[The development of complex technique of evaluation of virulence of parahemolytic vibrio].

The article deals with results of studying parahemolytic vibrio separatedfrom different sources according their phenotype and genotype attributes associated with virulence. In certain cases the mismatch of results of Kanagava tests and polymerase chain reaction test of gene tdh was established. The need in virulence complex evaluation is substantiated.

[A case of Crimean-Congo hemorrhagic fever in the Anapa District, Krasnodar Territory].

The paper gives information on the first case of Crimean-Congo hemorrhagic fever detected in a female resident of the Anapa District, Krasnodar Territory, in 2005 in the past 57 years.

[Biological and genetic characterization of strains of the pathogen of legionellosis, isolated from water on Russian territory].

As the result of the study of the spread of Legionella in different regions of Russia, 69 cultures were isolated from different water systems. After serotyping most of these strains (85%) were identified as L. pneumophila, serogroups 1 and 6.

[The design of bacteriological preparations for the diagnosis of legionellosis].

The technique of making legionellosis diagnostic preparations is described, making use of traditional methods and new modified procedures for isolation and purification of the components of serological biosystems. Results of using bacteriological preparations in studies of legionellosis are presented.

[Phages of halophilic vibrios].

The morphology and antigenic properties of 34 phages of Vibrio parahaemolyticus and V. alginolyticus strains isolated from Black Sea water, as well as their range of lytic activity and specificity of action, have been studied. Most of these phages have been found to lyze both V.

[The fatty acid composition of the total lipids in Vibrio cholerae].

The fatty acid composition of total cell lipids of V. albensis typing strains, serovars 01-012, isolated from patients and environmental objects, as well as V. eltor and NAG vibrios, has been studied.

[The lipids of phosphorescent Vibrio albensis bacteria].

The lipid composition of fluorescent vibrios, V. eltor and nonagglutinating vibrios has been studied. In the fraction of polar lipids phosphatidylethanolamine, phosphatidylinositol and cardiolipin and in the fraction of neutral lipids monoglycerides, free fatty acids, diglycerides, triglycerides, sterol esters have been identified.

[The isolation of Vibrio fluvialis on the territory of the USSR].

For the first time V. fluvialis strains were detected on the territory of the USSR. The taxonomic position of these vibrios was determined by their nucleotide DNA composition (the content of guanine + cytosine was 49.

Serological diagnosis of diarrhoea caused by some representatives of the genera Vibrio and Aeromonas.

Various serological methods were used to study 630 blood sera of patients with diarrhoea on whom microorganisms of the genera Vibrio (vibrios of non-01 group, phosphorescent, parahaemolytic and alginolytic vibrios) and Aeromonas were isolated, and also of patients with unconfirmed bacteriological diagnosis, carriers and healthy persons. It has been established that specific antibodies are isolated in 30-50% of cases, most frequently in the agglutination reaction, in PHAR with flagellar erythrocyte diagnostic agent, in the reaction of vibriocidal antibodies using the Takachi apparatus. Serological diagnosis is a subsidiary method promoting the performance of clinico-epidemiological analysis of diseases with diarrhoea.

[Taxonomic position of representatives of the species Vibrio cholerae].

The data contained in the works of some foreign authors who have reconsidered the taxonomic position of V. metschnikovii are analyzed. In contrast to the views expressed by Gamaleya whi believed this microorganism to be the modified cholera vibrio, the foreign authors identify it with the "Kommabacillus der Cholera nostras" described by Finkler and Prior (1884) and classify it with saprophytes widely spread in the environment.

[Serological typing of Vibrios, nonagglutinating with O-cholera serum, isolated in USSR].

The authors carried out serological typing of 2008 strains of NAG vibrios isolated in 1968--1975 in 18 territories of the Soviet Union from humans and from the objects of environment. Serological type was established in 40.5% of the srains isolated from humans and in 16% of the strains obtained from the environmental objects.

[Role of neuraminidase from cholera vibrions in the pathogenesis of experimental cholera].

A study was made of a possible effect of neuraminidase of cholera vibrios on cholera pathogenesis. It was shown that in intraintestinal injection of cholera vibrios of the El Tor biotype to nursling rabbits neuraminidase could be revealed in their intestine 5 to 8 hours after the infection. Addition of neuraminidase to the weakly cholerogenic strain cholera vibrios intensified its cholerogenic action in infection of the animals.