Long-term monitoring of constructed wetlands in distilleries in Scotland - Evaluating treatment performance and seasonal microbial dynamics.

Constructed wetlands (CWs) have been used to treat industrial wastewater in Scotland, including within the whisky industry to treat the second distillation byproduct known as "spent lees". However, despite the notable advantages of CWs (including simplicity, cost-effectiveness, and energy efficiency) their adoption by distilleries still remains relatively limited. Concerns regarding unstable performance, clogging, and sensitivity to cold temperatures have tended to limit their widespread utilisation.

Bacteria-Produced Algicide for Field Control of Toxic Dinoflagellates Does Not Cause a Cortisol Stress Response in Two Estuarine Fish Species.

Application of algicides produced by naturally occurring bacteria is considered an environmentally friendly approach to control harmful algal blooms. However, few studies assess the effects of bacterial algicides on non-target species, either independently or with other stressors. Here, we measured sub-lethal effects of dinoflagellate-specific algicide IRI-160AA on the estuarine fish Fundulus heteroclitus and Menidia menidia in laboratory experiments.

Analysis of nuclear receptor expression in head and neck cancer.

Objective: Studies of squamous cell carcinoma of the head and neck (HNSCC) have demonstrated the importance of nuclear receptors and their associated coregulators in the development and treatment of HNSCC. We sought to characterize members of the nuclear receptor super family through interrogation of RNA-Seq and microarray data.

Materials And Methods: TCGA RNA-Seq data within the cBioportal platform comparing HNSCC samples (n = 515 patients with RNA-Seq data) to normal tissue (n = 82 patients) was interrogated for significant differences in nuclear receptor expression.

Exactly defined molecular weight poly(ethylene glycol) allows for facile identification of PEGylation sites on proteins.

PEGylation (the covalent attachment of one or more poly(ethylene glycol) (PEG) units to a therapeutic) is a well-established technique in the pharmaceutical industry to increase blood-residence time and decrease immunogenicity. A challenging aspect of PEGylation is the dispersity of PEGylation agents, which results in batch-to-batch variations and analytical limitations. Herein, we present an approach to overcome these limitations by manufacturing a defined molecular weight (dispersity-free) PEGylation agent.

Antioxidant Therapies in the Treatment of Aminoglycoside-Induced Ototoxicity: A Meta-Analysis.

Objective: A feared complication of aminoglycoside treatment is ototoxicity, which is theorized to be attributed to the production of aminoglycoside-induced reactive oxygen species. Previous studies using animal models have suggested that numerous therapies targeting reducing oxidative stress may prevent ototoxicity from aminoglycosides. However, few clinical studies have been conducted on these antioxidants.

Inequality in agricultural greenhouse gas emissions intensity has risen in rural China from 1993 to 2020.

Reducing greenhouse gas (GHG) emissions in crop production while ensuring emission equity is crucial for sustainable agriculture in China, yet long-term large-scale data on GHG emissions intensity (GEI) are limited. Using an extensive dataset based on surveyed farm households (n > 430,000 households) from 1993 to 2020, we reveal that 2015 was a turning point for GEI levels, which dropped 16% in 2020, while inequality-measured as average GHG emissions per unit planted area-increased 13%. The key driving forces behind such trends included farmland input, all other inputs, agricultural labour input and total factor productivity but not capital input.

Elevating water table reduces net ecosystem carbon losses from global drained wetlands.

Drained wetlands are thought to be carbon (C) source hotspots, and rewetting is advocated to restore C storage in drained wetlands for climate change mitigation. However, current assessments of wetland C balance mainly focus on vertical fluxes between the land and atmosphere, frequently neglecting lateral carbon fluxes and land-use effects. Here, we conduct a global synthesis of 893 annual net ecosystem C balance (NECB) measures that include net ecosystem exchange of CO, along with C input via manure fertilization, and C removal through biomass harvest or hydrological exports of dissolved organic and inorganic carbon, across wetlands of different status and land uses.

A comparison between constructed wetland substrates: Impacts on microbial community and wastewater treatment.

Constructed wetlands (CWs) can play a crucial role in treating wastewater, and in the context of this study, the distillation byproduct of the whisky industry known as 'spent lees'. Here, we assess several different CW substrates (pea gravel, LECA and Alfagrog), with and without the addition of 20% biochar, in mesocosms set up to treat spent lees. Among the substrates tested, LECA + biochar and gravel + biochar showed promising results, with greater dissolved copper (dissCu) reduction, chemical oxygen demand (COD) removal, organic carbon (OC) reduction, and pH modulation.

Variants in the autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland.

Fine mapping and bioinformatic analysis of the genetic risk association in Sjögren's Disease (SjD) and Systemic Lupus Erythematosus (SLE) identified five common SNPs with functional evidence in immune cell types: rs4938573, rs57494551, rs4938572, rs4936443, rs7117261. Functional interrogation of nuclear protein binding affinity, enhancer/promoter regulatory activity, and chromatin-chromatin interactions in immune, salivary gland epithelial, and kidney epithelial cells revealed cell type-specific allelic effects for all five SNPs that expanded regulation beyond effects on and expression. Mapping the local chromatin regulatory network revealed several additional genes of interest, including .

