[Study of sensitivity of microflora isolated from biosubstrates of surgical profile patients to bacteriophage].

Aim: Study sensitivity of nosocomial microbes--causative agents of post-operative infectious complications in surgical profile patients to bacteriophages.

Materials And Methods: Microbe isolates from biosubstrates of 223 surgical patients served as material. Microbes were isolated from blood, urine, wounds, intravascular catheters, abdominal cavity drainage, discharge of pharynx, trachea (343 strains).

[Persistence of Pseudomonas aeruginosa strains in patients of Federal Scientific Center of Transplantology and Artificial Organs].

Aim: Study genetic diversity of P. aeruginosa strains persisting in patients of Federal Scientific Center of Transplantology and Artificial Organs, and main factors facilitating persistence of strains in the hospital.

Materials And Methods: 136 P.

[Genotypic features of Pseudomonas aeruginosa strains circulating in surgical hospital].

Aim: To study genetic diversity of Pseudomonas aeruginosa strains circulating in intensive care unit (ICU), to determine the source of these strains and duration of circulation of epidemically-significant clone in the hospital.

Materials And Methods: Genotyping of 106 P. aeruginosa strains isolated from patients, clinical specimens and fomites was performed by random amplified polymorphic DNA analysis with oligonucleotide primer Sh1 of 10 bp long.

[Transposition of autologous cultivated bone marrow cells promotes prevention and treatment of immunodependent complications in cardiosurgical patients].

Coronary artery bypass grafting (CABG) has been made in two groups of patients with chronic heart failure (CHF) with further estimation of the rate of postoperative organic dysfunctions and pyoseptic complications. In group 1 (n = 50) CABG was combined with intracoronary or intramyocardial injection of autologous precultivated for 7-8 days mononuclear cells of the bone marrow (1 x 10(9) cells). In group 2 (n = 479) the intraoperative injection of the above cells was not made.

[Enterococci as a cause of infectious complications after surgery].

Self-obtained data about study of enterococci as etiologic agents of infectious complications after cardiovascular and transplantation surgery are presented in the article. 465 strains of enterococci were isolated from different biosubstrates. Isolation rate of different species of enterococci in blood, urine, fromwounds, trachea, cardiacvalves, as well as in the environment and on wear of healthcare workers were determined.

[Sensitivity of nosocomial microflora circulating in a transplantation clinic to medicinal bacteriophages].

The sensitivity of 239 isolates obtained from patients with postoperative infectious complications to phagolysis was determined. Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were found to have the highest sensitivity to phages. Variations in the sensitivity of the same cultures to phages from different producers and even from the same producer were established.

[The morphofunctional changes in the hepatocytes of rats with experimental acute pancreatitis].

Under experimental acute pancreatitis of rats, certain changes were revealed in the liver histological structure, hepatocyte population composition, intensity of hepatocyte DNA-synthesis, their proliferation, and in hepatocyte glycogen contents. These changes reflect a two-step process of hepatocyte activation. This results well compares with the earlier evidence on changes in pancreatic cell population under acute pancreatitis.

[Infectious complications following heart transplantation].

The work analyses infectious complications after 35 orthotopic transplantations of the heart. The infectious complications are divided into 2 groups. Group 1 consisted of 6 patients with local complications.

[Phospholipids and glycosphingolipids in cultured skin fibroblasts from healthy donors and patients with systemic scleroderma].

A comparative study of phospho- and glycosphingolipids of cultured skin fibroblast from healthy donors and from patients with systemic sclerodermia (SSD) was carried out. It was shown that the total phospholipid content in SSD fibroblasts is elevated. No significant changes in the concentration of neutral glycosphingolipids were observed.

[A comparative evaluation of various methods for determining the degree of endogenous intoxication in patients with phlegmons of the maxillofacial area].

Comparison of 3 methods for the assessment of the severity of intoxication in patients with maxillofacial phlegmons, i.e. the leukocytic index of intoxication (LII), Paramecium test (PT), and measurement of the middle molecule level (MML), has shown that all these methods sufficiently well reflect the severity of the disease clinical course.

[Morphofunctional changes in the exocrine pancreatic cells in acute experimental pancreatitis in rats].

Experimental pancreatitis was induced by cooling the splenetic part of rat pancreas with chlorethyl, and the cells of duodenal area of the pancreas were studied at different stages of pancreatitis using cytomorphometry, cytomorphology and autoradiography. Interlobular and interacinar oedemas were observed at the first hours after treatment. In 24 hours the intracellular oedema of exocrine pancreatic cells (EP) was detected.

