Publications by authors named "Gang-Jian Gu"
Article Synopsis
- - A multi-discipline simulation study was conducted to evaluate three de-airing methods for managing massive air embolism during cardiopulmonary bypass (CPB), aiming to develop a standardized approach for better clinical safety.
- - The study involved ten participants, including licensed perfusionists and trainees, who tested the Conventional Method, A-V Loop Method, and Isolation of the ALF Method, with recovery times significantly different among the methods.
- - Results indicated that the A-V Loop Method was the most efficient, with an average recovery time of 196.8 seconds, while the Conventional Method took over 290 seconds, demonstrating the effectiveness of simulation-based training in optimizing perfusion techniques.
Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve.
J Huazhong Univ Sci Technolog Med Sci
February 2014
Aortic valve calcification is a common disease in the elderly, but its cellular and molecular mechanisms are not clear. In order to verify the hypothesis that Wnt/β-catenin signaling pathway is involved in the process of calcification of aortic valve, porcine aortic valve interstitial cells (VICs) were isolated, cultured and stimulated with oxidized low density lipoprotein (ox-LDL) for 48 h to induce the differentiation of VICs into osteoblast-like cells. The key proteins and genes of Wnt/β-catenin signaling pathway, such as glycogen synthase kinase 3β (GSK-3β) and β-catenin, were detected by using Western blotting and real-time polymerase chain reaction (PCR).
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