Comparative study of exoelectrogenic utilization preferences and hydrogen conversion among major fermentation products in microbial electrolysis cells.

This study comparatively investigated the exoelectrogenic utilization and hydrogen conversion of major dark fermentation products (acetate, propionate, butyrate, lactate, and ethanol) from organic wastes in dual-chamber microbial electrolysis cells (MECs) alongside their mixture as a simulated dark fermentation effluent (DFE). Acetate-fed MECs showed the highest hydrogen yield (1,465 mL/g chemical oxygen demand), near the theoretical maximum yield, with the highest coulombic efficiency (105%) and maximum current density (7.9 A/m), followed by lactate-fed, propionate-fed, butyrate-fed, mixture-fed, and ethanol-fed MECs.

A comprehensive analysis of key factors influencing methane production from CO using microbial methanogenesis cells.

With increasing attention on carbon capture and utilization (CCU) technologies for the conversion of CO into chemical products, microbial methanogenesis cells (MMCs) have been extensively studied over the past few decades for biomethane production. Using rapidly accumulating data for MMCs with varying configurations and operating conditions, a comprehensive analysis was conducted here to investigate the critical factors that influence methane production rates (MPR) in these systems. A comparison of MPR and set potentials or current densities showed weak linear relationships (R < 0.

A study of electron source preference and its impact on hydrogen production in microbial electrolysis cells fed with synthetic fermentation effluent.

Fermentation effluents from organic wastes contain simple organic acids and ethanol, which are good electron sources for exoelectrogenic bacteria, and hence are considered a promising substrate for hydrogen production in microbial electrolysis cells (MECs). These fermentation products have different mechanisms and thermodynamics for their anaerobic oxidation, and therefore the composition of fermentation effluent significantly influences MEC performance. This study examined the microbial electrolysis of a synthetic fermentation effluent (containing acetate, propionate, butyrate, lactate, and ethanol) in two-chamber MECs fitted with either a proton exchange membrane (PEM) or an anion exchange membrane (AEM), with a focus on the utilization preference between the electron sources present in the effluent.

Potential treatment of aged cow manure using spare capacity in anaerobic digesters treating a mixture of food waste and pig manure.

With the increasing production of cow manure (CM) and the continuing decrease in the demand for manure compost, CM management has become an urgent and challenging task in Korea. In most cattle farms in Korea, CM mixed with bedding materials is left in pens exposed to the open air for several months before treatment, which makes CM an unsuitable feedstock for anaerobic digestion. This study examined the co-digestion of aged CM with a mixture of food waste and pig manure as the base substrate to assess the possibility of treating and valorizing CM using spare capacity in existing anaerobic digesters dealing with other wastes.

High-rate microbial electrosynthesis using a zero-gap flow cell and vapor-fed anode design.

Microbial electrosynthesis (MES) cells use renewable energy to convert carbon dioxide into valuable chemical products such as methane and acetate, but chemical production rates are low and pH changes can adversely impact biocathodes. To overcome these limitations, an MES reactor was designed with a zero-gap electrode configuration with a cation exchange membrane (CEM) to achieve a low internal resistance, and a vapor-fed electrode to minimize pH changes. Liquid catholyte was pumped through a carbon felt cathode inoculated with anaerobic digester sludge, with humidified N gas flowing over the abiotic anode (Ti or C with a Pt catalyst) to drive water splitting.

Pilot scale microbial fuel cells using air cathodes for producing electricity while treating wastewater.

Microbial fuel cells (MFCs) can generate electrical energy from the oxidation of the organic matter, but they must be demonstrated at large scales, treat real wastewaters, and show the required performance needed at a site to provide a path forward for this technology. Previous pilot-scale studies of MFC technology have relied on systems with aerated catholytes, which limited energy recovery due to the energy consumed by pumping air into the catholyte. In the present study, we developed, deployed, and tested an 850 L (1400 L total liquid volume) air-cathode MFC treating domestic-type wastewater at a centralized wastewater treatment facility.

Vapor-Fed Cathode Microbial Electrolysis Cells with Closely Spaced Electrodes Enables Greatly Improved Performance.

Hydrogen can be electrochemically produced in microbial electrolysis cells (MECs) by current generated from bacterial anodes with a small added voltage. MECs typically use a liquid catholyte containing a buffer or salts. However, anions in these catholytes result in charge being balanced predominantly by ions other than hydroxide or protons, leading to anode acidification.

The impact of different types of high surface area brush fibers with different electrical conductivity and biocompatibility on the rates of methane generation in anaerobic digestion.

The addition of electrically conductive materials may enhance anaerobic digestion (AD) efficiency by promoting direct interspecies electron transfer (DIET) between electroactive microorganisms, but an equivalent enhancement can also be achieved using non-conductive materials. Four high surface area brush materials were added to AD reactors: non-conductive horsehair (HB) and polyester (PB), and conductive carbon fiber (CB) and stainless steel (SB) brushes. Reactors with the polyester material showed lower methane production (68 ± 5 mL/g COD) than the other non-conductive material (horsehair) and the conductive (graphite or stainless steel) materials (83 ± 3 mL/g COD) (p < 0.

Designing a marine outfall to reduce microbial risk on a recreational beach: Field experiment and modeling.

A marine outfall can be a wastewater management system that discharges sewage and stormwater into the sea; hence, it is a source of microbial pollution on recreational beaches, including antibiotic resistant genes (ARGs), which lead to an increase in untreatable diseases. In this regard, a marine outfall must be efficiently located to mitigate these risks. This study aimed to 1) investigate the spatiotemporal variability of Escherichia coli (E.

