Combatting Helicobacter pylori: A Focus on Nanomaterials.

Developing effective non-antibiotic antimicrobial strategies is essential for combating global antibiotic resistance, including resistance stemming from Helicobacter pylori (H. pylori) treatment. Nanomaterials offer a promising and innovative approach for non-antibiotic anti-H.

Association of nutritional status and comorbidity with long-term survival among community-dwelling older males.

Background: Estimates of survival in the older can be of benefit in various facets, particularly in medical and individual decision-making. We aim to validate the value of a combination of nutrition status evaluation and comorbidity assessment in predicting long-term survival among community-dwelling older.

Methods: The Charlson Comorbidity Index (CCI) was applied for comprehensive evaluation of comorbidities.

Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury.

Polaprezinc (PZ) plays a role in the protection of gastric mucosa and inhibiting Helicobacter pylori (H. pylori) growth in vitro. The objective of this study was to determine the protective effects of PZ on human gastric epithelial cells (GES-1) against H.

Transcriptomic and Functional Approaches Unveil the Role of tmRNA in Zinc Acetate Mediated Levofloxacin Sensitivity in Helicobacter pylori.

Rapid increase in resistance of Helicobacter pylori (H. pylori) has hindered antibiotics-based eradication efforts worldwide and raises the need for additional approaches. Here, we investigate the role of zinc-based compounds in inhibiting H.

Higher Prevalence of Non-thyroidal-Illness Syndrome in Elderly Male Patients With Active Infection.

It is currently unclear whether the infection leads to associated alterations in thyroid functions and thyroidal illnesses. This study aims to analyse this relationship in an elderly male cohort over a five-year period. A case retrospective study.

Effect of Temperature on Metronidazole Resistance in .

Efficacy of eradication therapy has declined due to rapid rises in antibiotic resistance. We investigated how increased temperature affected (NCTC 11637) growth and its sensitivity to metronidazole . We performed transcriptomic profiling using RNA-sequencing to identify differentially expressed genes (DEGs) associated with increased temperature.

Diagnosis of Helicobacter pylori infection in the elderly using an immunochromatographic assay-based stool antigen test.

The diagnostic value of Helicobacter pylori stool antigen (HpSA) tests in elderly subjects remains unclear. The objective of this study was to assess the diagnostic accuracy of the immunochromatographic assay-based HpSA test in a male elderly cohort and identify factors affecting the accuracy. Data for asymptomatic elderly male citizens (≥65 years old) who received health checkups at the Chinese PLA General Hospital between July 2007 and November 2018 were collected.

Lifestyle intervention for gastroesophageal reflux disease: a national multicenter survey of lifestyle factor effects on gastroesophageal reflux disease in China.

Background: Poor habits can worsen gastroesophageal reflux disease (GERD) and reduce treatment efficacy. Few large-scale studies have examined lifestyle influences, particularly eating habits, on GERD in China, and research related to eating quickly, hyperphagia, and eating hot foods is quite limited. The aim of this study was to evaluate the relationship between GERD pathogenesis and lifestyle factors to produce useful information for the development of a clinical reference guide through a national multicenter survey in China.

Expression of GCRG213p, LINE-1 endonuclease variant, significantly different in gastric complete and incomplete intestinal metaplasia.

Background: Intestinal metaplasia (IM) of the gastric mucosa is classified as complete (Type I) and incomplete IM (Type II and III) subtypes, which showed significantly different risk for developing to gastric adenocarcinoma (GAC). GCRG213, a variant of L1-endonuclease (L1-EN), first identified in our lab, was upregulated in GAC tissue. However, the relationship between GCRG213 and IM subtypes is not clear.

Association of active Helicobacter pylori infection and anemia in elderly males.

Background: The prevalence of Helicobacter pylori (H. pylori) infection increases with age. However, the relationship between H.

Maximizing Specific Loss Power for Magnetic Hyperthermia by Hard-Soft Mixed Ferrites.

Maximized specific loss power and intrinsic loss power approaching theoretical limits for alternating-current (AC) magnetic-field heating of nanoparticles are reported. This is achieved by engineering the effective magnetic anisotropy barrier of nanoparticles via alloying of hard and soft ferrites. 22 nm Co Mn Fe O /SiO nanoparticles reach a specific loss power value of 3417 W g at a field of 33 kA m and 380 kHz.

Management and survival analysis of elderly patients with a cancer in the digestive system who refused to receive anticancer treatments.

Treatment and management of cancers in elderly patients require some special considerations. A better understanding of how cancers progress in those elderly patients who have not received any anticancer treatments could better help us in treating these patients and in making end-of-life decisions. Over the past years, we had encountered 57 elderly patients, aged 75 to 94 years (87.

[Deuterium depleted environment inhibits the growth of gastric cancer cells: in vitro study].

