Effectiveness of Three-dimensional Cardiac Computed Tomography Scan in Congenital Heart Surgery-An Impact on Diagnostic Performance and Surgical Management.

Background: Recent advances in gated cardiac contrast-enhanced computed tomography (CECT) with anesthesia support, enhance the imaging performance in congenital heart disease (CHD). 3D reconstruction of the CECT image is a novel modality that could help manage pediatric cardiac patients.

Methods: A retrospective study of children diagnosed with CHD presenting for surgical intervention (n = 139) was carried out at our cardiac surgical center.

Na detection brightening of synergistically originated noble metal nanoclusters.

Weakly fluorescent AuAg nanoclusters were obtained from glutathione, chloroauric acid, and silver nitrate aqueous solution under a modified hydrothermal method. Such glutathione-capped synergistically evolved clusters were obtained for the first time by employing our experimental conditions. Such weak fluorescence was made significantly brighter by employing Na and a Na sensor was obtained with a linear detection range of 10-5 × 10 M, while the limit of detection was 1.

Novel environmental applications of green tea: sensing and remediation of Ag in aqueous system.

The strong fluorescence of green tea was quenched with Fe because of ligand-to-metal charge transfer and subsequent formation of magnetite (FeO) nanoparticles (heavy metal effect). Ag restored the lost fluorescence by confining iron particles (capped with Cl) with the formation of AgCl. Thus, toxic Ag was sensed in the aqueous system with a linear detection range of 10 M to 10 M and a detection limit of 4.

Synergism between copper and silver nanoclusters induces fascinating structural modifications, properties, and applications.

Among the group 11 transition metal elements, Cu and Ag are widely studied due to their cost effectiveness and easy availability. However, the synergism between copper and silver is also very promising, exhibiting intriguing structures, properties, and applications. Nanoclusters, which are missing links between atoms and nanoparticles, are highly fluorescent due to their discrete energy levels.

Recent applications of coinage metal nanoparticles passivated with salicylaldehyde and salicylaldehyde-based Schiff bases.

Salicylaldehyde (SD) and its derivatives are effective precursors for generating coinage metal (gold, silver, and copper) nanoparticles (NPs). These NPs have a variety of potential environmental applications, such as in water purification and sensing, and those arising from their antibacterial activity. The use of SD and its derivatives for synthesizing coinage NPs is attractive due to several factors.

Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor.

The Duffy antigen receptor is a seven-transmembrane (7TM) protein expressed primarily at the surface of red blood cells and displays strikingly promiscuous binding to multiple inflammatory and homeostatic chemokines. It serves as the basis of the Duffy blood group system in humans and also acts as the primary attachment site for malarial parasite Plasmodium vivax and pore-forming toxins secreted by Staphylococcus aureus. Here, we comprehensively profile transducer coupling of this receptor, discover potential non-canonical signaling pathways, and determine the cryoelectron microscopy (cryo-EM) structure in complex with the chemokine CCL7.

Role of silver nanoparticles and silver nanoclusters for the detection and removal of Hg(ii).

Silver metal, being a 3d transition metal in group 11 in the periodic table, is widely used in material science for its distinguished plasmonic properties. Nanoparticles (NPs) and nanoclusters (NCs) are widely used in sensing applications having a surface plasmon band and emissive properties, respectively. Mercury is one of the detrimental toxins and threats to various ecosystems.

Analytical developments in the synergism of copper particles and cysteine: a review.

Cysteine, a sulfur-containing amino acid, is a vital candidate for physiology. Coinage metal particles (both clusters and nanoparticles) are highly interesting for their spectacular plasmonic properties. In this case, copper is the most important candidate for its cost-effectiveness and abundance.

Immunophenotypic and Cytogenetic Characteristics of Pediatric Acute Lymphoblastic Leukemia: A Burden Estimation Study from Eastern India.

Background: Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Immunophenotype (IPT) and cytogenetics are essential for diagnosis, risk stratification, and management for ALL.

Objectives: Evaluating the burden of immunophenotypic and cytogenetic profile of pediatric ALL patients.

Photocatalytic degradation of methyl blue dye with HO sensing.

A condensation polymer (urea-formaldehyde resin) passivated ZnO nanoparticles were used as an efficient photocatalyst for methyl blue degradation in the presence of HO involving a Fenton-like reaction. The formation of OH˙ radicals were attributed to the pivotal factor for the degradation process. The method was easy and recyclable.

Role of transition metals in coinage metal nanoclusters for the remediation of toxic dyes in aqueous systems.

A difficult issue in chemistry and materials science is to create metal compounds with well-defined components. Metal nanoclusters, particularly those of coinage groups (Cu, Ag, and Au), have received considerable research interest in recent years owing to the availability of atomic-level precision joint experimental and theoretical methods, thus revealing the mechanisms in diverse nano-catalysts and functional materials. The textile sector significantly contributes to wastewater containing pollutants such as dyes and chemical substances.

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution.

The Hydroxycarboxylic acid receptor 2 (HCA2), also known as the niacin receptor or GPR109A, is a prototypical GPCR that plays a central role in the inhibition of lipolytic and atherogenic activities. Its activation also results in vasodilation that is linked to the side-effect of flushing associated with dyslipidemia drugs such as niacin. GPR109A continues to be a target for developing potential therapeutics in dyslipidemia with minimized flushing response.

Highly fluorescent quinone-capped silver hydrosol for environmental remediation and sensing applications.

A metal-enhanced fluorescence was achieved from in situ-generated Ag nanoparticles in the proximity of 2-hydroxy benzaldehyde (2HB). Such nanoparticles eliminated methyl blue (MB) dye from water exclusively in the presence of Zn and were proven to be an efficient adsorbent for environmental remediation (maximum uptake capacity 1065 mg·g). Ag was zero valent in the absorbent, while Zn was in Zn(OH) form.

Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues.

Mouse is the mammalian model of choice to study human health and disease due to its size, ease of breeding and the natural occurrence of conditions mimicking human pathology. Here we design and validate multiple reaction monitoring mass spectrometry (MRM-MS) assays for quantitation of 2118 unique proteins in 20 murine tissues and organs. We provide open access to technical aspects of these assays to enable their implementation in other laboratories, and demonstrate their suitability for proteomic profiling in mice by measuring normal protein abundances in tissues from three mouse strains: C57BL/6NCrl, NOD/SCID, and BALB/cAnNCrl.

De novo drug designing coupled with brute force screening and structure guided lead optimization gives highly specific inhibitor of METTL3: a potential cure for Acute Myeloid Leukaemia.

Expression of METTL3, a SAM dependent methyltransferase, which deposits m6A on mRNA is linked to poor prognosis in Acute Myeloid Leukaemia and other type of cancers. Down regulation of this epitranscriptomic regulator has been found to inhibit cancer progression. Silencing the methyltransferase activity of METTL3 is a lucrative strategy to design anticancer drugs.

Molecular basis of anaphylatoxin binding, activation, and signaling bias at complement receptors.

The complement system is a critical part of our innate immune response, and the terminal products of this cascade, anaphylatoxins C3a and C5a, exert their physiological and pathophysiological responses primarily via two GPCRs, C3aR and C5aR1. However, the molecular mechanism of ligand recognition, activation, and signaling bias of these receptors remains mostly elusive. Here, we present nine cryo-EM structures of C3aR and C5aR1 activated by their natural and synthetic agonists, which reveal distinct binding pocket topologies of complement anaphylatoxins and provide key insights into receptor activation and transducer coupling.

Multisystem Inflammatory Syndrome in Children (MIS-C): Comparison of the First and the Second Waves.

This study comparing the different parameters of children suffering from multisystem inflammatory syndrome in children (MIS-C) in Kolkata, India, during the two waves (July, 2020-January, 2021 and April-July, 2021) showed that the second wave had a higher propensity of Kawasaki disease (KD)-like presentation, cardiac affection and pediatric intensive care unit admission, and increased incidence of use of steroids for treatment.

Neurovirulence, viscerotropism and immunogenicity of live attenuated yellow fever 17D vaccine virus in non-human primates.

Yellow fever vaccine associated neurovirulence and viscerotropism have been reported by various countries. In this study, the neurovirulence, viscerotropism and immunogenicity of yellow fever vaccine seed lots (master and working) and final product manufactured at Serum Institute of India (SII) were evaluated in cynomolgus monkeys. WHO reference virus 168-73 and Stamaril™ as a control vaccine was used for comparison.

Cholinergic control of Th17 cell pathogenicity in experimental autoimmune encephalomyelitis.

Experimental autoimmune encephalomyelitis (EAE) is a mouse model of multiple sclerosis (MS) in which Th17 cells have a crucial but unclear function. Here we show that choline acetyltransferase (ChAT), which synthesizes acetylcholine (ACh), is a critical driver of pathogenicity in EAE. Mice with ChAT-deficient Th17 cells resist disease progression and show reduced brain-infiltrating immune cells.

Case report of recurrent bleeding in an infant with isolated prolonged prothrombin time due to congenital factor VII deficiency---a .

Factor VII deficiency is a rare bleeding disorder showing an autosomal recessive pattern of inheritance. Data from our country on this particular entity is lacking. Especially the specific mutations associated with this disease are not well documented.

Alpha glucosidase inhibitory properties of a few bioactive compounds isolated from black rice bran: combined and evidence supporting the antidiabetic effect of black rice.

In view of the recent reports of the antidiabetic effect of the black rice bran extract, an attempt has been made in the present work to evaluate the potential α-glucosidase inhibitory activity of a few selected bioactive compounds present in the pericarp of the black rice. Out of the six bioactive compounds from black rice bran selected for the study, two compounds cyanidin-3-glucoside and 6'--feruloylsucrose were identified as novel and highly potent α-glucosidase inhibitors their and screenings. The enzyme inhibition assay was corroborated by molecular docking and molecular dynamics simulation studies.

Complementary description of three species of Steneotarsonemus (Acari: Tarsonemidae) from rice agroecosystems of Eastern India with notes on their taxonomic status, spatial distribution, intraspecific variation and species composition.

Rice agroecosystems are inhabited by a number of Steneotarsonemus species worldwide with various host plant relationships and taxonomic identities. To clarify this situation, a large-scale survey was initiated in rice growing belts of eastern India and this revealed the presence of four species of Steneotarsonemus viz. S.

Dengue Associated Demyelinating Disorders - A Report of 2 Cases.

Dengue is a common viral infection worldwide, though its neurological manifestations are infrequent (2%-11% in recent years) and can be varied as the Dengue virus per se is a non-neurotropic virus. Neurological manifestations of Dengue usually result from multisystem dysfunction which may be secondary to vascular leak or it can be due to direct virus invasion (dengue encephalitis) or an immunological phenomenon which is triggered by dengue infection (demyelinating disorders). Here we present two cases of dengue fever associated demyelinating disorders in two pediatric patients aptly depicting the two spectra of the disease.

Cardiac Evaluation in Multisystem Inflammatory Syndrome in Children (MIS-C) Associated With COVID-19.

Multisystem inflammatory syndrome in children (MIS-C) is notorious for its cardiac involvement. We present a single center data of 71 children, of which 57.7% had myocarditis and 26.