[Molecular mechanism of the action of lucanthone on tumor and normal cells].

The standard analytical and preparative electrophoresis in polyacrylamide gel did not reveal qualitative differences in the fraction composition of c-RNA of the ascitic tumour cells (Zaidel hepatoma, Ehrlich carcinoma, NK/ly lymphoma) and normal liver cells. In vitro the tumour and normal cells efficiently incorporated the labeled uridine into the all main classes of c-RNA (4S, 18S, 28S, fractions above 28S), the process being more intensive in the tumour cells. The presence of tumours in the animal organism had a significant effect on RNA biosynthesis in the liver, the effect being dependent on the tumor nature.

[Geycogen content and the enzymatic system of its biosynthesis in normal and tumorous human tissues].

In human tumors (cancer of the liver, stomach, lymphogranulomatosis of lymphnodes, metastases in lymphnodes, ascites ovarian tumor cells) the authors studied the content of enzymes participating in glycogen biosynthesis through uridine diphosphateglucose mechanism--UDPG-glycogensynthetase, UDPG-pyrophosphroylase, phosphoglucomutase. As a rule, in human tumor cells the amount of glycogen and the activity of enzymes of its biosynthesis is decreased, the more the higher proliferative activity and autonomization of tumor cells.

[Advantages of the Eagle medium over saline solutions in the studies of the metabolic parameters of tumor cells in vitro].

In two media (krebsringer phosphate and phosphate-enriched Eagle medium) the indices of energetic metabolism (respiration, glycolysis. ATP content) were determined and the metabolism of macromolecular compounds (c-RNA) of Ehrlich carcinoma ascitic cells was investigated. The results of studies of ascites tumor cells metabolism have indicated considerable advantages of phosphate-enriched Eagle medium over krebs ringer phosphate buffer.

[Content of the components of the adenylic acid system in solid and ascitic tumors in the liver of experimental animals].

The authors report the results of separate determination of the concentration of free adenine nucleotides (ATP, ADP, AMP) in tumors, intact animals liver, and tumor-bearing animals liver. In Zajdela ascites hepatoma, ascites tumor NKly and solid lymphosarcoma, solid hepatomas 46 and 22 A the amount of ATP and ADP was found to be markedly reduced compared with their content in the liver. The ratio ATP/ADP is increased in ascites cells of tumor NKly, Zaidela hepatoma and lymphosarcoma and is decreased in solid hepatoma 46 and 22 A.

[Fractional composition of the RNA of mouse Krebs-II ascitic cell tumor and rat ovarian carcinoma].

Main fractions (28 S, 18 S and 4S RNA) of cytoplasmic and nuclear RNA were isolated preparatively from two variants of ascites tumor cells i. e. Krebs-11 carcinoma in mice and ovarian tumor in rats.

[Glycogen and enzymes involved in its biosynthesis in rat liver during liver carcinogenesis caused by diethylnitrosamine].

An attempt was made to correlate variations in the level of glycogen during chemically induced carcinogenesis (DENA) with changes in the activity of enzymes participating in biosynthesis of this polysaccharide (UDPG-glycosensynthetase, phosphoglucomutase, UDPG-pyrophosphorylase). It is found that in DENA action in the rat liver two phases may be sharply differentiated--initial (the 1st month) with marked alterations in the content of glycogen and the activity of related enzymes, and final one (4-6th month) which is characterized by reduction of glycogen content, a new sharp rise in the activity of phosphoglucomutase and UDPG-pyrophosphorylase, and somewhat decreased activity of UDPG-glycogensynthetase, as compared with the initial values.