Software-aided efficient identification of the components of compound formulae and their metabolites in rats by UHPLC/IM-QTOF-MS and an in-house high-definition MS library: Sishen formula as a case.

Identifying the compound formulae-related xenobiotics in bio-samples is full of challenges. Conventional strategies always exhibit the insufficiencies in overall coverage, analytical efficiency, and degree of automation, and the results highly rely on the personal knowledge and experience. The goal of this work was to establish a software-aided approach, by integrating ultra-high performance liquid chromatography/ion-mobility quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) and in-house high-definition MS library, to enhance the identification of prototypes and metabolites of the compound formulae , taking Sishen formula (SSF) as a template.

The Role of Gut Microbiome in Irritable Bowel Syndrome: Implications for Clinical Therapeutics.

Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder (FGID) characterized by chronic or recurrent gastrointestinal symptoms without organic changes, and it is also a common disorder of gut-brain interaction (DGBIs).. The symptoms of IBS not only affect the quality of life for individual patients but also place a significant burden on global healthcare systems.

Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer.

Introduction: Triple-negative breast cancer (TNBC) has a high mortality rate and limited treatment options. Tetrahydrocurcumin (THC), a major metabolite of curcumin, has potential antitumor activities. However, the antitumor effects and mechanism of THC in TNBC remain elusive.

Circulating metabolites of Borneolum syntheticum (Bingpian) ameliorate atherosclerosis in ApoE mice via inhibiting macrophage foam-cell formation.

Translational pharmacological research on traditional medicines lays the foundation for precisely understanding how the medicines function in the body to deliver therapeutic benefits. Borneolum syntheticum (Bingpian) is commonly used in Chinese herbal medicines for coronary heart disease, but its specific cardiovascular impact remains poorly understood. Isoborneol, a constituent of Bingpian, has been found to reduce lipid accumulation in macrophages in vitro, but its oral bioavailability is limited.

Difference between real world practice and clinical research: A comparison of oropharyngeal and nasopharyngeal sampling data in influenza.

Oropharyngeal (OP) sampling is one of the most commonly used methods for respiratory sampling, but its positivity rate in real practice compared with that of nasopharyngeal (NP) sampling is not fully known. The differences between OP and NP in practice and between practice and the literature were compared. In total, 2323 positive results from 6708 patients were recorded in the laboratory test system, and 15,021 positive results from 31,333 patients were recorded in the national report.

Perilla frutescens leaf extracts alleviate acute lung injury in mice by inhibiting KAT2A.

Ethnopharmacological Relevance: Acute lung injury (ALI) can lead to respiratory failure and even death. KAT2A is a key target to suppress the development of inflammation. A herb, perilla frutescens, is an effective treatment for pulmonary inflammatory diseases with anti-inflammatory effects; however, its mechanism of action remains unclear.

Benefit delayed immunosenescence by regulating CD4T cells: A promising therapeutic target for aging-related diseases.

CD4T cells play a notable role in immune protection at different stages of life. During aging, the interaction between the body's internal and external environment and CD4T cells results in a series of changes in the CD4T cells pool making it involved in immunosenescence. Many studies have extensively examined the subsets and functionality of CD4T cells within the immune system, highlighted their pivotal role in disease pathogenesis, progression, and therapeutic interventions.

SENP1-Mediated HSP90ab1 DeSUMOylation in Cardiomyocytes Prevents Myocardial Fibrosis by Paracrine Signaling.

Myocardial infarction (MI) triggers a poor ventricular remodeling response, but the underlying mechanisms remain unclear. Here, the authors show that sentrin-specific protease 1 (SENP1) is downregulated in post-MI mice and in patients with severe heart failure. By generating cardiomyocyte-specific SENP1 knockout and overexpression mice to assess cardiac function and ventricular remodeling responses under physiological and pathological conditions.

Effect of hyperoside on osteoporosis in ovariectomized mice through estrogen receptor α/ITGβ3 signaling pathway.

