Adriamycin causes up regulation of epidermal growth factor receptors in actively growing cells.

We have demonstrated a drug-dependent increase in the capacity of HeLa and 3T3 cells, grown in the presence of lethal and sublethal concentrations of adriamycin, to bind epidermal growth factor (EGF). Scatchard analysis ascribes this effect to an increase in the number of binding sites, with little change in affinity. The time course of binding of 125I-EGF is unchanged by adriamycin treatment, in both 3T3 and HeLa cells, at both 0 and 37 degrees C.

Genetic and physiological tests of three phosphate-specific transport mutants of Escherichia coli.

Phosphate-specific transport system mutations phoT35, pst-2, and phoS25-(Am) were mapped between bgl and glmS, at about 83 min on the Escherichia coli chromosome. All three mutations were recessive to wild-type genes on transducing bacteriophage lambda asn. The phoS25 (Am) and pst-2 mutations were also recessive to transducing phage lambda dglm; however, the phoT35 mutation was not.

Pleiotropic effects of alkaline phosphatase regulatory mutations phoB and phoT on anaerobic growth of and polyphosphate synthesis in Escherichia coli.

The appearance during anaerobiosis of spontaneous phoT phoB double mutants in a phoT background is described. During both exponential growth and stationary phase, selection against the phoT mutants relative to the wild type was evident. This reduction in viability of phoT mutants was suppressed in phoT phoB double mutants.