Objective: To examine whether deuterium depleted environment may affect the biological features of human gastric cancer cells(SGC-7901)and explore the possible underlying mechanisms.

Methods: SGC-7901 cells were cultured in RPMI-1640 medium prepared with distilled water of different deuterium concentrations(experimental group:25ppm deuterium;control group:150ppm deuterium). Assays on cellular proliferation, cell cycle and apoptosis were conducted at different time points and comparison.

Learning about the Importance of Mutation Prevention from Curable Cancers and Benign Tumors.

Some cancers can be cured by chemotherapy or radiotherapy, presumably because they are derived from those cell types that not only can die easily but also have already been equipped with mobility and adaptability, which would later allow the cancers to metastasize without the acquisition of additional mutations. From a viewpoint of biological dispersal, invasive and metastatic cells may, among other possibilities, have been initial losers in the competition for resources with other cancer cells in the same primary tumor and thus have had to look for new habitats in order to survive. If this is really the case, manipulation of their ecosystems, such as by slightly ameliorating their hardship, may prevent metastasis.

[Effect of hypoxia on gastric cancer cell proliferation and LINE-1 endonuclease variant GCRG213 expression].

Objective: To investigate the change of gastric cancer cell proliferation and the expression of gastric cancer related gene 213 (GCRG213), a long interspersed nuclear element-1 (LINE-1) endonuclease variant, during hypoxia.

Methods: Normal gastric mucosa cell GES-1 and gastric cancer cell BGC-823 were cultured in 20% or 3% oxygen concentrations, respectively. MTT test was used to analyze the proliferation of the GES-1 and BGC-823 cells.

Expression of a LINE-1 endonuclease variant in gastric cancer: its association with clinicopathological parameters.

Background: Long interspersed nuclear element-1 (LINE-1 or L1), the most abundant and only autonomously active family of non-LTR retrotransposons in the human genome, expressed not only in the germ lines but also in somatic tissues. It contributes to genetic instability, aging, and age-related diseases, such as cancer. Our previous study identified in human gastric adenocarcinoma an upregulated transcript GCRG213, which shared 88% homology with human L1 sequence and contained a putative conserved apurinic/apyrimidinic endonucleas1 domain.

Comparison of global gene expression of gastric cardia and noncardia cancers from a high-risk population in china.

Objective: To profile RNA expression in gastric cancer by anatomic subsites as an initial step in identifying molecular subtypes and providing targets for early detection and therapy.

Methods: We performed transcriptome analysis using the Affymetrix GeneChip U133A in gastric cardia adenocarcinomas (n = 62) and gastric noncardia adenocarcinomas (n = 72) and their matched normal tissues from patients in Shanxi Province, and validated selected dysregulated genes with additional RNA studies. Expression of dysregulated genes was also related to survival of cases.

[Preparation and identification of monoclonal antibody against human GCRG213].

Aim: To prepare and characterize the monoclonal antibody against human GCRG213.

Methods: The HIS-GCRG213 fusion protein was expressed in E.coli.

Tissue microarray analysis of topoisomerase IIalpha protein in gastric adenocarcinomas: histogenetic and prognostic implications.

Unlabelled: The aim of this study was to: To investigate topoisomerase IIα (topo-IIα) expression and its correlation with clinicopathological parameters in primary gastric cancer patients.

Patients And Methods: A tissue microarray including tumor, paired non-tumoral and lymph node metastasis specimens from 210 gastric adenocarcinoma patients was built for immunohistochemical interrogation. The correlation between topo-IIα expression and patient clinicopathological parameters was evaluated by univariate and multivariate analyses.

[Preparation, identification and preliminary application of the polyclonal antibody against gastric cancer-related protein GCRG224].

Aim: To prepare the polyclonal antibody against gastric cancer-related protein GCRG224.

Methods: The thioredoxin/GCRG224 fusion protein was expressed in E.coli.

Recurrence in patients following curative resection of early gastric carcinoma.

Objective: Post-gastrectomy recurrences of early gastric cancer occur in a few cases. We investigated the outcome of early gastric cancer patients treated surgically, with special respect to the risk factor(s) for tumor recurrence.

Patients And Methods: A total of 308 patients with mono-foci early gastric cancer underwent curative surgical resection.

[Gene expression profile of human normal gastrointestinal tract tissues: bioinformatic study].

Aim: To identify up-regulated genes specific to human normal gastrointestinal tissues.

Methods: Study was made on human normal tissue gene expression database open to the public. Tissue-specific genes were identified using one-tailed student T test.

LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma.

Aim: To analyze the expression profiles of a human gastric-cancer-related gene, GCRG123, in human gastric signet-ring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.

Methods: In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues.

[Preparation and characterization of rabbit antibody against gastric cancer-related protein GCRG213].

Aim: To prepare the rabbit antibody against gastric cancer-related protein GCRG213.

Methods: The thioredoxin/GCRG213 fusion protein was expressed in E. coli.