Publications by authors named "Gabriela de Andrade Bruni"
Article Synopsis
- In Experiment 1, PBMCs were collected from healthy and SCE-affected cows on Days 0 and 7 of their estrous cycle to analyze gene expression related to immunity and inflammation.
- In Experiment 2, cows were categorized based on pregnancy status on Day 21 post-artificial insemination, and significant differences in gene expression were found between pregnant and non-pregnant cows, particularly in ISG15 and pro-inflammatory cytokines.
- The results suggest that OAS1 and IL1-β levels increase during diestrus, regardless of SCE, while ISG15 abundance may serve as a reliable indicator of pregnancy success.
Development of new methods for early diagnosis of pregnancy can be important to increase the reproductive efficiency and profitability of dairy herds. The bovine conceptus secretes IFN-τ that stimulates the transcription of several genes in circulating immune cells and extrauterine tissues. The aims of this study were to evaluate the mRNA abundance for pregnancy predictability of a classic gene stimulated by IFN-τ () and a novel potential pregnancy marker () in peripheral blood mononuclear cells (PBMC), total blood leukocytes (TBL) or milk leukocytes (TML), and cervical cells (CC) on d 20 after timed artificial insemination (TAI) in dairy cattle.
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