Ripening-stage variations in small metabolites across six banana cultivars: A metabolomic perspective.

Currently, understanding of how banana cultivars differ in metabolism during ripening is limited. This study compared the pulp metabolites of six banana cultivars using NMR. Bananas with B genome were found to have higher amounts of total starch, amylose, amylopectin, and resistant starch compared to those without B genome.

Regulation of starch metabolism in banana fruit: Mechanisms shaping the nutritional quality.

Bananas are nutrient-rich fruits that provide starch, essential vitamins, and minerals and play significant importance in the global economy through extensive production, trade, and consumption. Nutrient metabolic processes, such as starch-to-sugar conversion, are fundamental in shaping the quality of banana fruits. Starch accounts for 15%-35% of fresh fruit weight, and its degradation mediated by ethylene signaling components can increase sweetness, soften texture, and increase the palatability of banana fruit.

MaGA20ox2f, an OsSD1 homolog, regulates flowering time and fruit yield in banana.

Unlabelled: Previous studies illustrated that two banana GA20 oxidase2 (MaGA20ox2) genes, and , are implicated in controlling banana growth and development; however, the biological function of each gene remains unknown. Ma04g15900 protein (termed MaGA20ox2f in this article) is the closest homolog to the Rice SD1 (encoded by 'green revolution gene', ) in the banana genome. The expression of is confined to leaves, peduncles, fruit peels, and pulp.

Isolation, Characterization, and Proteomic Analysis of Crude and Purified Extracellular Vesicles Extracted from f. sp. .

Extracellular vesicles (EVs) produced by f. sp. () play vital roles in plant-pathogen interactions; however, the isolation of purified TR4-EVs and their pathogenicity and proteomic profiles are not well studied.

Genome-wide analysis of the trihelix gene family reveals that MaGT21 modulates fruit ripening by regulating the expression of MaACO1 in Musa acuminata.

The trihelix transcription factor (GT) gene family members play vital roles in plant growth and development, responses to abiotic or biotic stress, and fruit ripening. However, its role in banana fruit ripening remains unclear. Here, 59 MaGT gene members were identified in banana and clustered into five subfamilies, namely GT1, GT2, GTγ, SIP1, and SH4.

Virulence of banana wilt-causing fungal pathogen Fusarium oxysporum tropical race 4 is mediated by nitric oxide biosynthesis and accessory genes.

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most damaging plant diseases known.

Identification and characterization of two P450 enzymes from involved in TMTT and DMNT biosyntheses and Asian citrus psyllid defense.

The homoterpenes (3)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (,)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are the major herbivore-induced plant volatiles that help in defense directly by acting as repellants and indirectly by recruiting insects' natural enemies. In this study, DMNT and TMTT were confirmed to be emitted from citrus () leaves infested with Asian citrus psyllid ( Kuwayama; ACP), and two cytochrome P450 (CYP) genes ( and ) were newly identified and characterized. Understanding the functions of these genes in citrus defense will help plan strategies to manage huanglongbing caused by Liberibacter asiaticus (Las) and spread by ACP.

MaSMG7-Mediated Degradation of MaERF12 Facilitates f. sp. Tropical Race 4 Infection in .

Modern plant breeding relies heavily on the deployment of susceptibility and resistance genes to defend crops against diseases. The expression of these genes is usually regulated by transcription factors including members of the AP2/ERF family. While these factors are a vital component of the plant immune response, little is known of their specific roles in defense against f.

The Autophagy-Related Protein MaATG8F Interacts with MaATG4B, Regulating Banana Disease Resistance to f. sp. Tropical Race 4.

Banana is one of the most important fruits in the world due to its status as a major food source for more than 400 million people. f. sp.

Two haplotype-resolved genome assemblies for AAB allotriploid bananas provide insights into banana subgenome asymmetric evolution and Fusarium wilt control.

Bananas (Musa spp.) are one of the world's most important fruit crops and play a vital role in food security for many developing countries. Most banana cultivars are triploids derived from inter- and intraspecific hybridizations between the wild diploid ancestor species Musa acuminate (AA) and M.

Efficient and transgene-free genome editing in banana using a REG-2 promoter-driven gene-deletion system.

Unlabelled: [Image: see text]

Supplementary Information: The online version contains supplementary material available at 10.1186/s43897-023-00065-0.

High-quality genome assemblies for two Australimusa bananas (Musa spp.) and insights into regulatory mechanisms of superior fiber properties.

Bananas (Musa spp.) are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries. The fruits are a popular food, and the plants themselves have diverse uses.

Accessory genes in tropical race 4 contributed to the recent resurgence of the devastating disease of Fusarium wilt of banana.

