Phytoremediation offers a great potential for affordable remediation of heavy metal (HM)-polluted soil and water. Screening and identifying candidate genes related to HM uptake and transport is prerequisite for improvement of phytoremediation by genetic engineering. Using the cadmium (Cd)-hypersensitive Populus euphratica, an annexin encoding gene facilitating Cd enrichment was identified in this study.
View Article and Find Full Text PDFPlasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves.
View Article and Find Full Text PDFSodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin () gene in leaves, roots, and callus cultures of (salt-resistant poplar). To explore the mechanism of the in salinity tolerance, the full length of was cloned from and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells.
View Article and Find Full Text PDFSalt stress is an important environmental cue impeding poplar nitrogen nutrition. Here, we characterized the impact of salinity on proton-driven nitrate fluxes in ectomycorrhizal roots and the importance of a Hartig net for nitrate uptake. We employed two Paxillus involutus strains for root colonization: MAJ, which forms typical ectomycorrhizal structures (mantle and Hartig net), and NAU, colonizing roots with a thin, loose hyphal sheath.
View Article and Find Full Text PDFWe investigated the effects of salt-sensitive signaling molecules on ionic fluxes and gene expression related to K/Na homeostasis in a perennial herb, , during short-term NaCl stress (100 mM, 24 h). Salt treatment caused more pronounced Na accumulation in root cells than in leaf cells. Na ions were mostly compartmentalized in vacuoles.
View Article and Find Full Text PDFUsing a Non-invasive Micro-test Technique, flux profiles of Cd, Ca, and H were investigated in axenically grown cultures of two strains of (MAJ and NAU), ectomycorrhizae formed by these fungi with the woody Cd-hyperaccumulator, × , and non-mycorrhizal (NM) roots. The influx of Cd increased in fungal mycelia, NM and ectomycorrhizal (EM) roots upon a 40-min shock, after short-term (ST, 24 h), or long-term (LT, 7 days) exposure to a hydroponic environment of 50 μM CdCl. Cd treatments (shock, ST, and LT) decreased Ca influx in NM and EM roots but led to an enhanced influx of Ca in axenically grown EM cultures of the two isolates.
View Article and Find Full Text PDFVirus-induced gene silencing (VIGS) has been shown to be an effective tool for investigating gene functions in herbaceous plant species, but has rarely been tested in trees. The establishment of a fast and reliable transformation system is especially important for woody plants, many of which are recalcitrant to transformation. In this study, we established a tobacco rattle virus (TRV)-based VIGS system for two Populus species, Populus euphratica and P.
View Article and Find Full Text PDFPoplar species increase expressions of transcription factors to deal with salt environments. We assessed the salt-induced transcriptional responses of heat-shock transcription factor (HSF) and WRKY1 in Populus euphratica, and their roles in salt tolerance. High NaCl (200mM) induced PeHSF and PeWRKY1 expressions in P.
View Article and Find Full Text PDFPlant Physiol Biochem
October 2013
The plant plasma membrane (PM) H(+)-ATPase plays a crucial role in controlling K(+)/Na(+) homeostasis under salt stress. Our previous microarray analysis indicated that Populus euphratica retained a higher abundance of PM H(+)-ATPase transcript versus a salt-sensitive poplar. To clarify the roles of the PM H(+)-ATPase in salt sensing and adaptation, we isolated the PM H(+)-ATPase gene PeHA1 from P.
View Article and Find Full Text PDFSalt-induced fluxes of H(+), Na(+), K(+), and Ca(2+) were investigated in ectomycorrhizal (EM) associations formed by Paxillus involutus (strains MAJ and NAU) with the salt-sensitive poplar hybrid Populus × canescens. A scanning ion-selective electrode technique was used to measure flux profiles in non-EM roots and axenically grown EM cultures of the two P. involutus isolates to identify whether the major alterations detected in EM roots were promoted by the fungal partner.
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