Monosomal karyotype in acute myeloid leukemia defines a distinct subgroup within the adverse cytogenetic risk category.

Monosomal karyotype (MK) has recently been reported to identify a distinct subset of acute myeloid leukemia (AML) with adverse prognosis. We retrospectively evaluated the frequency of MK in a large cohort of 549 unselected AML cases diagnosed in our department over a period of 13 years and explored potential associations with clinicobiological features and outcome. MK was found in 62 of 549 cases (11.

Recurrent cytogenetic findings in subsets of patients with chronic lymphocytic leukemia expressing IgG-switched stereotyped immunoglobulins.

We report a remarkable association of recurrent (stereotyped) cytogenetic aberrations with two subsets of chronic lymphocytic leukemia (CLL) cases expressing IgG-switched, stereotyped B-cell receptors (BCRs). Comparison with cases with heterogeneous BCRs showed that these recurrent cytogenetic aberrations were subset-biased. These findings further support a role for antigen in CLL development.

Clinical, immunophenotypic, and molecular profiling of trisomy 12 in chronic lymphocytic leukemia and comparison with other karyotypic subgroups defined by cytogenetic analysis.

In a cohort of 130 unselected chronic lymphocytic leukemia (CLL) patients, 73 cases had normal karyotypes, 57 cases had abnormal karyotypes, and 22/57 cases carried more than one abnormality. Trisomy 12 (+12) was the most common abnormality (26/130 cases; 20%), and 17/26 cases had isolated +12. Del(13q)/t13q/-13 was detected in 19/130 cases (14.

Isolated pentasomy of chromosome 8 in erythroleukemia.

Pentasomy 8 as a sole anomaly in hematological disorders is rare. Only 2 such cases, one in acute monocytic leukemia and one in chronic myelomonocytic leukemia have been described in the literature to date. Here, we report the first case of a 42 year old man with erythroleukemia displaying a pentasomy 8 clone.

Electrophoretic analysis of urinary proteins in diabetic adolescents.

Pathological changes in the urine sodium dodecyl sulphate gel electrophoresis (SDS PAGE) patterns often precede the occurrence of any sign of renal involvement in diabetes. However, data concerning the most frequent SDS PAGE pattern of the urine in early stages of type I diabetes mellitus are controversial. In the present study an SDS PAGE technique has been used that provides an adequate sensitivity for the detection of the abnormal pattern.

Phosphorylation of laminin-1 by protein kinase C.

Several extracellular proteins have been reported to be phosphorylated. Previous studies of our laboratory indicated that laminin-1 can be phosphorylated by protein kinase A (PKA). Moreover, it has been reported that protein kinase C (PKC), although known to be intracellular, can phosphorylate extracellular proteins in the case of cellular damage and/or platelet activation.

Stability of serum TSH concentration after non refrigerated storage.

Background: The ignorance of how sensitive serum samples for defined assays may be, can lead to overprotecting measures. This attitude not only increases cost and labor in everyday routine but may be responsible for not evaluating series of measurements if performed in serum samples not well stored.

Methods: Thyrotropin (TSH) concentration in serum was tested in three pooled samples drawn from various patients, before and after four days storage at freezing (-15 degrees C), low (4 degrees C), and room temperature (18-22 degrees C).

Stability of serum TSH concentration after non refrigerated storage.

Background: The ignorance of how sensitive serum samples for defined assays may be, can lead to overprotecting measures. This attitude not only increases cost and labor in everyday routine but may be responsible for not evaluating series of measurements if performed in serum samples not well stored.

Methods: Thyrotropin (TSH) concentration in serum was tested in three pooled samples drawn from various patients, before and after four days storage at freezing (-15 degrees C), low (4 degrees C), and room temperature (18-22 degrees C).