Whole-Exome Sequencing Identifies Novel GATA5/6 Variants in Right-Sided Congenital Heart Defects.

One out of every hundred live births present with congenital heart abnormalities caused by the aberrant development of the embryonic cardiovascular system. The conserved zinc finger transcription factor proteins, which include GATA binding protein 5 (GATA5) and GATA binding protein (GATA6) play important roles in embryonic development and their inactivation may result in congenital heart defects (CHDs). In this study, we performed genotypic-phenotypic analyses in two families affected by right-sided CHD diagnosed by echocardiography imaging.

Prevalence of Comorbid Hyperparathyroidism and Its Association with Renal Dysfunction in Asian Patients with X-Linked Hypophosphatemic Rickets/Osteomalacia.

In patients with X-linked hypophosphatemic rickets/osteomalacia (XLH) in Asia, the current prevalence of hyperparathyroidism and its association with renal dysfunction have not been determined. We used patient data retrospectively collected up to the time of informed consent in the SUNFLOWER study, a long-term observational study, to investigate the current treatment status and prevalence of comorbid hyperparathyroidism and its association with renal dysfunction in patients with XLH in Japan and South Korea. Of 69 patients who met the eligibility criteria, 32 (46.

Modulating the Hydrogen Bond for a Stable Zinc Anode with a Wide Temperature Range via the Sucrose and Polyacrylamide Synergistic Effect.

Zinc-ion batteries become a major research focus in energy storage, valued for their low cost and high safety. However, their widespread application is hindered by poor zinc anode stability caused by dendrites, side reactions, and poor performance across a wide temperature range at a strong hydrogen bond network aqueous electrolyte. In this study, we propose a strategy for the synergistic combination of a polyacrylamide hydrogel with sucrose.

Psychological resilience to trauma and longitudinal sleep outcomes among current and former nurses.

Objective: A life-disrupting stressor (e.g. pandemic) may cause or exacerbate poor sleep health; resilience may offset impacts.

Influenza 5xM2e mRNA lipid nanoparticle vaccine confers broad immunity and significantly enhances the efficacy of inactivated split vaccination when coadministered.

Current influenza vaccines are not effective in conferring protection against antigenic variants and pandemics. To improve cross-protection of influenza vaccination, we developed a 5xM2e messenger RNA (mRNA) vaccine encoding the tandem repeat conserved ectodomain (M2e) of ion channel protein M2 derived from human, swine, and avian influenza A viruses. The lipid nanoparticle (LNP)-encapsulated 5xM2e mRNA vaccine was immunogenic, eliciting high levels of M2e-specific IgG antibodies, IFN-γ+ T cells, T follicular helper cells, germinal center phenotypic B cells, and plasma cells.

Preserving the Biologically Coherent Generic Concept of , 'Plant Destroyer'.

is a long-established, well known and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from at least twice. Since, cladistically, this renders 'paraphyletic', it has been proposed that evolutionary clades be split into multiple genera (Runge et al.

Controllable Preparation of Low-Cost Coal Gangue-Based SAPO-5 Molecular Sieve and Its Adsorption Performance for Heavy Metal Ions.

With the advancement of industrial production and urban modernization, pollution from heavy metal ions and the accumulation of solid waste have become critical global environmental challenges. Establishing an effective recycling system for solid waste and removing heavy metals from wastewater is essential. Coal gangue was used in this study as the primary material for the synthesis of a fully coal gangue-based phosphorus-silicon-aluminum (SAPO-5) molecular sieve through a hydrothermal process.

Phosphorylation of an RNA-Binding Protein Rck/Me31b by Hippo Is Essential for Adipose Tissue Aging.

The metazoan lifespan is determined in part by a complex signaling network that regulates energy metabolism and stress responses. Key signaling hubs in this network include insulin/IGF-1, AMPK, mTOR, and sirtuins. The Hippo/Mammalian Ste20-like Kinase1 (MST1) pathway has been reported to maintain lifespan in Caenorhabditis elegans, but its role has not been studied in higher metazoans.

The mitigation mechanism of morin on PM-induced apoptosis by inhibiting mitochondrial damage via the ROS/ERK signaling pathway in human HaCaT keratinocytes.

Particulate matter 2.5 (PM) can penetrate the human skin, causing inflammatory disease, senescence, and carcinogenesis. This study assessed the effects of morin on PM-induced apoptosis of HaCaT human keratinocytes.

