Publications by authors named "Gabriel L A Silva"
Unpicking the Roles of DNA Damage Protein Kinases in Trypanosomatids.
Front Cell Dev Biol
August 2021
Article Synopsis
- Cells have a complex system known as the DNA damage response (DDR) that activates to fix DNA damage through various repair pathways, with protein kinases (PKs) playing a key role in this process.
- Research on model eukaryotes has revealed how these PKs regulate DNA repair and their connections to other cellular activities like DNA replication and transcription.
- Kinetoplastid parasites, such as some that cause neglected tropical diseases, show unique DNA repair mechanisms and have raised questions about the roles of certain kinases, presenting opportunities for new treatments using kinase inhibitors.
Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene deletion to show that HUS1, a component of the 9-1-1 (RAD9-RAD1-HUS1) complex, is essential and is required for a G2/M checkpoint.
View Article and Find Full Text PDFA DiCre recombinase-based system for inducible expression in Leishmania major.
Mol Biochem Parasitol
September 2017
Here we present the establishment of an inducible system based on the dimerizable Cre recombinase (DiCre) for controlled gene expression in the protozoan parasite Leishmania. Rapamycin-induced DiCre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. The DiCre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system.
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