[Endogenous toxemia in acute disorders of cerebral circulation].

Critical conditions that develop because of hypoxia of different genesis are accompanied by endogenous intoxication induced to a considerable degree by the appearance in the blood of an excess amount of medium-sized molecules (MSM). An appreciable increase of the content of MSM was demonstrated since the very first days after the brain stroke as a result of examining 100 patients who suffered brain stroke, including 83 patients during the first week of the disease and 17 patients of the reference group with a history of acute impairment of cerebral circulation which had occurred over a month before. A reverse correlation was established between the level of endogenous intoxication and the clinical status of the patients which could be observed every day during the acute disease phase.

[Cytological research on the action of sodium thiosulfate on the process of induced acute pancreatitis in rats].

The influence of sodium thiosulfate (STS) on the process of experimental acute pancreatitis (EAP) in rats was studied by cytomorphology, morphometry, autoradiography and cytophotometry. The influence was shown to vary at different stages of disease development. At the first stage ("primary effect" state) STS leads to the increase in the stability of exocrine pancreacytes (EP) against the toxins and to the decrease in the activity of proteases formed during necrobiosis.

[Changes in the content of nucleic acids and total protein in exocrine pancreatocytes and the composition of their population in experimental acute pancreatitis in rats].

Using cytophotometry, contents of DNA, RNA and total protein were measured in the rat's exocrine pancreatocytes (EP) in normal conditions and at different stages of pancreatitis induced by cooling the spleen part of the pancreas with chlorethyl. In the duodenal (not damaged) part of the pancreas some drastic changes in the EP ploidy distribution were shown to occur. They led to the formation of a qualitatively new population pattern with 4c and 2c + 2c cells prevailing (more than 60% of the total content), which are by 1.

[A comparative evaluation of the efficacy of methods of determining osmolality and middle molecules in the prognosis of the course of strokes].

The authors compare the efficacies of methods for assessing osmolality (OSM) and analysis of medium molecules (MM) in the prediction of the post-stroke period course in the first 7 days from the stroke onset. Prognostically unfavorable MM and OSM values were found, predicting the possible lethal outcome. On the whole the MM index was found 1.

[Ribonuclease in the experimental therapy of pancreatitis].

The influence of ribonuclease on the morphogenesis of experimental pancreatitis in the albino rats has been studied. The drug injected during edematous stage of pancreatitis caused some decrease of pancreatic enzymes level in the blood at hemorrhagic stage and its normalization at necrotic stage of pancreatitis. The development of hemorrhagic and necrotic stages of pancreatitis did not change under the influence of ribonuclease.

[The role of lipids in middle molecular weight fractions as a feature of the pathological process].

About 20-66% of lipids was found in the middle molecular fraction of blood serum from neurosurgical patients. Content of the substances extracted with organic solvents was distinctly increased as the clinical status aggravated. Precipitation of proteins using chloric acid instead of trichloracetic acid proved to be more convenient for isolation of the middle molecular fraction from blood serum and for the subsequent studies of its composition.

[Glycosylation of glycoconjugates on thymocyte surfaces].

The ectosialation and ectogalactosylation of mouse thymocyte surface were studied. The incorporation of labeled monosaccharides (N-acetylneuraminic acid and galactose) into cell surface glycoproteins and glycolipids were demonstrated. Identification of glycolipids was carried out.

[The level of middle molecules in endogenous intoxication in neurosurgical patients].

The article deals with the results of study of the level of medium-size molecules in blood and urine of neurosurgical patients in the early postoperative period. It correlated with the severity of the patients' condition and with the values of osmolarity and the level of consciousness in a complicated postoperative period and a fatal outcome. The presence of pyoseptic complications may be judged according to the P value and prognosticated.

[Effect of synthetic analogs of L-enkephalin on viable areas of the pancreas in experimental pancreatitis].

Synthetic analogues of L-enkephalin--tageflar and dalargin were studied for the treatment of experimentally-induced pancreatitis of rats. It was concluded that morphometric features of an intact part of the pancreas were not significantly changed with the use of tageflar. The intensity of 14C-leucine inclusion in the proteins of an intact part of the pancreas was strongly suppressed and the cytoplasm of exocrine pancreocytes was overloaded with zymogen granules.

[Diagnosis of septic conditions in newborn infants with surgical diseases].

An analysis of 65 case histories of newborns with pyo-septic surgical diseases has been made. The extraction-spectrometrical method of analysis of blood serum is proposed. The distribution index (DI) in sepsis was always less than 1.