Combined Drug with Antibacterial Effect Supports the Normal Intestinal Microflora.

Widespread use of antibiotics leads to an imbalance of normal intestinal microflora and to the development of multidrug resistance. The problem can be solved by administration of the antibiotics in combination with the drugs that have an immunotropic effect. We studied the effect of the drug containing technologically processed affinity purified antibodies to IFNγ, CD4 receptor, β2-microglobulin of MHC class I, and β2-domain of MHC II combined with antibiotics on the composition of intestinal microflora of pigs and the total number of microbiome resistance genes.

[Susceptibility of animal species to experimental SARS-CoV-2 () infection].

Due to the new coronavirus infection pandemic, the global scientific community has been forced to change the direction of the most research, focusing on vaccine development as well as the search for new antiviral drugs to treat COVID-19. The choice of experimental models, timeframe and approaches for evaluating drugs and vaccines under development is crucial for the development of effective measures to prevent and control this disease.The purpose of this review was to summarize the relevant data concerning the susceptibility of laboratory animals to SARS-CoV-2.

IVIG ameliorate inflammation in collagen-induced arthritis: projection for IVIG therapy in rheumatoid arthritis.

Rheumatoid arthritis (RA) is a systemic inflammatory autoimmune disease that leads to joint destruction and disability. Despite a significant progress in administration of biological agents for RA patients, there is still a need for improved therapy. Intravenous immunoglobulins (IVIG), a pooled polyspecific immunoglobulin (Ig)G extracted from 5000 to 20 000 healthy subjects, showed beneficial therapeutic effect in patients with immune deficiency, sepsis and autoimmune diseases.

[Release-active antibodies to S100 protein are able to improve the experimental allergic encephalomyelitis].

Aim: To reveal the effects of release-active antibodies to S100 protein in an animal model of multiple sclerosis.

Material And Methods: Sixty female Wistar rats, aged 12 weeks, were included in the study. The pathology was induced by subcutaneous injection of the spinal cord homogenate.

Analysis of malignancy-associated DNA changes in the nuclei of buccal epithelium in the pathology of the thyroid and mammary glands.

The object of the investigation reported in this paper was to study, from the point of view of statistical and geometric theory of pattern recognition, the DNA optical density distribution peculiarities in the interphase nuclei of buccal epithelium present in the pathology of the thyroid and mammary glands. Two new indices to characterize this distribution (ratio of modal class volumes and relief index) are proposed. It is shown that in malignant neoplasms of the thyroid and mammary glands the changes in the nuclei of buccal epithelium are characterized by an increase in the optical density of DNA over a range from 0.

[Influence of tumor drug resistance phenotype on the dynamics of cisplatin-induced changes of rat peripheral lymphocyte chromatin structure in Guerin's carcinoma].

The correlation between the tumor drug sensitivity and the degree of lymphocyte interphase nuclei chromatin damages induced by cisplatin in rats was found using sensitive and resistant to cisplatin variants of Guerin's carcinoma. Increased optical density of lymphocyte chromatin in first minutes after cisplatin injection both in rats without Guerin's carcinoma and with sensitive to cisplatin variants of this tumor was observed. Lymphocyte chromatin structure remains unchanged in rats with cisplatin resistant carcinoma.

[Tumor-associated changes in the buccal epithelium in human breast and thyroid pathology].

The estimation of the malignancy-associated changes in oral mucosa of patients with benign and malignant processes in mammary and thyroid gland is carried out on the basis of textural and densitometric indices of the interphase nuclei of the epitheliocytes with the help of the methods of mathematical statistics.

[Corpus uteri cancer morbidity and a genetic-mathematical analysis of the proband's pedigrees].

Steady increase of the number of women that primary fell ill with uterus cancer has noted in Ukraine in 1980-1994. The clinic-genealogical analysis of the 262 families with uterus cancer patients of the Kiev region was made. Multifactorial type of heredity predominates.

[The morphological characteristics of a papillomavirus infection in the epithelial tissue of the cervix uteri in comparison with the data from the molecular biological identification of specific HPV DNA sequences].

Summarized cytomorphologic, molecular-biological data and the analysis results of cervix uteri multilayer squamous epithelium cells mitotic regime in benign (104 observations) and dysplastic (101 observations) processes, including 6/11 and 16/18 types DNA HPV, are presented in the paper. It has been shown that the use of HPV-infection morphologic markers as compared with cell mitotic activity and with in situ hybridization results allows one to determine the obvious virus role in the induction of flat, acute and papillar condylomas, as well as of cervix uteri dysplastic processes. To ascertain the prognosis of development of dysplasia associated with HPV DNA of 6/11 and 6/18 types, the increase of mitosis pathological forms and of their arrangement zones in epithelium layer parallel with gravity increase of three-group, multipolar and K mitosis should be considered a reliable marker of high oncogenic risk.

[The cytological characteristics of the cells of the multilayer squamous epithelium of the cervix uteri in relation to the association of the pathological processes with the human papilloma virus].

An analysis of cytological material in benign (160 observations) and dysplastic (101 observations) processes, as well as in cervix outset cancer (38 observations) with the aim of search for koilocytic atypia markers in the cells, existing due to HPV infection, has been carried out. High association degree of the virus infection with the material under study has been ascertained. It was found out that the increase of koilocytosis phenotypic markers expression rate is a reliable criterion of cervix uteri pathologic processes progression.

