[Transcriptome profiling of high oleic peanut under low temperatureduring germination].

High oleic (HO) peanut (Arachishypogaea L.) oils benefit human health and industrial production due to its superior nutritional composition and thermo-oxidative stability. However, HO peanut is sensitive to cold stress especially during germination, which limits its distribution in low temperature areas.

[Isolation of the promoter region of HAK gene from Aeluropus littoralis and functional analysis in rice].

The AlHAK1 gene encoding a high-affinity K+ transporter was isolated from Aeluropus littoralis (Gouan) Parl, a graminaceous halophyte, and plays a crucial role in nutrition and ion homeostasis in plant cell. To investigate the regulation role of AlHAK1 on the transcriptional level, an about 1.3 kb 5'-flanking region of the AlHAK1 gene containing a putative promoter was cloned by genome walking method.

Identification of ATP synthase beta subunit (ATPB) on the cell surface as a non-small cell lung cancer (NSCLC) associated antigen.

Background: Antibody-based immunotherapy has achieved some success for cancer. But the main problem is that only a few tumor-associated antigens or therapeutic targets have been known to us so far. It is essential to identify more immunogenic antigens (especially cellular membrane markers) for tumor diagnosis and therapy.

[Differentiation of erythroleukemia K562 cells induced by piperine].

Background & Objective: The most common haematological malignancy is leukaemia. Differentiation induction is considered as one of the effective therapies for leukemia. Piperine, an alkaloid extracted from piperaceae, has been reported to display a variety of pharmacological activities, including sedation, anti-inflammation and antitumor effects.

Characterization and expression of a vacuolar Na(+)/H(+) antiporter gene from the monocot halophyte Aeluropus littoralis.

Plant vacuolar Na(+)/H(+) antiporter plays an important role in salt tolerance. In order to understand the molecular basis of vacuolar Na(+)/H(+) antiporter responded to salinity and reveal a possible role of salt tolerance in monocots, a vacuolar Na(+)/H(+) antiporter gene (AlNHX) was isolated by reverse transcription-PCR and RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE) based on the homology from Aeluropus littoralis (Gouan) Parl, a graminaceous halophyte. The AlNHX sequence contained 2706bp with an open read frame of 1623bp and the deduced transcripts encoding 540 amino acids shared a high homology with those putative vacuolar Na(+)/H(+) antiporters of higher plants.

Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light.

Aim: To investigate the apoptotic effect of photoexcited titanium dioxide (TiO(2)) nanoparticles in the presence of visible light on human hepatoma cell line (Bel 7402) and to study the underlying mechanism.

Methods: Cerium-element-doped titanium dioxide nanoparticles were prepared by impregnation method. Bel 7402 human hepatoma cells were cultured in RPMI 1640 medium in a humidified incubator with 50 mL/L CO(2) at 37 centigrade.

Observation of DNA damage of human hepatoma cells irradiated by heavy ions using comet assay.

Aim: Now many countries have developed cancer therapy with heavy ions, especially in GSI (Gesellschaft f r Schwerionenforschung mbH, Darmstadt, Germany), remarkable results have obtained, but due to the complexity of particle track structure, the basic theory still needs further researching. In this paper, the genotoxic effects of heavy ions irradiation on SMMC-7721 cells were measured using the single cell gel electrophoresis (comet assay). The information about the DNA damage made by other radiations such as X-ray, gamma-ray, UV and fast neutron irradiation is very plentiful, while little work have been done on the heavy ions so far.