Genetic Heterogeneity of MET-Aberrant NSCLC and Its Impact on the Outcome of Immunotherapy.

Introduction: Robust data on the outcome of MET-aberrant NSCLC with nontargeted therapies are limited, especially in consideration of the heterogeneity of MET-amplified tumors (METamp).

Methods: A total of 337 tumor specimens of patients with MET-altered Union for International Cancer Control stage IIIB/IV NSCLC were analyzed using next-generation sequencing, fluorescence in situ hybridization, and immunohistochemistry. The evaluation focused on the type of MET aberration, co-occurring mutations, programmed death-ligand 1 expression, and overall survival (OS).

Systemic inflammatory changes after pulmonary vein radiofrequency ablation do not alter stem cell mobilization.

Aims: Aim of this study was to investigate the number of circulating progenitor cells, systemic inflammatory mediators, and myocardial necrosis in patients with paroxysmal atrial fibrillation (AF) undergoing pulmonary vein (PV) isolation by radiofrequency (RF) ablation. Radiofrequency ablation generates a localized myocardial necrosis that might result in a release of inflammatory mediators enhancing progenitor cell mobilization and improving tissue repair.

Methods And Results: Blood samples were collected in patients with paroxysmal AF before and after PV isolation.

[The cell response of rat esophagus after intraluminal irradiation with Nd-YAG-laser (1064 nm) after various postoperative times: a light and electron microscopic study].

The esophagi of Wistar rats were irradiated by a Nd-YAG-laser and studied by light and transmission electron microscopy immediately, 2 days and 14 days after the operation. In the immediate group the lasercentre showed a destruction of the stratified epithelium. On the contrary the cells and fibres of the underlying connective tissue were hardly affected.

Effect of sulfur dioxide inhalation on the respiratory tract of the rat.

In order to study the initial effect of SO2 exposure by electron microscopy, Wistar rats were subjected to the gas for 8 hours at a concentration of 800 ppm. A gradient of decreasing damage was observed in the tracheobronchial tree in peripheral direction. The trachea epithelium showed the most severe lesions represented by groups of detached cells, necrotic cells and disappearance of cilia and goblet cells.

[The morphological results of the use of a pulsed krypton fluoride excimer laser for enamel conditioning].

Although there have been many advances in the field of acid etching, major problems still present. For the most part, these are the unpredictability of the effects of etching, the lack of control of the depth of etching over the long term, and the development of enamel fractures occurring on debonding. The aim of this study was to investigate the morphological effects of enamel conditioning using a pulsed krypton fluoride excimer laser (wavelength 248 nm).