Reducing data acquisition for light-sheet microscopy by extrapolation between imaged planes.

Light-sheet fluorescence microscopy (LSFM) is a powerful technique that can provide high-resolution images of biological samples. Therefore, this technique offers significant improvement for three-dimensional (3D) imaging of living cells. However, producing high-resolution 3D images of a single cell or biological tissues, normally requires high acquisition rate of focal planes, which means a large amount of sample sections.