Publications by authors named "Ganggang Huang"
Article Synopsis
- Demand for monoclonal antibodies (mAbs) is rising in research and medicine, with hybridoma technology being the traditional method since the 1970s.
- Phage display methods have emerged as a viable alternative, especially after the approval of Humira in 2002, which is phage-derived and treats rheumatoid arthritis.
- The protocol outlined describes how to construct synthetic phage-displayed Fab libraries, detailing steps like preparing electro-competent cells, DNA extraction, mutagenesis, electroporation, and evaluating the libraries' diversity using ELISA and DNA sequencing.
