Structural and functional comparison of HemN to other radical SAM enzymes.

Radical SAM enzymes have only recently been recognized as an ancient family sharing an unusual radical-based reaction mechanism. This late appreciation is due to the extreme oxygen sensitivity of most radical SAM enzymes, making their characterization particularly arduous. Nevertheless, realization that the novel apposition of the established cofactors S-adenosylmethionine and [4Fe-4S] cluster creates an explosive source of catalytic radicals, the appreciation of the sheer size of this previously neglected family, and the rapid succession of three successfully solved crystal structures within a year have ensured that this family has belatedly been noted.

An active site homology model of phenylalanine ammonia-lyase from Petroselinum crispum.

The plant enzyme phenylalanine ammonia-lyase (PAL, EC 4.3.1.