The Effects of Prednisone/Ketoprofen Administration in Association with Amoxicillin Clavulanate Following Periodontal Surgical Therapy in Patients with Severe Chronic Periodontitis.

: The aim of this study was to evaluate and compare the effects of two different anti-inflammatory drugs (ketoprofen and prednisone) combined with an antibiotic (amoxicillin + clavulanic acid) and periodontal surgery on dental and periodontal parameters in patients with severe chronic periodontitis. In addition, salivary stress expressed by cortisol levels was assessed. : An interventional study was performed on 22 periodontal subjects and 19 clinical healthy controls.

Study of antimicrobial effects of functionalized silver nanoparticles.

In recent decades, the study of nanoparticles (NPs) has gained a great scientific interest, due to a wide range of potential applications, in different fields: electronic, optical and biomedical. Some of the most studied effects of NPs are antibacterial ones, because the large, sometimes unjustified and uncontrolled administration of antibiotics has led to the emergence of multidrug-resistant (MDR) bacterial strains. In our study, starting from silver nitrate (AgNO3), we made approximately 30 nm spherical AgNPs that were coated with a thin layer of ethylene glycol (EG) or EG and polyvinylpyrrolidone (PVP).

Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections.

The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence and prevalence of invasive infections in a hospital environment.

Immunohistochemistry predictive markers for primary colorectal cancer tumors: where are we and where are we going?

The aim of our study is to highlight and organize the recently published immunohistochemistry (IHC) predictive biomarkers of primary colorectal cancers (CRCs) that could lead to practical implementation. We reviewed articles that examined CRC samples with significant statistic correlation between the IHC marker expression and disease progression over time, relationships with the available clinical features and those who detect the prognosis of drug effects. Our analysis showed that nine markers could correlate with medical treatment response of CRCs in different stages.

Design, Synthesis, Characterization and Antimicrobial Evaluation of Some Heterocyclic Condensed Systems with Bridgehead Nitrogen from Thiazolotriazole Class.

In the present study, a series of new heterocyclic condensed systems with bridgehead nitrogen from the thiazolo[3,2-b][1,2,4]triazoles class was synthesized starting from some 4-(4-X-phenylsulfonyl)phenyl)-4H-1,2,4-triazole-3-thioles 1a-c (X=H, Cl, Br). The intermediates of S-alkylated 1,2,4-triazoles, 2-(5-(4-(4-X-phenylsulfonyl)phenyl)-2H-1,2,4-triazol-3-ylthio)-1-(4-fluorophenyl)ethanones 2a-c, were obtained by treatment of triazoles 1a-c with 2-bromo-4'-fluoroacetophenone. The 2-(4-(4-X-phenylsulfonyl)phenyl)-6-(4-fluorophenyl)thiazolo[3,2-b][1,2,4]triazoles 3a-c were obtained by cyclization of S-alkylated 1,2,4-triazoles 2a-c in sulfuric acid media, at 0 °C.

Antibiotic susceptibility of 33 Prevotella strains isolated from Romanian patients with abscesses in head and neck spaces.

The purpose of this study was to investigate the susceptibility of a series of 33 Prevotella strains isolated from patients with abscesses in the head and neck spaces, presented to one Romanian hospital. The Etest was applied to determine the value of the minimum inhibitory concentrations for: penicillin G, ampicillin, amoxicillin-clavulanate, metronidazole and clindamycin. In addition, the beta-lactamase activity was detected by the chromogenic cephalosporin disc method.

Susceptibility testing of Streptococcus pneumoniae and Viridans streptococcal isolates against: quinolones, oxazolidinones and glycopeptides.

Unlabelled: The aim of this study was to test the susceptibility of 100 strains (pneumococci and viridans streptococci) isolated from oral and respiratory tract infections and their complications, against one antibiotic of each of the following classes: quinolones, oxazolidinones and glycopeptides.

Material And Methods: The Etest has been used in order to investigate the susceptibility of the isolates against levofloxacin, linezolid and vancomycin.

Results And Conclusions: As expected, the results of the study indicated that all isolates were susceptible to linezolid and vancomycin.

New heterocyclic compounds from 1,2,4-triazole and 1,3,4-thiadiazole class bearing diphenylsulfone moieties. Synthesis, characterization and antimicrobial activity evaluation.

Some new 5-(4-(4-X-phenylsulfonyl)phenyl)-4-(R)-2H-1,2,4-triazol-3(4H)-thiones 4a,b; 5a,b and 5-(4-(4-X-phenylsulfonyl)phenyl)-N-(R)-1,3,4-thiadiazol-2-amines 6a,b; 7a,b were obtained by cyclization of new N(1)-[4-(4-X-phenylsulfonyl)benzoyl]-N(4)-(R)-thiosemicarbazides 2a,b; 3a,b (X=H, Br). The 1,2,4-triazoles were synthesized by intramolecular cyclization of acylthiosemicarbazides, in basic media. On the other hand, 1,3,4-thiadiazoles were obtained from same acylthiosemicarbazides, in acidic media.

Synthesis and antimicrobial evaluation of some fused heterocyclic [1,2,4]triazolo[3,4-b][1,3,4]thiadiazole derivatives.

A series of fused 1,2,4-triazoles with diphenylsulfone moiety are prepared utilizing 4-amino-5-[4-(4-X-phenylsulfonyl)phenyl]-4H-1,2,4-triazole-3-thiol 1 (X=H, Br). The latter on reaction with aromatic isothiocyanate in DMF, aromatic acid in POCl3 and CDI in dioxane gives five membered fused triazole derivatives 2a-c, 3a-c, 4a-g, 5a-g and 6a,b. The structures of newly synthesized compounds were confirmed on the basis of their elemental analysis and spectral data results (IR, 1H-and 13C NMR).