Addition of a carbon fiber brush improves anaerobic digestion compared to external voltage application.

Two methods were examined to improve methane production efficiency in anaerobic digestion (AD) based on adding a large amount of surface area using a single electrically conductive carbon brush, or by adding electrodes as done in microbial electrolysis cells (MECs) to form a hybrid AD-MEC. To examine the impact of surface area relative to electrodes, AD reactors were fitted with a single large brush without electrodes (FB), half a large brush with two electrodes with an applied voltage (0.8 V) and operated in closed circuit (HB-CC) or open circuit (HB-OC) mode, or only two electrodes with a closed circuit and no large brush (NB-CC) (equivalent to an MEC).

Treatment of Cattle Manure by Anaerobic Co-Digestion with Food Waste and Pig Manure: Methane Yield and Synergistic Effect.

The management of cattle manure (CM) has become increasingly challenging because its production continues to rise, while the regulations on manure management have become increasingly stringent. In Korea, most farms produce CM as a dry mixture with lignocellulosic bedding materials (mainly sawdust), making it impractical to treat CM by anaerobic digestion. To address this problem, this study examined whether anaerobic co-digestion with food waste (FW) and pig manure (PM) could be an effective approach for the treatment of CM.

Individual and combined effects of magnetite addition and external voltage application on anaerobic digestion of dairy wastewater.

Direct interspecies electron transfer (DIET) between exoelectrogenic fatty acid oxidizers and electrotrophic methanogens plays an important role in keeping the overall anaerobic digestion (AD) process well-balanced. This study examined the individual and combined effects of two different DIET-promoting strategies, i.e.

Development of biocathode during repeated cycles of bioelectrochemical conversion of carbon dioxide to methane.

Functioning biocathodes are essential for electromethanogenesis. This study investigated the development of a biocathode from non-acclimated anaerobic sludge in an electromethanogenesis cell at a cathode potential of -0.7V (vs.

A long-term study on the effect of magnetite supplementation in continuous anaerobic digestion of dairy effluent - Magnetic separation and recycling of magnetite.

Promotion of direct interspecies electron transfer (DIET) between exoelectrogenic bacteria and electron-utilizing methanogens has recently been discussed as a new method for enhanced biomethanation. This study evaluated the effect of magnetite-promoted DIET in continuous anaerobic digestion of dairy effluent and tested the magnetic separation and recycling of magnetite to avoid continuous magnetite addition. The applied magnetite recycling method effectively supported enhanced DIET activity and biomethanation performance over a long period (>250days) without adding extra magnetite.

A long-term study on the effect of magnetite supplementation in continuous anaerobic digestion of dairy effluent - Enhancement in process performance and stability.

Interspecies electron transfer (IET) between microbial populations with different functions is critical to stable anaerobic digestion. This study, in an attempt to facilitate IET, investigated the effect of magnetite supplementation on the biomethanation of dairy effluent in continuous mode. The magnetite-added reactor (RM) was significantly more resistant and resilient to process imbalance than the reactor run without magnetite addition (RC).

Anaerobic co-digestion of spent coffee grounds with different waste feedstocks for biogas production.

Proper management of spent coffee grounds has become a challenging problem as the production of this waste residue has increased rapidly worldwide. This study investigated the feasibility of the anaerobic co-digestion of spent coffee ground with various organic wastes, i.e.

Bioaugmentation of anaerobic sludge digestion with iron-reducing bacteria: process and microbial responses to variations in hydraulic retention time.

Although anaerobic digestion (AD) is a widely used option to manage waste activated sludge (WAS), there are some drawbacks related to its slow reaction rate and low energy productivity. This study examined an anaerobic WAS digester, augmented with an iron-reducing microbial consortium, relative to changes in microbial community structure and process performance at decreasing hydraulic retention times (HRTs) of 20 to 10 days. The enhanced methanation performance (approximately 40 % increase in methane yield) by the bioaugmentation was sustained until the HRT was decreased to 12.

Mild-temperature thermochemical pretreatment of green macroalgal biomass: Effects on solubilization, methanation, and microbial community structure.

The effects of mild-temperature thermochemical pretreatments with HCl or NaOH on the solubilization and biomethanation of Ulva biomass were assessed. Within the explored region (0-0.2M HCl/NaOH, 60-90°C), both methods were effective for solubilization (about 2-fold increase in the proportion of soluble organics), particularly under high-temperature and high-chemical-dose conditions.

The biostimulation of anaerobic digestion with (semi)conductive ferric oxides: their potential for enhanced biomethanation.

The effect of biostimulation with ferric oxides, semiconductive ferric oxyhydroxide, and conductive magnetite on the anaerobic digestion of dairy wastewater was examined in a batch mode. The reactors supplemented with ferric oxyhydroxide (R2) and magnetite (R3) showed significantly enhanced biomethanation performance compared with the control (R1). The removal of chemical oxygen demand (COD) after 30 days was 31.

Influence of ferric oxyhydroxide addition on biomethanation of waste activated sludge in a continuous reactor.

This study investigated the potential of enhancing the activity of iron-reducing bacteria (IRBs) to increase the biomethanation rate of waste activate sludge (WAS). The effects of biostimulation by ferric oxyhydroxide (Phase 2) and bioaugmentation with an enriched IRB consortium (Phase 3) were examined in a continuous anaerobic reactor treating WAS. Compared to the control operation (Phase 1), significant rises in methane yield (10.