing celluar toxicity of ZDHY is 5.

[In vitro inhibition of respiratory syncytial virus with combined ribavirin and an oral preparation of traditional Chinese medicine Hu Fei].

Objective: To study the in vitro antiviral effect of ribavirin combined with an oral preparation of traditional Chinese medicine "Hu Fei" (protecting the lung) on respiratory syncytial virus (RSV).

Methods: Cytopathic effects (CEP) of RSV on Hep2 cells were observed after adding different concentrations of ribavirin, Hu Fei and combination of both into the culture medium.

Results: The minimum concentration of ribavirin and Hu Fei for complete inhibition of CPE caused by RSV was 7.