Water quality effects of peat rewetting and leftover conifer brash, following peatland restoration and tree harvesting.

Harvesting of plantation conifers on peatlands is carried out as part of restoration and forestry operations. In particular, in the UK and Ireland, conifer plantations on drained ombrotrophic blanket and raised bogs are increasingly being removed (by harvesting), along with blocking of drainage ditches to help raise water tables to reinitiate and restore bog vegetation and function. However, both tree harvesting and peatland restoration operations can have significant impacts on water quality at local and catchment scales.

Comparing DNA isolation and sequencing strategies for 16S rRNA gene amplicon analysis in biofilm containing environments.

Bacteria are primarily responsible for biological water treatment processes in constructed wetland systems. Gravel in constructed wetlands serves as an essential substrate onto which complex bacterial biofilms may successfully grow and evolve. To fully understand the bacterial community in these systems it is crucial to properly isolate biofilms and process DNA from such substrates.

A RETROSPECTIVE EVALUATION OF MORBIDITY AND MORTALITY IN ASSOCIATION WITH INFESTATION BY THE MITE IN AN AVIARY-HOUSED POPULATION OF GOULDIAN FINCHES ().

The Rhynonyssid mesostigmatic mite, , is a well-documented endoparasitic hematophagous arthropod of the respiratory tracts of multiple avian species, particularly Estrildid finches and canaries. In this retrospective study, 175 medical and 278 pathology records for the Gouldian finch () population (N = 377) at the San Diego Zoo between 2013 and 2021 were analyzed to evaluate the effectiveness of ivermectin-based prophylaxis. A multivariable negative binomial regression model was constructed to evaluate the population effects of monthly treatments on morbidity or mortality associated with respiratory mites.

Massively parallel reporter assay confirms regulatory potential of hQTLs and reveals important variants in lupus and other autoimmune diseases.

We designed a massively parallel reporter assay (MPRA) in an Epstein-Barr virus transformed B cell line to directly characterize the potential for histone post-translational modifications, i.e., histone quantitative trait loci (hQTLs), expression QTLs (eQTLs), and variants on systemic lupus erythematosus (SLE) and autoimmune (AI) disease risk haplotypes to modulate regulatory activity in an allele-dependent manner.

Impacts of large-scale climatic circulation on floods through precipitation and temperature in the Lancang-Mekong River Basin.

The importance of large-scale climatic circulation impacts on precipitation and floods in the Lancang-Mekong River Basin (LMRB) has been widely acknowledged. However, the mechanisms related to the impacts of circulation on floods are not yet fully understood. To address this issue, circulations were characterized by using the climate indices, and floods were represented by the flood volume and simulated using an improved hydrological-hydrodynamic model.

Comparison of the three-dimensional chromatin structures of adolescent and adult peripheral blood B cells: implications for the study of pediatric autoimmune diseases.

Background/purpose: Knowledge of the 3D genome is essential to elucidate genetic mechanisms driving autoimmune diseases. The 3D genome is distinct for each cell type, and it is uncertain whether cell lines faithfully recapitulate the 3D architecture of primary human cells or whether developmental aspects of the pediatric immune system require use of pediatric samples. We undertook a systematic analysis of B cells and B cell lines to compare 3D genomic features encompassing risk loci for juvenile idiopathic arthritis (JIA), systemic lupus (SLE), and type 1 diabetes (T1D).

Massively Parallel Reporter Assay Confirms Regulatory Potential of hQTLs and Reveals Important Variants in Lupus and Other Autoimmune Diseases.

Objective: To systematically characterize the potential for histone post-translational modifications, i.e., histone quantitative trait loci (hQTLs), expression QTLs (eQTLs), and variants on systemic lupus erythematosus (SLE) and autoimmune (AI) disease risk haplotypes to modulate gene expression in an allele dependent manner.

Value of DermaCheckup as a novel dedicated digital health solution for teledermatology.

Daily healthcare is becoming increasingly costly and resource-intensive, requiring vast human and financial resources. The primary aim of the present study was to present the initial findings regarding the diagnostic accuracy of a novel telemedicine platform, DermaCheckup, when compared with face-to-face clinical appointments. The secondary aim was to assess whether patient management plans produced via this telemedicine platform differ from those decided upon following a face-to-face dermatological consultation.

Dysregulated long non-coding RNA in Sjögren's disease impacts both interferon and adaptive immune responses.

Objective: Sjögren's disease (SjD) is an autoimmune disease characterised by inflammatory destruction of exocrine glands. Patients with autoantibodies to Ro/SSA (SjD) exhibit more severe disease. Long non-coding RNAs (lncRNAs) are a functionally diverse class of non-protein-coding RNAs whose role in autoimmune disease pathology has not been well characterised.

Systemic lupus erythematosus variants modulate the function of an enhancer upstream of .

/A20 is a prominent autoimmune disease risk locus that is correlated with hypomorphic expression and exhibits complex chromatin architecture with over 30 predicted enhancers. This study aimed to functionally characterize an enhancer ∼55 kb upstream of the promoter marked by the systemic lupus erythematosus (SLE) risk haplotype index SNP, rs10499197. Allele effects of rs10499197, rs58905141, and rs9494868 were tested by EMSA and/or luciferase reporter assays in immune cell types.