Response of ZmPHO1 family members to low phosphorus stress and association of natural variation in ZmPHO1;2a reveal the role of low phosphorus tolerance.

Phosphorus (Pi) is an essential nutrient for plants to sustain normal life processes. In this study, we found that the ZmPHO1 proteins had similar molecular weights and the same conserved domain. Phylogenetic and cis-acting element analysis showed that ZmPHO1s were divided into 4 subgroups, in which ZmPHO1;2a and ZmPHO1;2b were closely phylogenetic with OsPHO1;2b, and the promoter region of ZmPHO1s contained abundant abiotic stress-related elements.

"From Waste to Wonder": Comparative Evaluation of Chinese Cabbage Waste and Banana Peel Derived Hydrogels on Soil Water Retention Performance.

Under the increasing severity of drought issues and the urgent need for the resourceful utilization of agricultural waste, this study aimed to compare the soil water retention properties of hydrogels prepared from Chinese cabbage waste (CW) and banana peel (BP) using grafting techniques with acrylic acid (AA) and acrylamide (AAm). Free radical polymerization was initiated with ammonium persulfate (APS), and N, N'-methylene bisacrylamide (MBA) served as the crosslinking agent to fabricate the grafted polymer hydrogels. The hydrogels were subjected to detailed evaluations of their water absorption, reusability, and water retention capabilities through indoor experiments.

Physicochemical property and microbial community characteristics of the casing soil for cultivating .

Background: Casing soil is critical for the cultivation process of and promotes the formation of mushroom fruiting bodies. Therefore, reliable casing soil indicators are crucial for obtaining high yields of high-quality mushrooms.

Methods: In this study, soil enzyme activity, physicochemical properties, and microorganisms at five cultivation stages [namely casing (A1), mycelial (A2), primordial (A3), fruiting (A4), and harvesting (A5)] of cultivation were evaluated in casing soils.

De novo 2'-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis.

2'-Fucosyllactose (2'-FL) is the most abundant human milk oligosaccharide and plays significant roles in gut microbiome balance, neural development, and immunoregulation. However, current fermentation schemes using multiple carbon sources increase production cost and metabolism burden. This study reported the development of an engineered Bacillus subtilis strain that produces 2'-FL using glucose as the sole carbon source.

A multifunctional bacterial cellulose-based dressing modified by quaternized chitosan and grafted protocatechuic acid for diabetic ulcer.

Herein, we developed a multifunctional bacterial cellulose-based dressing (PHBC) modified by quaternized chitosan (HACC) along with protocatechuic acid (PA), through in situ biosynthesis combined with covalent immobilization. The obtained PHBC dressing maintained the excellent physicochemical characteristics of BC, such as high porosity (above 76 %); high water absorption ratio, >80 % of water absorption rate (approximately 30 g/g) has completed in half an hour; favorable hydrophilicity with contact angle of about 50° and excellent flexibility. The introduction of PA-grafted HACC endows exhibited outstanding antibacterial properties against, anti-inflammatory performance and antioxidant capacity.

Biodegradable sodium lignosulfonate-based superabsorbent hydrogels for disposable hygiene products based on hyperbranched polyetherpolyol crosslinkers.

Full bio-degradation of super absorbent hydrogels (SAHs), which possess excellent water uptake capacity, is still challenging. Based on Flory theory, this paper first analyzes that the high swelling property and gel strength of SHAs are attributed to the length of effective chain of crosslinker. Firstly, a series of hyperbranched crosslinker (HBC) with different molecular weights were designed and synthesized by anionic polymerization and sodium lignosulfonate (SL), which owns a hydrophilic network and remarkable biodegradation properties, was selected as a monomer.

Clinical and Genomic Prediction of Coronary Artery Disease Subtypes.