[A cytofluorometric study of the stability of secondary DNA structure in the epithelial cells in endometrial hyperplasia and cancer].

Studies were performed on epithelial cells obtained from uterine cavity in 49 women. All cases were subdivided into four groups; unaltered endometrium-9, glandular endometrial hyperplasia-10, atypical hyperplasia-4 and endometrial adenocarcinoma-30. For fluorimetric evaluation of single- and double-strand DNA specimens were dyed with acridine orange, which is known to produce red fluorescence in combination with double-strand acids.

[The incidence and clinical genealogical analysis of ovarian cancer in the Kharkiv region].

The analysis of oncoepidemiological state of ovarian cancer (OC) in Kharkov region in comparison with Ukraine within the periods before and after the Chernobyl NPP accident was conducted. Territorial differentiation of this pathology in the regions of Ukraine are represented. An increase in OC incidence among the women of Ukraine and Kharkov region was detected, especially increase of OC the south-east regions.

[A comparison of the morphofunctional characteristics of the peripheral blood lymphocytes and the immune status of patients with malignant melanoma of the skin].

As a results of the study of immune status of 75 patients with malignant skin melanoma, some signs of immunodeficiency with failure of cell and humoral reactions were revealed. The degree of quantitative and qualitative changes of immune system depended on clinical-morphological features of malignancy development.

[Segregation and genetic dispersion analysis of the pedigrees of breast cancer patients in the Kiev region].

Complex clinical-genealogical and genetic study of 261 patients with breast cancer (BC) was carried out in Kiev region. It was demonstrated that the multifactorial origin of BC was of first importance in this population. The share of genetic component in the susceptibility to BC comprise 55.

[The functional characteristics of the chromosomal nucleolus organizer regions of patients with endometrial cancer].

The nucleolus organizer region activity and the level of acrocentric chromosome association in cultured peripheral blood lymphocytes was studied in patients with endometrial glandular hyperplasia and endometrial cancer. The number of active nucleolus organizer regions and the frequency of 13 and 21 acrocentric chromosome associations was higher in patients with endometrial cancer.

[A cytogenetic examination of persons subjected to radiation exposure in certain regions of Ukraine].

We have summarized the results of cytogenetic studies of peripheral blood lymphocytes conducted in the institutions of Kiev and Kharkov in persons irradiated after the accident of the Chernobyl NPP. The average level of chromosomal aberrations and the appearance of cytogenetic markers characteristic of their irradiation effect was higher in the examinees in comparison with the control. The increase in the number of aberrations in certain Kiev residents as well as the development of lymphogranulomatosis in some liquidators of the accident were detected.

[The effect of a distant tumor on the DNA content in the epitheliocytes of the oral mucosa].

Patients with malignant melanomas and breast cancer (83 men and women) were examined. On the basis of study of the DNA contents in nuclei of epitheliocytes from the mucous oral cavity, index of their nuclei heterogeneity we have revealed disturbances in maturation and differentiation of cells. They were arranged to the spinous layer of the oral mucosa.

[The role of the genetic component in oncology].

The paper presents information on fundamentals of oncogenetics, role of the clinical and genealogical method in oncology, in epidemiological genetics in particular. It is shown necessary to create registers of family cases of tumours proceeding from genetical data: risk groups on appearance of malignization. The ways for the further study of the role of a hereditary factors in tumour growth are outlined.

[Cytofluorimetric research on the epithelium of the cervix uteri in malignancy].

The cervical dysplasia and cancer of the uterine cervix of 82 patients have been studied by the cyto-fluorimetric technique. There were 46 patients with dysplasia of mild, moderate and severe degree and 36 patients with different histopathologic type cancer of the uterine cervix T1-2N0M0. DNA content was determined in cytological smears and scrapings of cervix uterus epithelium using luminescent microscope equipped with photometric device FMEI-1.

[Morphofunctional features of the nucleoli in glandular hyperplasia and endometrial cancer].

The morphofunctional peculiarities of epithelial cells nucleoli in patients with glandular hyperplasia and endometrial cancer has been studied on the cytological smears from endometrium stained with silver nitrate were investigated. The patterns of nucleolonemic nucleoli content and high activity of nucleolar organizers in cancer of corpus uteri are revealed.

[Familial-populational studies of endometrial cancer: segregation and genetic dispersion analyses].

A clinico-genealogic investigation was carried out in 216 patients with endometrial cancer. Familial accumulation of endometrial and other cancer incidence was established. The segregation rates appeared to be lower than those expected from simple Mendelian models (2-11%).

[The determination of beta-casein gene expression and the assessment of hormonal homeostasis in gynecomastia patients and in breast cancer in men].

beta-Casein genes expression in breast epithelium was studied in male patients with various forms of gynecomastia and cancer. Blood serum levels of pituitary, sex and glucocorticoid hormones were assayed in 29 patients with gynecomastia and 22 cases of breast cancer, and in 25 of them beta-casein genes expression was evaluated additionally. Activation of the above genes was established in the tissues studied.

[DNA content in nuclei of buccal epithelial cells in practically healthy people].

The results obtained have permitted establishing that changes of DNA content in epitheliocyte nuclei in practically healthy people can be induced by hormonal background in the organism, on the one hand, and by the level of cell differentiation in this globe of mucous membrane of the oral cavity, on the other hand.