CDK4 selective inhibition improves preclinical anti-tumor efficacy and safety.

CDK4/6 inhibitors have revolutionized treatment of hormone receptor positive (HR+), HER2 non-amplified (HER2-) breast cancer. Yet, all "dual" CDK4/6 inhibitors show common dose-limiting hematologic toxicities, foremost neutropenia. This poses challenges to provide these agents at concentrations necessary to extinguish cell cycling in tumors.

ImmunoPET Imaging of Trop2 Expression in Bladder Cancer Using [Cu]Cu-NOTA-Trodelvy.

Trop2 exhibits significantly elevated expression in numerous solid malignancies, playing a crucial role in tumor advancement, whereas its presence in healthy tissues is minimal. In this study, we investigated Trop2 expression in bladder cancer models using [Cu]Cu-NOTA-Trodelvy for immunoPET imaging. In HT-1376 models, [Cu]Cu-NOTA-Trodelvy effectively visualized tumor as early as 12 h p.

Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root.

A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in Salvia miltiorrhiza Bge. root. In this study, a series of polarity-adjustable DESs were prepared using n-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined.

An innovative high-rate biofilm-based process: Biopolymer production and recovery from wastewater organic pollutants.

In this study, a novel high-rate moving bed biofilm reactor (MBBR) was constructed to enhance wastewater COD bio-conversion and biopolymer recovery with a hydraulic retention time (HRT) of 1.0 h and an organic loading rate (OLR) of 4.8 kg COD·m·d.

Pharmacodynamic target attainment of the synergism of ceftazidime-avibactam in combination with amikacin against OXA-producing extensively drug-resistant or pan drug-resistant (XDR/PDR) Pseudomonas aeruginosa.

To investigate the pharmacodynamic target attainment of ceftazidime-avibactam (CZA) in combination with amikacin against OXA-producing extensively drug-resistant/ pan-drug-resistant Pseudomonas aeruginosa (XDR/PDR-PA). The minimum inhibitory concentrations (MICs) of CZA and amikacin against OXA-producing XDR/PDR-PA were determined by the checkerboard method, and the combined inhibitory index (FICI) was calculated to evaluate whether the combination of the two antimicrobials has a synergistic effect on OXA-producing XDR/PDR-PA in vitro. The pharmacokinetic (PK) and pharmacodynamic (PD) parameters of CZA and amikacin were combined by Monte Carlo simulation (MCS) to evaluate the cumulative fraction of response (CFR) of the two antimicrobials for the treatment of OXA-producing XDR/PDR-PA infection.

Metabolic engineering of Corynebacterium glutamicum for highly selective production of 5-hydroxyvaleric acid.

The biosynthesis of 5-hydroxyvaleric acid (5-HV) from glucose via the l-lysine degradation pathway cocurrently generates by-products, including l-lysine, 5-aminovaleric acid (5-AVA), and glutaric acid (GTA), which are closely interconnected with the 5-HV biosynthesis pathway. This study focuses on developing a highly selective 5-HV production system in Corynebacterium glutamicum. Initial strategies, such as using sorbitol as a co-substrate, deleting the endogenous GTA biosynthesis pathway, and incorporating a GTA recycling system, were insufficient to achieve selectivity.

FeO loaded biochar to enhance persulfate activation for tetracycline degradation: Performance and mechanism.

Waste sodium lignosulfonate (LS) is widely converted into biochar-based catalysts due to its rich functional groups and high carbon content. However, inefficient peroxydisulfate (PDS) activation by original sodium lignosulfonate biochar (LB) limits its ability to remove organic pollutants from water bodies. Here, LS was employed as a precursor to synthesize FeO-loaded biochar (FeO@LB) through a one-step pyrolysis process for activating PDS to remove tetracycline (TC).

Anchoring nanoscale zero-valent iron within bacterial cellulose particles for boosting efficient adsorption of Co(II) and Sr(II) from seawater: Dual system and varying adsorption mechanisms.

Increasing attention has been paid to radioactive wastewater to direct discharge in Japan or accidental leaks. Strontium-90 (Sr) and Cobalt-60 (Co) are the most hazardous nuclides in waste discharged form nuclear reactors. Because of their high solubility and long half-lives, these radioisotopes can persist for hundreds of years before decaying to negligible levels.

Reliability of partial-mouth periodontal examinations for diagnosing periodontal disease.

