Cross-Species Epitope Sequence Analysis for Discovery of Existing Antibodies Useful for Phospho-Specific Protein Detection in Model Species.

Signaling pathways play key roles in many important biological processes, such as cell division, differentiation, and migration. Phosphorylation site-specific antibodies specifically target proteins phosphorylated on a given tyrosine, threonine, or serine residue. The use of phospho-specific antibodies facilitates the analysis of signaling pathway regulation and activity.

Cis-regulation of the CYP6CS1 gene and its role in mediating cross-resistance in a pymetrozine-resistant strain of Nilaparvata lugens.

Pymetrozine is currently one of the primary insecticides used to control the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), but the long-term effectiveness of this chemical is threatened by growing issues of resistance. Previous studies in a laboratory selected strain of N. lugens, Pym-R, have shown that resistance to pymetrozine can evolve without target-site mutations.

Dietary fermented mixed ingredient product enhances growth performance and intestinal stem cell-mediated epithelial regeneration through Wnt/β-catenin pathway in layer chicks.

This study aimed to investigate the effects of dietary supplements of fermented mixed ingredient product (FMIP) on the growth performance, intestinal health, and immune performance of layer hens during the brooding period. Four hundred eighty healthy one-day-old layer chicks were randomly divided into four groups (six replicates/group, twenty hens/replicate) and were fed with different experimental diets for eight weeks (from day 1 to day 56): (1) Corn-soybean-base diet (CON); (2) Chlortetracycline group (CTC; CON diet supplemented with 0.5g/kg chlortetracycline); (3) 4 % fermented mixed ingredient product (4 % FMIP); (4) 8 % fermented mixed ingredient product (8 % FMIP).

Effects of Low-Severity Fire on the Composition and Stability of Soil Organic Carbon in Permafrost Peatlands (Northeast China).

Climate change and human activity are increasing the frequency of wildfires in peatlands and threatening permafrost peatland carbon pools. In Northeast China, low-severity prescribed fires are conducted annually on permafrost peatlands to reduce the risk of wildfires. These fires typically do not burn surface peat but lead to the loss of surface vegetation and introduction of pyrogenic carbon.

Electrochemical Cyclopropanation of Unactivated Alkenes with Methylene Compounds.

Cyclopropanes are prevalent in natural products, pharmaceuticals, and bioactive compounds, functioning as a significant structural motif. Although a series of methods have been developed for the construction of the cyclopropane skeleton, the development of a direct and efficient strategy for the rapid synthesis of cyclopropanes from bench-stable starting materials with a broad substrate scope and functional group tolerance remains challenging and highly desirable. Herein, we present an electrochemical method for the direct cyclopropanation of unactivated alkenes using active methylene compounds.

Cu-Functionalized, ZIF-8-Derived, Nitrogen-Doped Carbon Composites for Efficient Iodine Elimination in Solution.

The development of copper-based materials with a high efficiency and low cost is desirable for use in iodine (I) remediation. Herein, Cu-nanoparticles-functionalized, ZIF-8 (Zeolite Imidazole Framework-8)-derived, nitrogen-doped carbon composites (Cu@Zn-NC) were synthesized by ball milling and pyrolysis processes. The as-prepared composites were characterized using SEM, BET, XRD, XPS, and FT-IR analyses.

Green Synthesis of Copper Nanoparticles from the Aqueous Extract of Thunb and Evaluation of Its Catalytic Property and Cytotoxicity and Antimicrobial Activity.

In this study, copper nanoparticles with an average particle size of 2-4 nm were synthesized using the green extract of Thunb. The catalytic activity and dye degradation efficiency of Cu NPs were evaluated using ultraviolet spectroscopy. To confirm that Cu NPs can continuously remove organic dyes, this study used Cu/Lj-C composite material adsorbed on cotton balls as a simulated bed to study the cyclic catalytic activity of Cu NPs for the reduction of methylene blue by sodium borohydride (NaBH).

The Effects of Astaxanthin on Metabolic Syndrome: A Comprehensive Review.

Metabolic syndrome (MS) represents a complex cluster of metabolic disorders primarily characterized by obesity, insulin resistance, hyperglycemia, dyslipidemia, hypertension, and hyperuricemia. Diet and functional ingredients play a pivotal role in seeking non-pharmacological strategies to prevent and ameliorate MS. Astaxanthin (AST), a carotenoid found in various marine organisms, exhibits exceptional antioxidant properties and holds great promise as a natural compound that improves MS.