Contribution towards developing a new semi-selective medium for Streptococcus anginosus group.

Unlabelled: The aim of this study was to develop a new selective medium for isolation of Streptococcus anginosus group (SAG) strains, by adding sulphamethazine and aztreonam as selective agents at Mitis-Salivarius agar (MSA), the medium commonly used for recovery of oral streptococci from oral samples.

Material And Method: The evaluation of Mitis-Salivarius--sulphamethazine--aztreonam agar (MSSAA) for SAG selectivity was performed by testing the growth of type strains and laboratory-stored clinical isolates of different oral streptococcal species on this medium and also by investigating the SAG recovery on MSSAA in comparison with MSA and the growth inhibition of non-SAG strains from 100 saliva and 11 pus samples (collected from healthy young subjects and from paediatric patients with upper respiratory tract infections, respectively) on MSSAA.

Results: The same SAG recovery rate was obtained on both MSSAA and MSA, while non-SAG strains failed to grow on the novel medium, except for enterococci.

Synthesis, characterization and evaluation of antibacterial activity of some thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties.

A series of thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties were synthesized starting from 5-[4-(4-X-phenylsulfonyl)phenyl]-4H-1,2,4-triazole-3-thioles 3a-c, X=H, Cl, Br. Thus, alkylation of 1,2,4-triazoles 3 with phenacyl bromide or 4-bromophenacyl bromide afforded S-substituted 1,2,4-triazoles 4, 5. These new intermediates 4 and 5, in the presence of H(2)SO(4) (c), were cyclized to 2-[4-(4-X-phenylsulfonyl)phenyl]-6-(4-Y-phenyl)[1,3]thiazolo[3,2-b]-[1,2,4]-triazoles 6, 7 (I) and not to isomeric thiazolo[2,3-c][1,2,4]-triazoles 6, 7 (II).

[Anaerobic Gram-negative bacilli involved in the etiopathogeny of the abscesses of superficial fascial spaces of the face and neck].

Unlabelled: The aim of this study was to isolate and identify at species level the strains of anaerobic Gram-negative bacilli (GNB) from pus samples collected in patients with abscesses of fascial spaces of the face and neck. Microscopy of Gram-stained smears and cultures were performed in each specimen. The strictly anaerobic GNB strains were identified using the conventional methods of diagnosis and the Rapid ID 32 A system.

Oral streptococcal strains isolated from odontogenic infections and their susceptibility to antibiotics.

The aim of this study was to identify at species level and to investigate the antibiotic susceptibility of oral streptococcal strains isolated from 100 pus samples collected from Romanian patients with different odontogenic infections. The isolates were identified at species level using the Rapid ID 32 STREP system and their susceptibility was testing by the Etest, against: penicillin G, ampicillin, erythromycin, clindamycin and tetracycline. For the investigation of erythromycin resistance phenotype the disk diffusion test was used.

[Epidemiologic aspects of the edentulous mouth (II). General and local features of the totally edentulous mouth].

Unlabelled: PREMISES AND AIM: The study wants to reveal the general and local clinical aspects, which may accompany the completely edentulous mouth and create difficulties in treatment.

Material And Method: The clinical and statistical study was carried out on 40 edentulous patients; we analyzed general aspects (the present medical status, the patient's medication and its effects) and local aspects (the etiology, the type of previous denture treatment, the type and number of denture's repairs, the extent of the residual ridge resorption, the relationship between residual ridges), the total difficulty of the treatment.

Results And Discussions: 80% of the edentulous patients suffered from general diseases, more frequent from cardiovascular, digestive, metabolic, renal, rheumatic diseases.

[Phenotypical characterization of some strains of anginosus streptococci isolated from superficial face and neck abscesses].

The aim of the present study was to identify at species level and to characterize the strains of streptococci belonging to anginosus group isolated from 30 pus samples collected from patients with abscesses of fascial spaces of the face or neck. The identification of facultatively anaerobic Gram-positive cocci in chains at genus level was made by conventional diagnosis methods and at species level by the help of Rapid ID 32 STREP system. The cultural, biochemical and antigenic (limited at Lancefield Group identification by Slidex Strepto-Kit) characterization was performed in the mean time with the species identification.

[Prevotella species involved in the onset of superficial face and neck abscesses].

The aim of the study was to identify at species level the Prevotella strains from 30 pus samples collected by needle aspiration in patients with abscesses of fascial spaces of the face and neck. Microscopy of Gram-stained direct smear and cultures on selective and nonselective media incubated aerobically and anaerobically were performed in each pus specimen. All strains of Gram-negative strictly anaerobic bacilli were identified to genus and species level using conventional methods of diagnosis and the Rapid ID 32 A system (BioMérieux, Marcy-l'Etoile, France).

[Epidemiologic aspects of the totally edentulous mouth. (1) General aspects].

Unlabelled: PREMISES AND AIM: This study analyses the evolution of the populational segment represented by edentulous patients, specific to our country, regarding the number of edentulous patients, related to age, sex.

Material And Method: We performed two statistical studies on edentulous patients in 1994 and in 2004 and we compared their results. In order to evaluate the present clinical aspects of the edentulous patients, we performed this year a clinical study on 40 patients, which were classified according to their age and sex.