Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus.

The objective of the present study was to evaluate the antimicrobial activity of the -LTP2 and -GRP peptides isolated from seeds and their possible synergistic activity with the azole drug fluconazole and characterize their mechanisms of action on cells of pathogenic fungi. -LTP2 and -GRP alone or in combination with 20 g/mL of fluconazole were evaluated for their antimicrobial activity on the fungus , and the effects of these peptides on the permeability of membranes and the induction of oxidative stress were determined. Our results show that these peptides at a concentration of 400 g/mL combined with 20 g/mL of fluconazole were able to inhibit the growth of the tested fungi, promote changes in their growth pattern, permeabilize the membrane, and induce reactive oxygen species (ROS).