Publications by authors named "Gao-Miao Zhan"
Article Synopsis
- The cosuppression of the Rubisco small subunit gene RBCS3B in Arabidopsis led to albino or pale green phenotypes primarily due to the accumulation of reactive oxygen species (ROS).
- Four transgenic lines (RBCS3B-7, 18, 33, and 35) were created, with RBCS3B-7 showing the most severe developmental issues, including reduced height and plant mortality before flowering.
- RBCS3B-7 experienced compromised chloroplast function, with decreased chloroplast numbers and broken thylakoids, resulting in severe photoinhibition caused by ROS accumulation, which damaged the photosystem II (PSII) and hindered its repair.
Seed yield and oil content are two important agricultural characteristics in oil crop breeding, and a lot of functional gene research is being concentrated on increasing these factors. In this study, by differential gene expression analyses between rapeseed lines (zy036 and 51070) which exhibit different levels of seed oil production, BnGRF2 (Brassica napus growth-regulating factor 2-like gene) was identified in the high oil-producing line zy036. To elucidate the possible roles of BnGRF2 in seed oil production, the cDNA sequences of the rapeseed GRF2 gene were isolated.
View Article and Find Full Text PDFSeed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content.
View Article and Find Full Text PDFPyruvate dehydrogenase kinase (PDK) is a negative regulator of the mitochondrial pyruvate dehydrogenase complex (mtPDC), which plays a key role in intermediary metabolism. In this study, a 1,490-bp PDK in Brassica napus (BnPDK1) was isolated and cloned from Brassica cDNA library. BnPDK1 has an 1,104 open reading frame encoding 367 amino acids.
View Article and Find Full Text PDF