Boron-containing antibacterial agents: effects on growth and morphology of bacteria under various culture conditions.

The effects of a number of synthetic antibacterial agents of the borinic acid and diazaborine types on the growth of enteric bacteria were examined. In minimal medium aerobic growth was immediately slowed; slow nonexponential growth continued for an extended period, and the cells remained viable. The effect was also seen in anaerobic cultures and was not antagonized by a number of common nutrients, vitamins, or growth factors.

Isolation and structure of nocobactin NA, a lipid-soluble iron-binding compound from Nocardia asteroides.

Nocobactin NA, a lipid-soluble iron-chelating product with an unusual and characteristic u.v.-absorption spectrum, was isolated from Nocardia asteroides grown under conditions of iron deficiency.

Chemical and biological properties of mycobactins isolated from various mycobacteria.

Nine different strains of mycobacteria grown on media deficient in iron all produced mycobactins. Most strains produced one mycobactin in great preponderance. Mycobacteria from clearly distinct taxonomic groups gave mycobactins differing in the structure of their nuclei.

Metal complexes of mycobactin P and of desferrisideramines.

Crystalline gallium mycobactin P and chromic mycobactin P have been prepared. The chromic compound, unlike other metallic complexes of mycobactin P, does not detectably exchange its metal with ferric iron; it competitively antagonizes the growth-promoting action of mycobactin P towards Mycobacterium johnei. Mycobactin P, desferrioxamine B and desferrichrysin form coloured 1:1 complexes with ammonium vanadate.

Isolation of mycobactinss from various mycobacteria. The properties of mycobactin S and H.

Mycobactin S has been isolated from Mycobacterium smegmatis and from Mycobacterium sp. Olitzky & Gershon, strain 2, and mycobactin H from M. thermoresistible; all three organisms were grown on synthetic media of low iron content.

Methods for the separation and identification of mycobactins from various species of mycobacteria.

A single pure component was isolated from mycobactin P by countercurrent distribution; its side chain is n-cis-octadec-2-enoyl; its purity and molecular structure were confirmed by mass spectrometry of its aluminium complex. The separation of ferric and of aluminium complexes of mycobactins by thin-layer chromatography is described. Mycobacterium terrae, M.

Assay of the mycobactins by measurement of the growth of Mycobacterium johnei.

A method is described for the assay of mycobactin P and T by turbidimetric measurement of the growth of Mycobacterium johnei. The assay agrees satisfactorily with spectroscopic determination of the iron complexes. The growth curves with mycobactins P and T differ somewhat in form.

Isolation and structure of mycobactin T, a growth factor from Mycobacterium tuberculosis.

1. A growth factor for Mycobacterium johnei has been isolated from Mycobacterium tuberculosis as an iron complex; it has been named ferric mycobactin T. 2.

THE STRUCTURE OF MYCOBACTIN P, A GROWTH FACTOR FOR MYCOBACTERIUM JOHNEI, AND THE SIGNIFICANCE OF ITS IRON COMPLEX.

1. The growth factor from Mycobacterium phlei is now named mycobactin P to distinguish it from related but chemically distinct growth factors from other species of mycobacteria. It is shown to comprise four closely similar chemical entities differing only in the fatty side chain.

Metabolism and excretion of di(p-aminophenyl) sulphoxide in different animal species.

Rabbits, rats and guinea-pigs were treated with di(p-aminophenyl) sulphoxide and their urines examined by an analytical method which permits the simultaneous determination of this compound and of dapsone [di(p-aminophenyl) sulphone] which is a possible product of metabolic oxidation. The method gives for each drug the total of free compound plus acid-labile conjugates. All three species excreted unchanged drug together with dapsone.