Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. II. Comments on the methods and type classification tests of E. rhusiopathiae strains.

The tube precipitation method with antigen produced from autoclaved organisms and those produced by CH3COOH extraction and with good antisera have proved to give the best results and should be preferred at least in the routine work. It is a quick method but the results cannot be fixed and later used in documentation. - The paper chromatographic method is very sensitive, makes much work and heavy demands on the clarity of the antigen and serum and reading the test.

Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. I. Methods and influence of some factors on their results.

To contribute to the standardization of typing Erysipelothrix rhusiopathiae strains the following points have been examined and compared: the usefulness of tube precipitation, paper chromatography and agar gel diffusion; different methods to produce good antisera (specific high titres) and antigens (CH3COOH-, HCl-extract, extraction by means of autoclavation). Rabbits have a considerably varying individual ability to produce Erysipelas antibodies with good precipitation titres. The antigenic effect of different E.