Publications by authors named "Gaia Poggi"
Context: Maturity-onset diabetes of the young (MODY) 8 is a rare form of monogenic diabetes characterized by a mutation in CEL (carboxyl ester lipase) gene, which leads to exocrine pancreas dysfunction, followed by β cell failure. Induced pluripotent stem cells can differentiate into functional β cells. Thus, β cells from MODY8 patients can be generated in vitro and used for disease modelling and cell replacement therapy.
View Article and Find Full Text PDFBackground Aims: Induced pluripotent stem cells (iPSCs) have the capacity to generate β cells in vitro, but the differentiation is incomplete and generates a variable percentage of off-target cells. Single-cell RNA sequencing offers the possibility of characterizing the transcriptional dynamics throughout differentiation and determining the identity of the final differentiation product.
Methods: Single-cell transcriptomics data were obtained from four stages across differentiation of iPSCs into β cells and from human donor islets.
Article Synopsis
- Brucellosis, caused by Brucella bacteria, is an infectious disease affecting animals, particularly ruminants like water buffaloes, and can lead to economic losses in animal production.
- The study aimed to identify specific microRNA (miRNA) profiles in the blood and vaginal fluids of water buffaloes infected with Brucella, using advanced sequencing and validation methods.
- Results indicated that 20 miRNAs were affected by the infection, showing promise as potential biomarkers for diagnosing Brucella infection and advancing our understanding of immune responses related to the disease.