Synergistic Approach of High-Precision 3D Printing and Low Cell Adhesion for Enhanced Self-Assembled Spheroid Formation.

Spheroids, as three-dimensional (3D) cell aggregates, can be prepared using various methods, including hanging drops, microwells, microfluidics, magnetic manipulation, and bioreactors. However, current spheroid manufacturing techniques face challenges such as complex workflows, the need for specialized personnel, and poor batch reproducibility. In this study, we designed a support-free, 3D-printed microwell chip and developed a compatible low-cell-adhesion process.

Aerobic Copper-Catalyzed Hydroxysulfonylation of Vinylarenes with Sodium Sulfinates.

A novel and efficient method for the intermolecular hydroxysulfonylation of vinylarenes using sodium sulfinates has been achieved through aerobic copper catalysis. This transformation proceeded smoothly with green air as the terminal oxidant in the presence of Cu (I)/1,10-phenanthroline as an efficient catalytic system, leading to an array of β-hydroxysulfones in moderate to high yields. The significant advantages of this protocol are the mild reaction conditions, readily available starting materials, good functional-group compatibility, synthetic convenience, and practicability.

Assessing the clinical support capabilities of ChatGPT 4o and ChatGPT 4o mini in managing lumbar disc herniation.

Purpose: This study evaluated and compared the clinical support capabilities of ChatGPT 4o and ChatGPT 4o mini in diagnosing and treating lumbar disc herniation (LDH) with radiculopathy.

Methods: Twenty-one questions (across 5 categories) from NASS Clinical Guidelines were input into ChatGPT 4o and ChatGPT 4o mini. Five orthopedic surgeons assessed their responses using a 5-point Likert scale for accuracy and completeness, and a 7-point scale for reliability.

Differentially spliced mitochondrial CYP419A1 contributes to ethiprole resistance in Nilaparvata lugens.

The brown planthopper Nilaparvata lugens is one of the most economically important pests of cultivated rice in Southeast Asia. Extensive use of insecticide treatments, such as imidacloprid, fipronil and ethiprole, has resulted in the emergence of multiple resistant strains of N. lugens.

Removal of Di (2-ethylhexyl) phthalate from groundwater by sodium persulfate activated by hollow micron zero-valent iron: Reaction mechanism and degradation path.

In this study, hollow micron zero-valent iron (H-mZVI) was prepared using the ethylenediamine liquid phase reduction method. The microstructures were characterized by SEM, XRD, BET and FTIR. The results showed that H-mZVI possessed a spherical hollow structure with a particle size of approximately 1 μm.

Respiratory symptoms after coalmine fire and pandemic: A longitudinal analysis of the Hazelwood Health Study adult cohort.

The aim of this study was to determine whether the effects of extreme but discrete PM2.5 exposure from a coal mine fire on respiratory symptoms abated, persisted, or worsened over time, and whether they were exacerbated by COVID-19. We analysed longitudinal survey data from a cohort residing near a 2014 coalmine fire in regional Australia.

Microenvironment Mechanical Torque from ZnFeO (ZFO) Micromotors Inhibiting Tumor Migration.

Mechanical force attracts booming attention with the potential to tune the tumor cell behavior, especially in cell migration. However, the current approach for introducing mechanical input is difficult to apply in vivo. How the mechanical force affects cell behavior in situ also remains unclear.

Radially Distributed Electron Transfer on Single-Crystalline Surface of Gold Microplates.

Electron transfer is ubiquitous in many chemical reactions and biological phenomena; however, the spatial heterogeneities of electron transfer kinetics in electrocatalysis are so far insufficiently resolved. Measuring and understanding the localized electron transfer are crucial to deciphering the intrinsic activity of electrocatalysts and to achieving further improvements in performance. By using scanning electrochemical probe microscopy to spatially resolve redox electrochemistry across the single-crystalline surface of gold microplates, we discover an intriguing radially distributed electron transfer pattern, where the kinetics around the periphery region are significantly higher than those at the central region, regardless of the redox reaction types.

The efficacy of Tripterygium Glycosides in the treatment of Chinese patients with thyroid-associated orbitopathy: a systematic review and meta-analysis.

Objective: This study aims to conduct a systematic review of the effectiveness and safety of Tripterygium Glycosides interventions in the treatment of Chinese patients with thyroid-associated orbitopathy (TAO).