Fusarium wilt of banana, caused by f. sp. (), is one of the most damaging plant diseases recorded.

CsTPS21 encodes a jasmonate-responsive monoterpene synthase producing β-ocimene in citrus against Asian citrus psyllid.

Huanglongbing (HLB), spread by the Asian citrus psyllid (ACP), is a widespread, devastating disease that causes significant losses in citrus production. Therefore, controlling the ACP infestation and HLB infection is very important for citrus production. The aim of our study was to identify any citrus volatile which could be used as a repellent or less attractant towards ACP, and to envisage the potential of this strategy to control HLB spread.

Genome-wide analysis of the polyphenol oxidase gene family reveals that MaPPO1 and MaPPO6 are the main contributors to fruit browning in .

Introduction: Polyphenol oxidases (PPOs), which are widely present in plants, play an important role in the growth, development, and stress responses. They can catalyze the oxidization of polyphenols and result in the browning of damaged or cut fruit, which seriously affects fruit quality and compromises the sale of fruit. In banana (, AAA group), 10 genes were determined based on the availability of a high-quality genome sequence, but the role of genes in fruit browning remains unclear.

Functional Characterization of the Ryanodine Receptor Gene in .

The Asian citrus psyllid (Hemiptera: Liviidae) is a major citrus pest spread around the world. It is also a vector of the bacterium ' Liberibacter asiaticus', considered the cause of the fatal citrus disease huanglongbing (HLB). Insect ryanodine receptors (RyRs) are the primary target sites of diamide insecticides.

Evaluation of Resistance of Banana Genotypes with AAB Genome to Fusarium Wilt Tropical Race 4 in China.

Banana cultivars with the AAB genome group comprise diverse subgroups, such as Plantain, Silk, Iholena, and Pisang Raja, among others, which play an important role in food security in many developing countries. Some of these cultivars are susceptible to f. sp.

WRKY transcription factor MaWRKY49 positively regulates pectate lyase genes during fruit ripening of Musa acuminata.

Fruit ripening is the last phase of fruit growth and development. The initiation and progression of fruit ripening are highly modulated by a plethora of key genes, such as transcription factor (TF) genes. The WRKY gene family is a large group of TFs that play important roles in various cellular processes; nevertheless, the role of WRKY TF on fruit ripening remains enigmatic.

Metabolic profiling reveals genotype-associated alterations in carotenoid content during banana postharvest ripening.

Banana fruits have attracted considerable attention for health-promoting effects attributed to ubiquitous functional metabolites. However, genotype-dependent accumulation patterns of carotenoids in banana remain largely unclear. Here, we performed a systematic metabolomic investigation of 18 banana cultivars of the AAA, AAB, or ABB genome groups.

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence.

Banana is one of most popular fruits globally due to health-promoting and disease-preventing effects, yet little is known about metabolic changes across banana varieties. Here, we integrated gold nanoparticle (AuNP)-assisted laser desorption/ionization mass spectrometry imaging (LDI-MSI) and metabolomics to investigate the spatiotemporal distribution and levels of metabolites within Brazil and Dongguan banana pulps during postharvest senescence. Metabolomics results indicated that both postripening stages and banana varieties contribute to metabolite levels.

Application of developmental regulators to improve in planta or in vitro transformation in plants.

Plant genetic transformation is a crucial step for applying biotechnology such as genome editing to basic and applied plant science research. Its success primarily relies on the efficiency of gene delivery into plant cells and the ability to regenerate transgenic plants. In this study, we have examined the effect of several developmental regulators (DRs), including PLETHORA (PLT5), WOUND INDUCED DEDIFFERENTIATION 1 (WIND1), ENHANCED SHOOT REGENERATION (ESR1), WUSHEL (WUS) and a fusion of WUS and BABY-BOOM (WUS-P2A-BBM), on in planta transformation through injection of Agrobacterium tumefaciens in snapdragons (Antirrhinum majus).

First Report of Fusarium Wilt of Iholena Banana (Musa spp.) Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 in China.

Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), has been considered as the most devastating disease affecting bananas (Musa spp.

FocECM33, a GPI-anchored protein, regulates vegetative growth and virulence in Fusarium oxysporum f. sp. cubense tropical race 4.

ECM33, a glycosylphosphatidylinositol (GPI)-anchored protein, is important for fungal development and infection through regulating fungal cell wall integrity, however, the functions of its orthologs in pathogenesis have not been characterized in Fusarium oxysporum. Here, we discovered a GPI-anchored protein, FocECM33, which is required for vegetative growth and virulence of Fusasium oxysporum f. sp.