The calcium signaling pathway regulates leydig cell steroidogenesis through a transcriptional cascade involving the nuclear receptor NR4A1 and the steroidogenic acute regulatory protein.

In the gonads and adrenal glands, the transient increase in steroidogenesis after hormonal stimulation requires modulation of steroidogenic acute regulatory protein (Star) expression and activity in a tightly regulated process involving cAMP and Ca(2+). In Leydig cells, the cAMP and Ca(2+) pathways account for most if not all of LH-induced steroidogenesis. Although the cAMP-activated molecular network has been well characterized in Leydig cells, little is known about the molecular cascade triggered by the Ca(2+) signaling pathway and the transcription factors responsible for mediating the genomic response.