Antimicrobial Resistance Among Gram-Negative Bacteria Isolated in the Newborn Intensive Care Unit at Abderrezak-Bouhara Hospital of Skikda, Algeria.

This study aimed to determine the epidemiology of gram-negative bacteria (GNB) isolated in the newborn intensive care unit (NICU) population, to assess their antibiotic susceptibility patterns and possible associated risk factors. All neonates admitted to the NICU of Abderrezak-Bouhara hospital (Skikda, Algeria) with a clinical diagnosis of neonatal infections from March to May 2019 were included in the study. The extended-spectrum β-lactamase ESBLs), plasmidic cephalosporinase (pAmpC), and carbapenemases genes were screened by polymerase chain reaction (PCR) and sequencing.

Broad-spectrum antimicrobial activity of wetland-derived sp. ActiF450.

The increased incidence of invasive infections and the emerging problem of drug resistance particularly for commonly used molecules have prompted investigations for new, safe and more effective microbial agents. Actinomycetes from unexplored habitats appear as a promising source for novel bioactive compounds with a broad range of biological activities. Thus, the present study aimed to isolate effective wetland-derived actinomycetes against major pathogenic fungi and bacteria.

Comparative Genomics among Closely Related Strains Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity.

Specialized metabolites are of great interest due to their possible industrial and clinical applications. The increasing number of antimicrobial resistant infectious agents is a major health threat and therefore, the discovery of chemical diversity and new antimicrobials is crucial. Extensive genomic data from spp.

Diversity and antimicrobial activities of Streptomyces isolates from Fetzara Lake, north eastern Algeria.

A total of 125 Streptomyces strains were isolated from an Algerian wetland (Fetzara Lake) and characterized by growth on different culture media. Phylogenetic analyses were carried out by 16S rRNA sequence comparison after PCR amplification using universal primers. Antibacterial bioassays performed by the agar diffusion method enabled us to retain 33 Streptomyces isolates for their activity against two Gram-positive bacteria (Bacillus subtilis and Micrococcus luteus) and one Gram-negative bacteria (Escherichia coli).

Molecular characterisation of carbapenemases in urban pigeon droppings in France and Algeria.

Objectives: The main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons.

Methods: Stool samples were collected from 73 pigeons in two Mediterranean cities, namely Marseille (France) and Annaba (Algeria). Faecal samples were screened by real-time PCR and standard PCR for the presence of carbapenemase-encoding genes.

First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria.

Here we report the isolation of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae from German cockroaches caught in the burn unit of Batna University Hospital in Algeria. Nine of 12 isolates harbored the blaCTX-M-15 ESBL gene. One Enterobacter cloacae isolate belonging to sequence type 528 coexpressed the blaOXA-48, blaCTX-M-15, and blaTEM genes.

[Antimicrobial activity of Actinomycetale isolated from the lagoon in Algeria].

In the aim of the study of the taxonomy and the antimicrobial activity, a strain of actinomycete SM2/2GF which was isolated from sediment of the lagoon El-Mellah which is situated in the city of El-Kala in the Northeast of Algeria, was tested against diverse pathogenic microorganisms and against a Gram-negative bacterium Pseudomonas alcaliphila which was isolated from water of the lagoon El-Mellah. The phenotypic and the molecular characteristics show that the isolate SM2/2GF belongs to the kind Streptomyces. This strain showed an antimicrobial activity against a Gram-negative bacterium Pseudomonas alcaliphila and the positive-Gram bacteria as Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis, Enterococcus faecalis, as well as the yeast Candida albicans.

A non-polyenic antifungal produced by a Streptomyces yatensis strain isolated from Mellah Lake in El Kala, North-East of Algeria.

Objective: This study aimed at describing one actinomycete strain E65 that was isolated from the water of Mellah Lake in El Kala, North-East of Algeria that produces a non-polyenic antifungal.

Materials And Methods: Actinomycetes were isolated from Mellah Lake water and screened for antimicrobial activity. Antimicrobial assays were performed on ISP2 agar.

Non-contiguous finished genome sequence and description of Paucisalibacillus algeriensis sp. nov.

Paucisalibacillus algeriensis strain EB02(T) is the type strain of Paucisalibacillus algeriensis sp. nov., a new species within the genus Paucisalibacillus.

Non-contiguous finished genome sequence and description of Bacillus massilioalgeriensis sp. nov.

Strain EB01(T) sp. nov. is the type strain of Bacillus massilioalgeriensis, a new species within the genus Bacillus.

Rapid identification of Streptomyces isolates by MALDI-TOF MS.

The recent emergence of multidrug-resistant bacteria over the last decade has led to a renewal in the discovery of new antimicrobial drugs. Streptomyces members are practically unlimited sources of new antibiotics. However, the identification of Streptomyces species is difficult and time-consuming.

Emergence of the European ST80 clone of community-associated methicillin-resistant Staphylococcus aureus as a cause of healthcare-associated infections in Eastern Algeria.

Aims: The authors had for aim to assess whether an in-hospital spread of the European community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) clone was on-going in Annaba, Eastern Algeria.

Material And Methods: We carried out a molecular epidemiological study of S. aureus strains causing infections in 4 hospitals located in Annaba, between February and October 2010.