Background: Coronary artery disease (CAD) is a complex, heterogeneous disease with distinct etiological mechanisms. These different etiologies may give rise to multiple subtypes of CAD that could benefit from alternative preventions and treatments. However, so far, there have been no systematic efforts to predict CAD subtypes using clinical and genetic factors.

Engineering Halomonas bluephagenesis for synthesis of polyhydroxybutyrate (PHB) in the presence of high nitrogen containing media.

The trade-offs exist between microbial growth and bioproduct synthesis including intracellular polyester polyhydroxybutyrate (PHB). Under nitrogen limitation, more carbon flux is directed to PHB synthesis while growth is inhibited with diminishing overall carbon utilization, similar to the suboptimal carbon utilization during glycolysis-derived pyruvate decarboxylation. This study reconfigured the central carbon network of Halomonas bluephagenesis to improve PHB yield theoretically and practically.

Hydrophobic bio-based foam prepared from recycled pulp fiber and its properties.

Compared with traditional petroleum-based foam materials, cellulosic foam materials have significant advantages in terms of economy and environmental protection. However, the traditional cellulose-based foams have some problems such as high energy consumption in the preparation process. In this study, the recycled pulp foam (RPF) with ultra-light density (12.

Nonalkaline Fabrication of Al-Based Metal-Organic Frameworks with Tailored Water Sorption Properties via Polymeric Hydroxy-Aluminum Basicity Modulation.

Metal-organic frameworks (MOFs) are porous crystalline materials composed of metallic nodes and organic ligands, demonstrating increasing potential in water harvesting in arid and semiarid regions. This study presents a nonalkaline, water-based, and scalable synthesis strategy designed to adjust the water sorption properties of aluminum-based MOFs (Al-MOFs), specifically, AlFum and MOF-303, by modifying the basicity of the metal source, polymeric hydroxy-aluminum, as an alternative. Characterizations, including X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric analyses (TGA), confirmed the successful synthesis of Al-MOFs.

Enhancement and mechanism of mechanical properties and functionalities of polyacrylamide/polyacrylic acid hydrogels by 1D and 2D nanocarbon.

Highly flexible hydrogels are widely used in fields such as agriculture, drug delivery, and tissue engineering. However, the simultaneous integration of excellent mechanical properties, swelling properties, and high electrical conductivity into a hydrogel is still a great challenge. This work introduces 1D tubular multi-walled carbon nanotubes (MWCNTs) and 2D layered graphene oxide (GO) into polyacrylamide/poly-acrylic acid (PAM/PAA) hydrogels.

Accumulation of 3-Monochloro-Propanediol Esters in Kidney Tissues of Patients with Human Renal Cell Carcinoma.

Background: 3-Monochloro-propanediol esters (3-MCPDEs), commonly found in refined edible oils and related products, have generated concerns due to their nephrotoxicity and carcinogenicity, yet clinical evidence remains limited.

Objectives: In this study, we aimed to assess, for the first time, the accumulation of 3-MCPDEs in human kidney tissues, focusing on 68 participants, some with and others without renal cell carcinoma (RCC).

Methods: An analytical method for 3-MCPDE determination in kidney tissues underwent partial validation to ensure its suitability for sample analysis.

Transforming watermelon rind into durable superabsorbent hydrogels for enhanced soil water retention properties and adsorbs dye in water.

Innovative superabsorbent hydrogels were synthesized from watermelon rind (WR), an abundant agricultural waste. The process involved free radical polymerization of acrylic acid (AA) and acrylamide (AAm) with WR particles activated by ammonium persulfate (APS), resulting in (AA-co-AAm)/WR hydrogels with high equilibrium swelling capacities of 749 ± 32 g/g. Notably, after eight cycles, the WR hydrogel maintained 94.

Mechanism of enhanced salt tolerance in Saccharomyces cerevisiae by CRZ1 overexpression.

