Evaluation of Cytotoxicity, Cell Attachment, and Elemental Characterization of Three Calcium Silicate-Based Sealers.

We investigated three calcium silicate-based sealers with respect to their chemical characterization, cytotoxicity, and attachment to RAW264.7 cells. BioRoot RCS (BR), Bio-C Sealer (BC), and Sealer Plus BC (SPBC) were assessed using Fourier transform infrared spectroscopy (FTIR), X-ray fluorescence spectroscopy (XRF), and energy-dispersive X-ray spectroscopy (EDX) (n = 4) for elemental characterization, and using scanning electron microscopy (SEM) to evaluate cell morphology and adhesion.

Effects of different calcium-silicate based materials on fracture resistance of immature permanent teeth with replacement root resorption and osteoclastogenesis.

Objectives: This study evaluated the effects of Biodentine (BD), Bio-C Repair (BCR), and mineral trioxide aggregate (MTA) plug on the fracture resistance of simulated immature teeth with replacement root resorption (RRR) and -induced osteoclastogenesis.

Materials And Methods: Sixty bovine incisors simulating immature teeth and RRR were divided into 5 groups: BD and BCR groups, with samples completely filled with the respective materials; MTA group, which utilized a 3-mm apical MTA plug; RRR group, which received no root canal filling; and normal periodontal ligament (PL) group, which had no RRR and no root canal filling. All the teeth underwent cycling loading, and compression strength testing was performed using a universal testing machine.

Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production.

Objective: This study aimed to evaluate the antibacterial activity of crude Brazilian red propolis (BRP) extract against anaerobic bacteria involved in primary endodontic infection. Additionally, we evaluate the cell viability and free radical production of human dental pulp fibroblasts (HDPF) in direct contact with mineral trioxide aggregate (MTA) and BRP.

Design: The Minimum Inhibitory Concentration, Minimum Bactericidal Concentration (MIC, MBC) and Minimum Inhibitory Concentration of Biofilm (MICB) of BRP against anaerobic endodontic pathogens were determined.

Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells.

Objectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm).

Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm and 80 mW/cm) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm shortly after LPS supplementation.

Biological parameters, discolouration and radiopacity of calcium silicate-based materials in a simulated model of partial pulpotomy.

Aim: To analyse the discolouration, radiopacity, pH and calcium ion release of Biodentine (BD), Bio-C repair (BCR) and Bio-C temp (BCT), as well as their biological effects on human dental pulp cells (hDPCs).

Methodology: Sixty-four extracted bovine incisors were prepared to simulate crown fractures with pulp exposure and open root apex. The roots were filled using a mixture of agar and blood (control), and BD, BCR or BCT were placed over this mixture.

Effects of zinc oxide and calcium-doped zinc oxide nanocrystals on cytotoxicity and reactive oxygen species production in different cell culture models.

Objectives: This study aimed to synthesize nanocrystals (NCs) of zinc oxide (ZnO) and calcium ion (Ca)-doped ZnO with different percentages of calcium oxide (CaO), to evaluate cytotoxicity and to assess the effects of the most promising NCs on cytotoxicity depending on lipopolysaccharide (LPS) stimulation.

Materials And Methods: Nanomaterials were synthesized (ZnO and ZnO:xCa, x = 0.7; 1.

Cytotoxicity and Effects of a New Cacium Hydroxide Nanoparticle Material on Production of Reactive Oxygen Species by LPS-Stimulated Dental Pulp Cells.

Introduction: The aim of this study was to synthesize and characterize calcium hydroxide (CH) nanoparticles [CH-NP] and compare the cytotoxicity of these materials with that of mineral trioxide aggregate (White MTA) in human dental pulp mesenchymal cells (hDPMCs) stimulated by lipopolysaccharide (LPS).

Methods And Materials: The CH-NP were synthesized by the co-precipitation method, and the physical properties were investigated through X-ray diffraction, scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). LPS-stimulated hDPMCs were placed in contact with different dilutions of culture media previously exposed to CH-NP and white MTA for 24 h.

Effects of Lectin (ScLL) on osteoclast-like multinucleated giant cells' maturation-A preliminary in vitro study.

Background/aim: Lectin (ScLL) has been recently evaluated in the oral cavity due to its anti-inflammatory activities. ScLL could be a promising agent for blocking osteoclast activity and preventing root resorption. The aim of this study was to evaluate the effect of ScLL on the viability of the RAW 264.

[International Nursing Minimum Data Set: a comparative study with tools of a pediatric clinic].

Descriptive and comparative study, developed from two historical used to implement the Systematization of Nursing Care in a Pediatric clinic of a teaching hospital. It aimed to compare the International Nursing Minimum Data Set (i-NMDS) with the data contained in nursing clinical assessment tools that are used in the application of the Nursing Process in the area of Children and Teenagers Health in a Pediatric Clinic. From the sixteen items evaluated that belong to the proposal of the i-NMDS, the nursing clinical assessment tool for hospitalized teenagers included 12 (75%), while the instrument for children from 0 to 5 years old presented 7 (43.

[Sexuality of the elderly: behavior for the prevention of STD/AIDS].

This study deals with the theme "elders facing sexually transmitted diseases (STDs)" and it aims to identify the behavior of the elderly in the prevention of STD/AIDS. It is a qualitative, exploratory and descriptive study using a semi-structured questionnaire with open questions. The study subjects were six seniors who participated in a group in a Basic Health Unit.