Publications by authors named "Gangmin Tang"
[DNA assembly by multi-fragment digestion/ligation and homologous recombination].
Sheng Wu Gong Cheng Xue Bao
May 2024
Article Synopsis
- Developed a new, efficient protocol for multi-fragment assembly and multi-site mutagenesis by optimizing existing methods.
- Utilized fructose-1,6-diphosphatase 1 (FBP1) with four specific mutant sites for validation.
- Successfully addressed the limitations of traditional methods like Gibson and Golden Gate assembly, resulting in a reliable solution for complex genetic modifications.