Osteoporosis is a highly prevalent bone metabolic disease in menopause due to estrogen deficiency. Hyperoside is a main compound in Semen cuscutae. Our team previously reported that Semen cuscutae has anti osteoporosis effect on ovariectomized mice by inhibiting bone resorption of osteoclasts.

Effects of Multidimensional Carbon-Based Nanomaterials on the Low-Carbon and High-Performance Cementitious Composites: A Critical Review.

Cementitious composites are ubiquitous in construction, and more and more research is focused on improving mechanical properties and environmental effects. However, the jury is still out on which material can achieve low-carbon and high-performance cementitious composites. This article compares the mechanical and environmental performance of zero-dimensional fullerenes, one-dimensional carbon nanotubes (CNTs), two-dimensional graphene oxide (GO), and three-dimensional nano-graphite platelets (NGPs) on cementitious composites.

ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway.

Sepsis is a life-threatening multiple organ dysfunction syndrome (MODS) caused by a microbial infection that leads to high morbidity and mortality worldwide. Sepsis-induced cardiomyopathy (SIC) and coagulopathy promote the progression of adverse outcomes in sepsis. Here, we reported that ACT001, a modified compound of parthenolide, improved the survival of sepsis mice.

Fingerprinting and characterization of the polysaccharides from Polygonatum odoratum and the in vitro fermented effects on Lactobacillus johnsonii.

Polygonatum odoratum (Yu-Zhu) can be utilized to treat the digestive and respiratory illness. Previous studies have revealed that the underlying therapeutic mechanism of P. odoratum polysaccharides (POPs) is associated with remodeling the gut microbiota.

Phenolic acids from Prunella vulgaris alleviate cardiac remodeling following myocardial infarction partially by suppressing NLRP3 activation.

Acute myocardial infarction (MI) is one of the leading causes of mortality around the world. Prunella vulgaris (Xia-Ku-Cao in Chinese) is used in traditional Chinese medicine practice for the treatment of cardiovascular diseases. However, its active ingredients and mechanisms of action on cardiac remodeling following MI remain unknown.

polysaccharide attenuates lipopolysaccharide-induced lung injury in mice by regulating gut microbiota.

is appreciated for its edible and medicinal benefits especially for lung protection. However, the contained active components have been understudied, and further research is required to fully exploit its potential application. We aimed to probe into the beneficial effects of polysaccharide (POP) in lipopolysaccharide-induced lung inflammatory injury mice.

A metabolomics discrimination-based strategy for screening the antithrombin active markers of perilla seeds: A natural oil crop.

Natural crops oil with high nutritional value has gradually attracted attention. Perilla seeds are regarded as a source of functional edible oil in America, Asia and European countries due to its abundant nutrients. In this research, samples were extracted by different polarity solvents and evaluated their thrombin inhibition activities in vitro.

RT-Ensemble Pred: A tool for retention time prediction of metabolites on different LC-MS systems.

Liquid chromatography-mass spectrometry (LC-MS) could provide a large amount of information to assist in metabolites identification. Different liquid chromatographic methods (CMs) could produce different retention times to the same metabolite. To predict the retention time of local dataset by online datasets has become a trend, but the datasets downloaded from different databases were differences in quantity levels.

An efficient approach addressing the chemical complexity of Jiawei Fangji Huangqi decoction by integrating ultra-high-performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry and intelligent data processing workflows.

A challenge in the quality control of traditional Chinese medicine is the systematic multicomponent characterization of the compound formulae. Jiawei Fangji Huangqi, a modified form of Fangji Huangqi, is a prescription comprising seven herbal medicines. To address the chemical complexity of the Jiawei Fangji Huangqi decoction, we integrated ion mobility-quadrupole time-of-flight high-definition MS coupled to ultra-high-performance liquid chromatography and intelligent data processing workflows available in the UNIFI software package.