Beta adrenergic stimulation of protein carboxymethylation and amylase secretion in rat parotid gland.

Protein carboxymethylase (S-adenosyl-l-methionine:protein-O-methyltransferase, EC 2.1.1.

[Identification of actinomycetes with antifungal activity which do not affect Rhizobium meliloti].

Thirteen isolates of actinomycetes that have broad antifungal activity and do not affect two efficient strains of Rhizobium meliloti were identified as: Nocardia autotrophica, Streptomyces antimycoticus, S. anulatus, S. capoamus, S.

Enzymatic methylation of carboxyl groups of chromaffin granule membrane proteins.

Carboxyl groups of membrane and soluble proteins from bovine adrenal medulla chromaffin granules were enzymatically methylated. The methylated peptides were resolved using gel electrophoresis under acidic conditions in the presence of N-cetylpyridinium chloride. There was a selective methylation of two groups of membrane peptides which did not correspond to any of the chromaffin granule soluble proteins.

[Actinomycetes antagonistic to fungi and not affecting Rhizobium meliloti].

The effects of 481 actinomycetes isolated from agricultural soils supporting good growth of alfalfa or clover on two efficient strains of Rhizobium meliloti A2 and S14 were studied. Strain A2 was inhibited by 28% of the isolates and strain S14 was inhibited by 31% of them. No significant difference was found between the resistance of both actinomycete strains.

Selective induction of tyrosine hydroxylase and dopamine beta-hydroxylase by nerve growth factor: comparison between adrenal medulla and sympathetic ganglia of adult and newborn rats.

Administration of NGF to newborn and adult rats elicits a selective increase in TH and DBH both in sympathetic ganglia and adrenal medulla. This effect does not depend on intact preganglionic cholinergic fibers. The augmented enzyme activity results from enhanced enzyme synthesis since it can be abolished by cycloheximide and NGF has been shown to enhance the incorporation of [3H]leucine into DBH molecules.

Synthesis, subcellular distribution and turnover of dopamine beta-hydroxylase in organ cultures of sympathetic ganglia and adrenal medullae.

The synthesis, subcellular distribution and turnover of dopamine beta-hydroxylase was studied in organ cultures of rat adrenal medullae and superior cervical ganglia. After exposure to [3H]leucine for 1 or 3 h, the tissues were homogenized at various time intervals and the amount of labelled dopamine beta-hydroxylase in different subcellular fractions (cytosol, soluble and membrane-bound fraction of catecholamine storage vesicles) was determined by immunoprecipitation and subsequent electrophoresis. In cultured adrenal medullae, induction of dopamine beta-hydroxylase initiated in vivo by administration of reserpine affected both soluble and membrane-bound pools of dopamine beta-hydroxylase to a similar extent after pulse-labelling for 1 or 3 h.

Selective uptake and retrograde axonal transport of dopamine-beta-hydroxylase antibodies in peripheral adrenergic neurons.

In the present experiments the uptake and retrograde axonal transport of antibodies to dopamine beta-hydroxylase (DBH) in adrenergic neurons was studied. When partially purified labelled antibodies to DBH were injected unilaterally into the vicinity of the adrenergic nerve terminals in the iris, radioactive substances accumulated preferentially in the superior cervical ganglia of the injected. By SDS (sodium dodecyl sulfate) gel electrophoresis and immunoprecipitation it could be shown that the accumulated radioactivity in the superior cervical ganglion represented antibodies to DBH.