Publications by authors named "G W Barnard"
Article Synopsis
- Targeted integration (TI) in CHO cell lines is a method used for protein expression, but the effect of epigenetic modifications on these cells hasn't been thoroughly investigated.
- This study focused on analyzing various TI clones with identical transgene copy numbers but different levels of protein expression and found that chromatin accessibility, rather than histone modifications, plays a crucial role in influencing transcription efficiency.
- Attempts to enhance protein production by modifying histone profiles at the CMV promoters did not yield significant improvements, suggesting that increasing chromatin accessibility is key for boosting mRNA transcription and overall productivity in TI cell lines.
Article Synopsis
- Clinical whole-genome sequencing (WGS) has the potential to improve treatment for children with cancer and has been integrated into routine testing across two medical centers.
- In a study of 281 children, WGS altered management in about 7% of cases and provided additional clinically relevant genomic information in nearly 30% of instances.
- The findings show that WGS not only replicates standard molecular tests but also uncovers new genomic features, highlighting its effectiveness in tailored patient care.
Chinese hamster ovary (CHO) cells are the preferred system for expression of therapeutic proteins and the majority of all biotherapeutics are being expressed by these cell lines. CHO expression systems are readily scalable, resistant to human adventitious agents, and have desirable post-translational modifications, such as glycosylation. Regardless, drug development as a whole is a very costly, complicated, and time-consuming process.
View Article and Find Full Text PDFChinese hamster ovary (CHO) cells are the predominant host of choice for recombinant monoclonal antibody (mAb) expression. Recent advancements in gene editing technology have enabled engineering new CHO hosts with higher growth, viability, or productivity. One approach involved knock out (KO) of BCAT1 gene, which codes for the first enzyme in the branched chain amino acid (BCAA) catabolism pathway; BCAT1 KO reduced accumulation of growth inhibitory short chain fatty acid (SCFA) byproducts and improved culture growth and titer when used in conjunction with high-end pH-controlled delivery of glucose (HiPDOG) technology and SCFA supplementation during production.
View Article and Find Full Text PDFArticle Synopsis
- - The study aimed to evaluate the effects of two weight-loss management strategies, one with anti-obesity medication and one without, against a traditional glucose-centric care approach in patients with obesity and type 2 diabetes.
- - Conducted at the Cleveland Clinic, the trial faced challenges during enrollment and retention due to COVID-19, resulting in the participation of only 74 out of 300 planned individuals.
- - Results showed that after 12 months, participants using the weight management program with anti-obesity medication lost more weight than those receiving usual care, while both obesity-centric approaches proved effective in reducing HbA levels.