Purpose: This study investigated the reliability of partial-mouth periodontal examinations (PMPEs) for 1) identifying the presence of periodontitis, 2) staging periodontal disease, and 3) reflecting mean clinical parameters.

Methods: All patients were diagnosed using 8 different exam types: (A) full-mouth periodontal examination (FMPE), (B) Community Periodontal Index of Treatment Needs (CPITN) codes, (C) panoramic radiographs only, (D) clinical parameters only, (E) 6 teeth, including the first molar (#11, #16, #26, #31, #36, #46), (F) CPITN index teeth (#11, #16, #17, #26, #27, #31, #36, #37, #46, #47), (G) Ramfjord teeth (#16, #21, #24, #36, #41, #44), and (H) 10 modified Ramfjord teeth, including all first premolars and first molars (#16, #14, #21, #24, #26, #36, #34, #41, #44, #46). Case definitions were established according to the criteria outlined in the 2018 American Academy of Periodontology/European Federation of Periodontology classification of periodontal diseases.

Dynamic changes of nutrients, isoflavone composition and antioxidant activities during liquid-state fermentation of soybean embryo homogenate by .

This study aims to explore compositional changes of oligosaccharides, amino-type nitrogen, free amino acids volatile compounds, isoflavones, total phenol, total flavonoid, and antioxidant capacities during liquid-state fermentation (LSF) of soybean embryo homogenate (SEH) by to improve usability as functional food material. Raffinose and stachyose were completely hydrolyzed into monosaccharides such as fructose, glucose and galactose after 2 days of fermentation. After 5 days of fermentation, the contents of amino-type nitrogen and total free amino acids increased 7.

Branched-Chain Amino Acids Target miR-203a/fosb Axis to Promote Skeletal Muscle Growth in Common Carp ().

Starvation is an environmental stress that cannot be ignored during the growth of aquatic animals. Amino acid composition and balance can influence the nutritional effects, regulating the anabolic metabolism and energy signaling in the organism. Among these, branched-chain amino acids (BCAAs), which are essential amino acids in fish, play vital roles in energy regulation and growth metabolism.

Screening and Identification of Bacteriophages VC3 and Its Role in the Inhibition and Removal of Biofilms in Seafood.

As biological control agents, bacteriophages can both inhibit the pathogenic bacteria and remove the bacterial biofilms from the seafood. is the pathogen of cholera and the severe infection could lead watery diarrhea and even death. The double-layer agar plate method was used to isolate and screen the bacteriophages from the samples of aquaculture water and sewage.

Dicer Loss in Müller Glia Leads to a Defined Sequence of Pathological Events Beginning With Cone Dysfunction.

Purpose: The loss of Dicer in Müller glia (MG) results in severe photoreceptor degeneration, as it occurs in retinitis pigmentosa or age-related macular degeneration; however, the sequence of events leading to this severe degenerative state is unknown. The aim of this study was to conduct a chronological functional and structural characterization of the pathological events in MG-specific Dicer-conditional knockout (cKO) mice in vivo and histologically.

Methods: To delete Dicer and mature microRNAs (miRNAs) in MG, two conditional Dicer1 knockout mouse strains (Rlbp-CreER:tdTomato:Dicer-cKOMG and Glast-CreER:tdTomato:Dicer-cKOMG) were created.

Effects of pharmacological therapy on sleep quality in a postoperative setting: A systematic review of randomized controlled trials.

Background And Aims: Postoperative sleep disturbances are associated with delayed recovery and increased incidences of complications. This systematic review aims to determine the impact of perioperative pharmacological therapies on postoperative sleep quality in the hospital.

Material And Methods: We searched MEDLINE, MEDLINE ePubs and In-Process Citations (Daily), Embase Classic + Embase, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, and PubMed for randomized controlled trials (RCTs) from inception to May 2022, with continued literature surveillance until August 2023.

A study on the relationship between high school students' sleep quality, physical exercise, academic stress, and subjective well-being.

Objective: This Research explores the intermediary function of academic stress and sleep quality in the connection between physical exercise and subjective well-being among high school students.

Methods: This Research conducted an online survey through random sampling with a sample of 540 (males = 310, females = 217; grade 10 = 159, grade 11 = 190, grade 12 = 178) high school students to explore the intermediary function of academic stress and sleep quality between physical exercise and subjective well-being. The participants were surveyed using PSQI, the Subjective Well-Being Scale, the ESSA, and PSQI.