Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology.

Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/or genome mutations. The potential of these two methods can be enhanced by the combination with fluorescence in situ hybridization (FISH) techniques.

Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay.

Unlabelled: The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors.

Methods: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used.