Antiproliferative potential of 3β,5α,6β,7α-tetrahydroxyergosta-8(14),22-diene produced by isolated from an alkaline crater lake in Puebla, Mexico.

The sterol 3β,5α,6β,7α-tetrahydroxyergosta-8(14),22-diene was obtained from bio-guided fractioning of the chloroform extract of 50 L of liquid culture of . This fungal strain was selected because of its anti-proliferative activity against solid human tumour cell lines (GI ≤ 50 μg/mL) in a bio-prospective study of fungi isolated from plant material, sediment and water samples obtained from alkaline lakes Alchichica and Atexcac in Puebla, Mexico. This compound showed GI (μM) values of: 16, 24, 18, 15 and 12 against tumour cell lines A-549, HBL-100, HeLa, T-47D and WiDr respectively.