Methods: A literature search was conducted using PubMed for English sources, and the CNKI, Chinese Biomedical Database, Wanfang Database, and VIP Database for Chinese sources. The search period extended from the beginning of the databases' creation to Dec.

Self-Management Behaviours in Rheumatoid Arthritis Patients: What Role Do Health Beliefs Play?

Purpose: Self-management is the intrinsically controlled ability of an active, responsible, informed and autonomous individual to live with the medical, role and emotional consequences of his chronic conditions in partnership with his social network and the healthcare providers. This study evaluated the self-management behaviours of patients with rheumatoid arthritis and assess the association between health beliefs and self-management behaviours.

Methods: This cross-sectional study investigated 269 rheumatoid arthritis patients' self-management behaviours using the Self-Care Behaviours Scale with a score of 0-4 for each item and a total score of 0-100 points, and health beliefs using the Arthritis Health Belief Inventory with a score of 1-5 for each item and a total score of 0-165 points.

Sacubitril/valsartan on right ventricular-pulmonary artery coupling and albumin-bilirubin score in heart failure in Chinese patients with reduced ejection fraction.

Objective: Impaired right ventricular (RV)-pulmonary arterial (PA) coupling, calculated by measuring the tricuspid annular plane systolic excursion (TAPSE) to pulmonary artery systolic pressure (PASP), can be used as an early indicator of right ventricular dysfunction (RVD) in patients with heart failure with a reduced ejection fraction (HFrEF). Patients suffering from HFrEF experience improvements in left ventricular (LV) function through the administration of sacubitril/valsartan therapy. In addition, the albumin-bilirubin (ALBI) score was associated with the fluid overload status and adverse clinical outcomes in patients with heart failure.

Prediction of endohedral borafullerenes X@BC (X = CH, BH, HO, and NH) with a BC shell isovalent with C.

Context: Inspired by the newly synthesized endohedral fullerene T CH@C (1) and based on extensive density functional theory calculations, we predict herein a series of endohedral borafullerenes C CH@BC (4), T BH@BC (5), C HO@BC (6), C NH@BC (7), and T C@BC (8) which possess a BC (3) shell isovalent with C, with the neutral D C@BC (9) obtained from C@BC (8) by symmetric C─B substitutions. Detailed adaptive natural density partitioning (AdNDP) bonding analyses and iso-chemical shielding surfaces (ICSSs) calculations indicate that these core-shell species are spherically aromatic in nature, rendering high stability to the systems. More interestingly, based on the calculated effective donor-acceptor interaction between LP(O) → LV(B@BC) in HO@BC (6), we propose the concept of boron bond (BB) in chemistry which is defined as the in-phase orbital overlap between an electronegative atom A as lone-pair (LP) donor and an electron-deficient boron atom with a lone vacant (LV) orbital as LP acceptor.

Self-limiting selective phase separation of graphene oxide and polymer composite solution.

Homogeneous mixtures undergo phase separation to generate rich heterogeneous structures as well as enable complex physiological activity and delicate design of artificial materials. Beyond free space, the strong coupling between migrating components and spatial confinement plays a crucial role in determining the essential spatial compartment of phase separation, warranting further continuous exploration. Herein, we report the selective phase separation (SPS) behavior of polymers under a mobile two-dimensional (2D) confinement by graphene oxide (GO) sheets.

Preparation of protein and its preventive effect on nonalcoholic fatty liver disease in mice.

is a valuable edible fungus with multidimensional bioactivities; however, research on protein and its beneficial effects on nonalcoholic fatty liver disease (NAFLD) have been limited. In this study, protein (MEP) with 80.59% protein content was prepared, isolated, and characterized by the complete amino acid composition.

Unsupervised Ensemble Learning for Efficient Integration of Pre-trained Polygenic Risk Scores.

The growing availability of pre-trained polygenic risk score (PRS) models has enabled their integration into real-world applications, reducing the need for extensive data labeling, training, and calibration. However, selecting the most suitable PRS model for a specific target population remains challenging, due to issues such as limited transferability, data het-erogeneity, and the scarcity of observed phenotype in real-world settings. Ensemble learning offers a promising avenue to enhance the predictive accuracy of genetic risk assessments, but most existing methods often rely on observed phenotype data or additional genome-wide association studies (GWAS) from the target population to optimize ensemble weights, limiting their utility in real-time implementation.