Western blotting (immunoblotting): history, theory, uses, protocol and problems.

Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues. The history surrounding the origin of western blotting, the theory behind the western blotting technique, a comprehensive protocol and the uses of western blotting are presented. Lesser known and significant problems in the western blotting field and troubleshooting of common problems are highlighted and discussed.

Intra-abdominal Pressure Screening of Horses With Colic.

The objective of this study was to evaluate changes in intra-abdominal pressure (IAP) in horses with colic by associating the underlying etiologies with directly acquired IAP values and survival rate. This is a 2-year cohort study (2014-2016). Horses with clinical signs of colic were admitted to the veterinary teaching hospital during the period 2014-2016.

Intrabladder pressure as predictor of intra-abdominal pressure in horses.

Objectives: To investigate effects of postural changes and bladder distention on intrabladder pressure whilst estimating intra-abdominal pressure in horses.

Design: Two-year cohort study. Patients admitted for elective surgical procedures unrelated to gastrointestinal or genitourinary tract.

After Sexual Identity Disclosure: An Ecological Perceptive of LGB Young Adults.

Objectives A plethora of research exists concerning determinants prior or during the sexual identity disclosure process; yet, information is limited concerning internal and external experiences after one discloses his or her sexual identity to their social support system. Through the lenses of the Social Ecological Model, the purpose of this study was to further understand the current lived experiences of persons in post-sexual identity disclosure. Methods Semi-structured interviews were conducted among 15 lesbian, gay, and bisexual young adults.

Anaphylactoid reaction caused by sodium ceftriaxone in two horses experimentally infected by Borrelia burgdorferi.

Background: Lyme borreliosis is a disease transmitted by ticks to mammals, especially in horses and humans. Caused by a spirochete Borrelia burgdorferi, it can result in lameness, arthritis, carditis, dermatitis and neurological signs. Anaphylactoid reactions are severe responses caused by direct action of substances (drugs, toxins), which can pose risks to life.