Achieving high-gravity fermentation in the industrial production of fuel ethanol, and enhancing the fermentation efficiency of high-salt raw materials, such as waste molasses, can significantly reduce wastewater output and process costs. Therefore, the development of hyperosmotic-tolerant industrial Saccharomyces cerevisiae strains, capable of resisting high-salt stress, offers both environmental and economic benefits. Our previous study highlighted the potential of CRZ1 overexpression as a strategy to improve the yeast strain's resistance to high-salt stress, however, the underlying molecular mechanisms remain unexplored.

Identification of key hub genes in knee osteoarthritis through integrated bioinformatics analysis.

Knee osteoarthritis (KOA) is a common chronic joint disease globally. Synovial inflammation plays a pivotal role in its pathogenesis, preceding cartilage damage. Identifying biomarkers in osteoarthritic synovial tissues holds promise for early diagnosis and targeted interventions.

Myeloid clonal hematopoiesis of indeterminate potential in patients with chronic lymphocytic leukemia.

Clonal hematopoiesis of indeterminate potential (CHIP) in patients with chronic lymphocytic leukemia (CLL) has not been extensively characterized. The objective of this study was to describe the prevalence of myeloid CHIP (M-CHIP) in patients with CLL, and to determine its association with time to first treatment (TTFT) and overall survival (OS). We retrospectively analyzed data from patients participating in a prospective CLL database at the Dana-Farber Cancer Institute who had standard-of-care targeted 95-gene next-generation sequencing (NGS) performed.

All-Arthroscopic Treatment of Combined Off-Track Hill-Sachs Lesions Using Interference Screw and Shoulder Glenoid Bone Defects Using Bone Grafting With Soft Fixation.

Bony changes such as glenoid bone defects and Hill-Sachs lesions are responsible for recurrent anterior shoulder dislocations. With the development of arthroscopic techniques as well as arthroscopic surgical instruments, arthroscopic repair of bony structures has become an important surgical procedure for the treatment of recurrent shoulder dislocation. In this Technical Note, we used screws to fill Hill-Sachs lesions and autologous iliac bone grafts combined with soft tissue to repair the glenoid bone defects.

Synthesis and characterization of pH-responsive sodium alginate/humic acid composite hydrogels for sustained drug release of l-ascorbic acid.

Addressing the challenges of poor biocompatibility and degradability of artificial drug carriers and poor stability of natural monomer gels in the current pharmaceutical market, this study utilized green natural sodium alginate (SA) and humic acid (HA) as raw materials. SA/HA-Ca hydrogel spheres were prepared using Ca as a crosslinking agent and loaded with l-ascorbic acid (L-AA), and the drug-retardation study was conducted using the droplet method. Various analysis techniques confirmed the formation of a three-dimensional network structure through coordination between HA, SA, and Ca, resulting in hydrogel spheres with good thermal stability.

Clinical performance of a particle enhanced turbidimetric immunoassay (PETIA) for detecting fecal calprotectin.

Particle-enhanced turbidimetric immunoassay (PETIA) is a new measurement procedure for detecting fecal calprotectin (FC). We aimed to investigate the accuracy and clinical performance of PETIA for FC. We assessed the accuracy of PETIA for FC measurements through concordance analysis, Passing-Bablok regression and Bland-Altman analysis, using enzyme-linked immunosorbent assay (ELISA) as the reference.

Analysis of the gut microbiota and fecal metabolites in people living with HIV.

The gut microbiome has a pivotal function in human immunodeficiency virus (HIV). However, the associated alterations in the gut microbiome-host interaction are unknown. Herein, we aimed to investigate the gut microbiota and fecal metabolites in people living with HIV (PLWH).

Design of Mixed-Metal MOF-74-MgNi for Water Adsorption-Driven Solar Thermal Energy Storage and Heat Transformation Applications.

Adsorption solar thermal energy storage and heat transformation are ecologically benign and energy-efficient technologies. Efficient adsorbents are the key to this technology. In this paper, two metal ions, Mg and Ni, were introduced into the metal-organic framework MOF-74.