Sensitive electrochemical immunosensor for rapid detection of Salmonella in milk using polydopamine/CoFe-MOFs@Nafion modified gold electrode.

Food contaminated by pathogenic bacteria poses a serious threat to human health. Consequently, we used Salmonella as a model and developed an electrochemical immunosensor based on a polydopamine/CoFe-MOFs@Nafion nanocomposite for the detection of Salmonella in milk. The CoFe-MOFs exhibit good stability, large specific surface area, and high porosity.

NMR Immunosensor Based on a Targeted Gadolinium Nanoprobe for Detecting Salmonella in Milk.

Detecting harmful pathogens in food is not only a crucial aspect of food quality management but also an effective way to ensure public health. In this paper, a complete nuclear magnetic resonance biosensor based on a novel gadolinium (Gd)-targeting molecular probe was developed for the detection of Salmonella in milk. First, streptavidin was conjugated to the activated macromolecular polyaspartic acid (PASP) via an amide reaction to generate SA-PASP.

Rapid detection of Salmonella in milk by labeling-free electrochemical immunosensor based on an FeO-ionic liquid-modified electrode.

Electrochemical sensors show distinct advantages over other types of sensors in the rapid detection of microorganisms. Here, we attempted to construct a label-free electrochemical immunosensor based on an FeO-ionic liquid (IL)-modified electrode to rapidly detect Salmonella in milk. The excellent ionic conductivity of the IL facilitated sensor construction, and the large surface area of nano-FeO provided numerous sites for subsequent experiments.

Study on the effect of different concentrations of choline glycine ionic liquid-water mixtures on debranched starch butyrylation reaction.

In this study, the effect of choline glycine ionic liquids on the butyrylation of starch was investigated by the butyrylation of debranched cornstarch in different concentrations of choline glycine ionic liquid-water mixtures (choline glycine ionic liquids to water in mass ratios of 0:10, 4:6, 5:5, 6:4, 7:3, 8:2 and 10:0). The butyryl characteristic peaks in H NMR and FTIR of the butyrylated samples indicated the success of butyrylation modification. H NMR calculations showed that the most effective mass ratio of choline glycine ionic liquids to water (6:4) increased the butyryl substitution degree from 0.

A fluorescent biosensor based on quantum dot-labeled streptavidin and poly-l-lysine for the rapid detection of Salmonella in milk.

Salmonella, as a common foodborne pathogen in dairy products, poses a great threat to human health. We studied a new detection method based on quantum dots (QD). A fluorescent biosensor with multiple fluorescent signal amplification based on a streptavidin (SA) biotin system and the polyamino linear polymer poly-l-lysine (PLL) were established to detect Salmonella in milk.

A novel electrochemical immunosensor based on FeO@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk.

Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a FeO@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability of graphene and the large specific surface area of FeO, the FeO@graphene nanocomposite exhibits an excellent electrical signal, which greatly increased the sensitivity of the immunosensor.

The mechanism of delaying starch digestion by luteolin.

In this study, the mechanisms of the delay of starch digestion by luteolin were revealed by studying the luteolin-PPA (porcine pancreatic α-amylase) interaction and luteolin-starch interaction. The luteolin-PPA interaction was investigated by inhibitory kinetics analysis, fluorescence quenching, circular dichroism (CD), Fourier transform infrared (FT-IR) spectroscopy and molecular docking. The results of the inhibitory kinetics revealed that luteolin was a mixed-type inhibitor of PPA and that the inhibitory action was reversible.

Rapid detection of Salmonella in milk by a nuclear magnetic resonance biosensor based on the streptavidin-biotin system and O-carboxymethyl chitosan target gadolinium probe.

Rapid and sensitive detection of foodborne pathogens is of great importance for food safety. Here, a set of nuclear magnetic resonance (NMR) biosensors based on a O-carboxymethyl chitosan target gadolinium (Gd) probe was developed to quickly detect Salmonella in milk by combining NMR technology and bioimmunotechnology with membrane filtration technology. First, O-carboxymethyl chitosan (O-CMC) was biotinylated to prepare biotinylated O-carboxymethyl chitosan (biotin-O-CMC) through amide reaction, and biotinylated magnetic complexes (biotin-O-CMC-Gd) were obtained by using O-CMC, which has strong chelating adsorption on Gd.

Rapid detection of Salmonella in milk with a nuclear magnetic resonance biosensor based on a streptavidin-biotin system and a polyamidoamine-dendrimer-targeted gadolinium probe.

Rapid and sensitive detection technology is the key to preventing food-borne disease outbreaks. In this study, a low-field nuclear magnetic resonance (NMR) biosensor based on polyamidoamine dendrimers was prepared for the rapid detection of Salmonella in milk. The polyamidoamine dendrimer was biotinylated by amide reaction and chelated to diethylene triamine pentacetate acid and gadolinium to form magnetic complexes.

Effects of xanthan and konjac gums on pasting, rheology, microstructure, crystallinity and in vitro digestibility of mung bean resistant starch.

The effects of different concentrations of xanthan and konjac gums on the pasting, rheological properties, microstructure, crystallinity, and digestibility of mung bean resistant starch (MRS) were investigated. Based on the results of pasting properties, the adjunction of gums increased the peak, breakdown, and final viscosities of resistant starch. Compared with resistant starch, the addition of gum significantly increased the K value and dynamic moduli (G', G") of MRS with increasing gum concentration.

Determination of chlorine ions in raw milk by pulsed amperometric detection in a flow injection system.

Chloride ion concentration in milk was determined by pulsed amperometric detection in a flow injection system. Results showed that the Au electrode lost 3 electrons at 1.10 V and formed chloroaurate ions (AuCl) by combining with chloride ions, after which AuCl was partly reduced to Au at 0.